PKA R1 alpha (PKR1) (AA 1-381) antibody

Catalog No. ABIN968062

This Mouse Monoclonal antibody specifically detects in IF, IP, IHC and WB. It exhibits reactivity toward Chicken, Dog, Human, Mouse and Rat and has been mentioned in 5+ publications.

Quick Overview for PKA R1 alpha (PKR1) (AA 1-381) antibody (ABIN968062)

Target: PKA R1 alpha (PKR1)

Reactivity: Chicken, Dog, Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)

Clone: 20-PKA RIalpha

Product Details

Binding Specificity: AA 1-381

Cross-Reactivity: Rat (Rattus), Dog (Canine), Mouse (Murine), Chicken

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human PKA [RIalpha] aa. 1-381

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References

Taskén, Collas, Kemmner, Witczak, Conti, Taskén: "Phosphodiesterase 4D and protein kinase a type II constitute a signaling unit in the centrosomal area." in: The Journal of biological chemistry, Vol. 276, Issue 25, pp. 21999-2002, (2001) (PubMed).

Vang, Torgersen, Sundvold, Saxena, Levy, Skålhegg, Hansson, Mustelin, Taskén: "Activation of the COOH-terminal Src kinase (Csk) by cAMP-dependent protein kinase inhibits signaling through the T cell receptor." in: The Journal of experimental medicine, Vol. 193, Issue 4, pp. 497-507, (2001) (PubMed).

Casey, Vaughan, He, Hatcher, Winter, Weremowicz, Montgomery, Kucherlapati, Morton, Basson: "Mutations in the protein kinase A R1alpha regulatory subunit cause familial cardiac myxomas and Carney complex." in: The Journal of clinical investigation, Vol. 106, Issue 5, pp. R31-8, (2000) (PubMed).

Skålhegg, Landmark, Foss, Lohmann, Hansson, Lea, Jahnsen: "Identification, purification, and characterization of subunits of cAMP-dependent protein kinase in human testis. Reverse mobilities of human RII alpha and RII beta on sodium dodecyl sulfate-polyacrylamide gel electrophoresis compared with rat and bovine R" in: The Journal of biological chemistry, Vol. 267, Issue 8, pp. 5374-9, (1992) (PubMed).

Sandberg, Skålhegg, Jahnsen: "The two mRNA forms for the type I alpha regulatory subunit of cAMP-dependent protein kinase from human testis are due to the use of different polyadenylation site signals." in: Biochemical and biophysical research communications, Vol. 167, Issue 1, pp. 323-30, (1990) (PubMed).

Target Details

Target: PKA R1 alpha (PKR1)

Alternative Name: PKA RI alpha

Background: CAMP-dependent Protein Kinase (PKA) is composed of two distinct subunits: catalytic (C) and regulatory (R). Four regulatory subunits have been identified: RIalpha, RIbeta, RIIalpha, and RIIbeta. These subunits define type I and II cAMP-dependent protein kinases. Following binding of cAMP, the regulatory subunits dissociate from the catalytic subunits, rendering the enzyme active. Type I and type II holoenzymes have three potential C subunits (Calpha, Cbeta, or Cgamma). Type II PKA can be distinguished by autophosphorylation of the R-subunits, while type I PKA binds Mg/ATP with high affinity. Most cells express both type I and type II PKAs. Although the Ralpha isoforms are ubiquitously expressed, the Rbeta isoforms are predominant in nervous and adipose tissues. Expression of the RIbeta subunit is modulated during muscle and adipocyte differentiation in vitro.

Molecular Weight: 49 kDa