Cyclin-Dependent Kinase 4 (CDK4) antibody

Details for Product No. ABIN967647
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Antigen
Synonyms zgc:153726, CMM3, PSK-J3, Crk3, 8-6, CDK4, CDK4/6, CG5072, Cdk4/6, DmCdk4, Dmel\\CG5072, Pk53C, Pk?7, cdk, cdk4, cdk4/6, dCdk4, l(2)05428, l(2)0671, l(2)k06503, l(2)s4639, l(2)sh0671, xcdk4
Reactivity
Human
(178), (64), (49), (19), (14), (4), (1)
Host
Mouse
(104), (83), (5), (2), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(7), (5), (5), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
(178), (62), (55), (41), (29), (18), (17), (11), (10), (10), (8), (5), (2), (2), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN967647
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Immunogen Recombinant Human Cdk4
Clone DCS-156
Isotype IgG1
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Cdk4
Background Cyclins, cyclin-dependent kinases (Cdks), and cyclin-dependent kinase inhibitors (CdkIs) are essential for cell-cycle control in eukarytotes. Cyclins, regulatory subunits, bind to cyclin-dependent kinases (Cdks), catalytic subunits, to form active cyclin-Cdk complexes. Cdk subunits by themselves are inactive and binding to a cyclin is required for their activity. Cyclins A, B1, D and E undergo periodic synthesis and degradation, thereby providing a mechanism to regulate Cdk activity throughout the cell cycle. Additionally, Cdk activity is further regulated by activating and inhibitory phosphorylations, and small proteins (p15, p16, p18, p19, p21 and p27), called inhibitors of Cdk activity, that bind to cyclins, Cdks, or cyclin- Cdk complexes. Cdk4 was originally called PSK-J3, and following demonstration of its association with D-type cyclins, became known as Cdk4. D-type cyclins also associate with Cdks 2 and 5, although Cdk4 appears to be the most abundant partner. The D-type cyclins (D1, D2, and D3) are expressed in response to growth factors or mitogens, and rapidly degrade when mitogens are withdrawn. D cyclins appear to promote G0 to G1 transitions and the rate of G1 progression. For example, cyclin D-Cdk4 and cyclin D-Cdk6 complexes phosphorylate the retinoblastoma protein (Rb) which removes the G1 phase block caused by underphosphorylated Rb. Cdk4 has a molecular weight of ~32 kD. Clone DCS-156 recognizes human and mouse Cdk4. A recombinant protein fragment from the C-terminal end of human Cdk4 was used as immunogen.
Molecular Weight 32 kDa
Research Area Cancer, Cell Cycle, Kinases/Phosphatases
Application Notes Applications include western blot analysis (0.5-2.0 µg/ml) and immunoprecipitation (1.0 µg/one million cells). The antibody has also been used for immunohistochemistry of formalin-fixed, paraffin-embedded tissue sections, but this application is not routinely tested. HeLa (ATCC CCL 2), 293 (ATCC CRL 1573), or NIH/3T3 (ATCC CRL 1658) cells are suggested as positive controls.
Comment

Related Products: ABIN967389, ABIN968535

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Cyclin-Dependent Kinase 4 (CDK4) antibody Immunoprecipitiation/western blot analysis of Cdk4. HeLa or 293 cell lysates were immunoprecipitated with an antibody to Cdk4 (clone DCS-156). Proteins were separated by SDS/PAGE and then western blotted with clone DCS-156. The bands above and below the ~32 kD Cdk4 bands, represent the heavy and light chains of IgG used for immunoprecipitation.
anti-Cyclin-Dependent Kinase 4 (CDK4) antibody (2) Western blot titration of anti-Cdk4 antibody. HeLa or 293 cell lysates were probed with 6 ug/ml (lanes 1 and 4), 2.0 µg/ml (lanes 2 and 4) or 0.5 µg/ml (lanes 3 and 5) of antibody (clone DCS-156). The antibody identifies Cdk4 at ~32 kD.
Product cited in: Matsushime, Ewen, Strom et al.: "Identification and properties of an atypical catalytic subunit (p34PSK-J3/cdk4) for mammalian D type G1 cyclins." in: Cell, Vol. 71, Issue 2, pp. 323-34, 1992 (PubMed).

Johnson, Walker: "Cyclins and cell cycle checkpoints." in: Annual review of pharmacology and toxicology, Vol. 39, pp. 295-312, 1999 (PubMed).

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