CD15 antibody

Catalog No. ABIN967638

Product Details anti-CD15 Antibody

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD15 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Functional Studies (Func)

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: U-937 histiocytic cell line

Clone: MMA

Isotype: IgM kappa

Application Details

Application Notes: Functional Studies:
Anti-Lewis[x] monoclonal antibody (clone MMA) has been used to determine Le[x] expression on normal and neoplastic cells in studies of tumor metastasis, together with sialyl-Le[x] (CD15s), E-selectin (CD62E) and Pselectin (CD62P) monoclonal antibodies.

Immunohistology:
Anti-Lewis[x] monoclonal antibody (clone MMA) can be used in staining formalin-fixed paraffin-embedded tissue sections of normal prostate, colon, stomach, brain and lymphoid tissues, adenocarcinomas of lung and urinary bladder. The MMA monoclonal antibody also stains neuraminidase-treated, formalin-fixed, paraffin-embedded sections of normal, metaplastic and neoplastic cervical epithelium.

Immunoblotting:
Anti-Lewis[x] monoclonal antibody (clone MMA) can be used to detect Le[x] in membranous glycoprotein cell or tissue extracts by immunoblotting.

Cell ELISA:
Anti-Lewis[x] monoclonal antibody (clone MMA) can be used to detect Le[x] on HL-60 promyelocytic, HT-29 colon cancer or F9 teratocarcinoma cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD15 antibody (ABIN967638)

Puller, Ondreka, Haverkamp: "Bipolar cells of the ground squirrel retina." in: The Journal of comparative neurology, Vol. 519, Issue 4, pp. 759-74, (2011) (PubMed).

Koizumi, Jakobs, Masland: "Regular mosaic of synaptic contacts among three retinal neurons." in: The Journal of comparative neurology, Vol. 519, Issue 2, pp. 341-57, (2011) (PubMed).

Puller, Haverkamp, Grünert: "OFF midget bipolar cells in the retina of the marmoset, Callithrix jacchus, express AMPA receptors." in: The Journal of comparative neurology, Vol. 502, Issue 3, pp. 442-54, (2007) (PubMed).

Bevilacqua, Nelson: "Endothelial-leukocyte adhesion molecules in inflammation and metastasis." in: Thrombosis and haemostasis, Vol. 70, Issue 1, pp. 152-4, (1993) (PubMed).

García Prats, López Carreira, Martínez-González, Ballestín, Gil, De Prada: "Granular cell basal cell carcinoma. Light microscopy, immunohistochemical and ultrastructural study." in: Virchows Archiv. A, Pathological anatomy and histopathology, Vol. 422, Issue 2, pp. 173-7, (1993) (PubMed).

Groves, Allen, Ross, Ahsan, Barker, MacDonald: "Expression of selectin ligands by cutaneous squamous cell carcinoma." in: The American journal of pathology, Vol. 143, Issue 4, pp. 1220-5, (1993) (PubMed).

Ma, Zarbo, Frierson, Lee: "Comparative immunohistochemical study of primary and metastatic carcinomas of the liver." in: American journal of clinical pathology, Vol. 99, Issue 5, pp. 551-7, (1993) (PubMed).

Narita, Funahashi, Satoh, Watanabe, Sakamoto, Takagi: "Association of expression of blood group-related carbohydrate antigens with prognosis in breast cancer." in: Cancer, Vol. 71, Issue 10, pp. 3044-53, (1993) (PubMed).

Paties, Taccagni, Papotti, Valente, Zangrandi, Aloi: "Apocrine carcinoma of the skin. A clinicopathologic, immunocytochemical, and ultrastructural study." in: Cancer, Vol. 71, Issue 2, pp. 375-81, (1993) (PubMed).

Torii, Nakayama, Harada, Nakao, Nonami, Sakamoto, Watanabe, Ito, Takagi: "Expression of the CD15 antigen in hepatocellular carcinoma." in: Cancer, Vol. 71, Issue 12, pp. 3864-7, (1993) (PubMed).

McCormick, Zetter: "Adhesive interactions in angiogenesis and metastasis." in: Pharmacology & therapeutics, Vol. 53, Issue 2, pp. 239-60, (1992) (PubMed).

Grignon, Ro, Ayala, Johnson, Ordóñez: "Primary adenocarcinoma of the urinary bladder. A clinicopathologic analysis of 72 cases." in: Cancer, Vol. 67, Issue 8, pp. 2165-72, (1991) (PubMed).

Zhou, Moore, Smith, Varki, McEver, Cummings: "The selectin GMP-140 binds to sialylated, fucosylated lactosaminoglycans on both myeloid and nonmyeloid cells." in: The Journal of cell biology, Vol. 115, Issue 2, pp. 557-64, (1991) (PubMed).

OHara, Corson, Pinkus, Stahel: "ME1. A monoclonal antibody that distinguishes epithelial-type malignant mesothelioma from pulmonary adenocarcinoma and extrapulmonary malignancies." in: The American journal of pathology, Vol. 136, Issue 2, pp. 421-8, (1990) (PubMed).

Hsu, Huang, Hsu, Ge, Ho, Cuttita, Mulshine: "Biochemical and ultrastructural study of Leu M1 antigen in Reed-Sternberg cells: comparison with granulocytes and interdigitating reticulum cells." in: Journal of the National Cancer Institute, Vol. 77, Issue 2, pp. 363-70, (1986) (PubMed).

Hanjan, Kearney, Cooper: "A monoclonal antibody (MMA) that identifies a differentiation antigen on human myelomonocytic cells." in: Clinical immunology and immunopathology, Vol. 23, Issue 2, pp. 172-88, (1982) (PubMed).

Target Details for CD15

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Alternative Name: CD15 (FUT4 Products)

Synonyms: AI451562 antibody, CD15 antibody, FAL antibody, FucT-IV antibody, LeX antibody, SSEA-1 antibody, Ssea1 antibody, ELFT antibody, FCT3A antibody, FUC-TIV antibody, FUTIV antibody, Fuct antibody, fucosyltransferase 4 antibody, Fut4 antibody, FUT4 antibody

Background: Reacts with the Le[x] blood group antigen, lacto-N-fucopentaose III or the CD15 antigen. The Le[x] determinant is expressed on normal cells and tissues of diverse origin, including myelomonocytic cells, myoepithelial cells in prostate, glandular epithelial cells including breast, renal cell and hepatocellular carcinomas, lung and urinary bladder adenocarcinomas and Reed-Sternberg cells in Hodgkin's disease.