FUT4 antibody (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific)) (FITC)

Details for Product anti-FUT4 Antibody No. ABIN93987
Antigen
Reactivity
Human
456
81
71
17
3
1
Host
Mouse
363
97
Clonality (Clone)
Monoclonal ()
Conjugate
This FUT4 antibody is conjugated to FITC
53
32
30
15
8
6
6
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
3
2
2
2
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Microcytotoxicity Assay (MCA)
338
163
150
68
52
47
32
22
18
14
13
13
8
6
6
6
4
3
1
1
Options
Immunogen Human granulocytes
Clone MEM-158
Isotype IgM
Specificity The antibody MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells, it is also present on Langerhans cells and some myeloid precursors cells.
HLDA VI, WS Code AS A053
No Cross-Reactivity Pig (Porcine), Cow (Bovine), Sheep (Ovine)
Cross-Reactivity (Details) Human
Antigen
Alternative Name CD15 (FUT4 Antibody Abstract)
Background CD15 (Lewis X, Le(x), stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Comment

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in stabilizing Tris buffered saline (TBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibody (FITC) (ABIN93987) Flow Cytometry analysis Surface staining (flow cytometry) of human peripheral blood c...
Image no. 2 for anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibody (FITC) (ABIN93987) Surface staining (mass cytometry) of human peripheral blood cells (after ammonium chl...