FOXA2 antibody

Catalog No. ABIN967680

This anti-FOXA2 antibody is a Mouse Monoclonal antibody detecting FOXA2 in WB, BI and ICS. Suitable for Human and Mouse. This Primary Antibody has been cited in 7+ publications.

Quick Overview for FOXA2 antibody (ABIN967680)

Target: FOXA2 (Forkhead Box A2 (FOXA2))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This FOXA2 antibody is un-conjugated

Application: Western Blotting (WB), BioImaging (BI), Intracellular Staining (ICS)

Clone: N17-280

Product Details anti-FOXA2 Antibody

Brand: BD Pharmingen™

Cross-Reactivity (Details): Predicted: Mouse

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Triton is a trademark of the Dow Chemical Company.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human FoxA2 Peptide

Isotype: IgG1 kappa

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-FOXA2 antibody (ABIN967680)

Burtscher, Lickert: "Foxa2 regulates polarity and epithelialization in the endoderm germ layer of the mouse embryo." in: Development (Cambridge, England), Vol. 136, Issue 6, pp. 1029-38, (2009) (PubMed).

Lin, Metzakopian, Mavromatakis, Gao, Balaskas, Sasaki, Briscoe, Whitsett, Goulding, Kaestner, Ang: "Foxa1 and Foxa2 function both upstream of and cooperatively with Lmx1a and Lmx1b in a feedforward loop promoting mesodiencephalic dopaminergic neuron development." in: Developmental biology, Vol. 333, Issue 2, pp. 386-96, (2009) (PubMed).

DAmour, Agulnick, Eliazer, Kelly, Kroon, Baetge: "Efficient differentiation of human embryonic stem cells to definitive endoderm." in: Nature biotechnology, Vol. 23, Issue 12, pp. 1534-41, (2005) (PubMed).

Thomson, Itskovitz-Eldor, Shapiro, Waknitz, Swiergiel, Marshall, Jones: "Embryonic stem cell lines derived from human blastocysts." in: Science (New York, N.Y.), Vol. 282, Issue 5391, pp. 1145-7, (1998) (PubMed).

Monaghan, Kaestner, Grau, Schütz: "Postimplantation expression patterns indicate a role for the mouse forkhead/HNF-3 alpha, beta and gamma genes in determination of the definitive endoderm, chordamesoderm and neuroectoderm." in: Development (Cambridge, England), Vol. 119, Issue 3, pp. 567-78, (1994) (PubMed).

Clevidence, Overdier, Tao, Qian, Pani, Lai, Costa: "Identification of nine tissue-specific transcription factors of the hepatocyte nuclear factor 3/forkhead DNA-binding-domain family." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 9, pp. 3948-52, (1993) (PubMed).

Lai, Prezioso, Tao, Chen, Darnell: "Hepatocyte nuclear factor 3 alpha belongs to a gene family in mammals that is homologous to the Drosophila homeotic gene fork head." in: Genes & development, Vol. 5, Issue 3, pp. 416-27, (1991) (PubMed).

Target Details for FOXA2

Target: FOXA2 (Forkhead Box A2 (FOXA2))

Alternative Name: FOXA2

Background: FoxA2, forkhead box A2, is a member of the forkhead class of DNA-binding proteins that regulates gene expression in the liver, pancreatic islets, adipocytes and some neural cells. This hepatocyte nuclear factor is a transcriptional activator for liver-specific genes such as alpha fetoprotein, albumin, tyrosine aminotransferase and transthyretin. FoxA2 is expressed in embryonic endoderm, the germ layer that gives rise to the digestive system, and contributes to the specification of the pancreas and the regulation of glucose homoeostasis. FoxA2 also has roles in neural development. Specifically, FoxA2 cooperates with related FoxA1 in the specification and differentiation of midbrain dopaminergic neurons in a dosage-dependent manner.
Synonyms: Forkhead box A2, HNF3B, HNF-3B, HNF-3beta, hepatocyte nuclear factor 3beta

Molecular Weight: 48 kDa

Pathways: Dopaminergic Neurogenesis, Regulation of Carbohydrate Metabolic Process