C-Raf-1 (AA 162-378) antibody

Catalog No. ABIN967782

This anti- antibody is a Mouse Monoclonal antibody detecting in IF, IP, IHC and WB. Suitable for Chicken, Dog, Human, Mouse and Rat. This Primary Antibody has been cited in 5+ publications.

Quick Overview for C-Raf-1 (AA 162-378) antibody (ABIN967782)

Target: C-Raf-1

Reactivity: Chicken, Dog, Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)

Clone: 53-c-Raf

Product Details

Binding Specificity: AA 162-378

Cross-Reactivity: Dog (Canine), Rat (Rattus), Mouse (Murine), Chicken

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human c-Raf-1 aa. 162-378

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968533

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References

Coles, Shaw: "PAK1 primes MEK1 for phosphorylation by Raf-1 kinase during cross-cascade activation of the ERK pathway." in: Oncogene, Vol. 21, Issue 14, pp. 2236-44, (2002) (PubMed).

Rizzo, Kraft, Watkins, Levitan, Romero: "Agonist-dependent traffic of raft-associated Ras and Raf-1 is required for activation of the mitogen-activated protein kinase cascade." in: The Journal of biological chemistry, Vol. 276, Issue 37, pp. 34928-33, (2001) (PubMed).

Cheng, Wung, Chao, Wang: "Sequential activation of protein kinase C (PKC)-alpha and PKC-epsilon contributes to sustained Raf/ERK1/2 activation in endothelial cells under mechanical strain." in: The Journal of biological chemistry, Vol. 276, Issue 33, pp. 31368-75, (2001) (PubMed).

Bondzi, Grant, Krystal: "A novel assay for the measurement of Raf-1 kinase activity." in: Oncogene, Vol. 19, Issue 43, pp. 5030-3, (2000) (PubMed).

Rapp: "Role of Raf-1 serine/threonine protein kinase in growth factor signal transduction." in: Oncogene, Vol. 6, Issue 4, pp. 495-500, (1991) (PubMed).

Target Details

Target: C-Raf-1

Background: Raf (c-Raf-1) is a cytoplasmic serine/threonine protein kinase and is a member of a family of proteins which are highly conserved from Drosophila to mammals. Raf has a critical role in the response to many growth factors including: EGF, PDGF, insulin, IL-2, IL-3, CSF-1, and GM-CSF. Raf can directly interact with Ras-GTP and subsequently become activated. Thus Raf plays a prominent role in the Ras signaling pathway by transferring a signal from Ras which has been activated by growth factor receptor-stimulated tyrosine kinase activity. This leads to the stimulation and activation of a number of other cytoplasmic serine/threonine kinases. Raf regulates the MAP kinase pathway by phosphorylating and activating MEK, which then phosphorylates and activates MAP kinase (ERK). ERK then phosphorylates and activates Rsk. Raf activity can also be regulated independently of Ras. Cyclic AMP (cAMP) activation of protein kinase A (PKA) can inhibit growth factor stimulation of Raf.

Molecular Weight: 74 kDa