FUT4 antibody (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific)) (Biotin)

Details for Product anti-FUT4 Antibody No. ABIN93986
Antigen
Reactivity
Human
455
81
71
17
3
1
Host
Mouse
362
97
Clonality (Clone)
Monoclonal ()
Conjugate
This FUT4 antibody is conjugated to Biotin
54
31
30
14
8
6
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5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
3
2
2
2
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Microcytotoxicity Assay (MCA)
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163
150
68
52
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32
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Options
Immunogen Human granulocytes
Clone MEM-158
Isotype IgM
Specificity The antibody MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells, it is also present on Langerhans cells and some myeloid precursors cells.
HLDA VI, WS Code AS A053
No Cross-Reactivity Pig (Porcine), Cow (Bovine), Sheep (Ovine)
Cross-Reactivity (Details) Human
Antigen
Alternative Name CD15 (FUT4 Antibody Abstract)
Background CD15 (Lewis X, Le(x), stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.
Application Notes Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry.Suggested working dilution is 1:1000. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Comment

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibody (Biotin) (ABIN93986) Flow Cytometry analysis Surface staining (flow cytometry) of human peripheral blood c...
Image no. 2 for anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibody (Biotin) (ABIN93986) Surface staining (mass cytometry) of human peripheral blood cells (after ammonium chl...