Myeloid/lymphoid Or Mixed-Lineage Leukemia (Trithorax Homolog, Drosophila), Translocated To, 4 (MLLT4) (AA 1091-1233) antibody

Details for Product No. ABIN968143
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Antigen
Synonyms AF6, RP3-431P23.3, 5033403D15Rik, Af-6, Afadin, Gm314, I-afadin, S-afadin, Af6, MLLT4, af-6, af6, si:ch211-278j12.1
Epitope
AA 1091-1233
(12), (3), (3), (1), (1), (1)
Reactivity
Human
(43), (29), (29), (13), (12), (12), (1), (1)
Host
Mouse
(42), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunofluorescence (IF)
(20), (18), (14), (11), (2), (2), (1), (1)
Pubmed 5 references available
Catalog no. ABIN968143
Quantity 50 µg
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Immunogen Human AF6
Clone G52C7-1
Isotype IgG2a
Cross-Reactivity Rat (Rattus), Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name AF6
Background AF6 (ALL-1 Fusion partner in chromosome 6) was discovered as the fusion partner of ALL-1. These genes undergo translocation and fusion in acute myeloid leukemias. AF6, or p180, was also identified as a Ras-interacting protein isolated from bovine brain. AF6 binds to the GTP-Ras but not to the inactive GDP, or ineffective forms of Ras. The interaction between AF6 and Ras may regulate the Ras signaling pathway since this association was prevented by the binding of c-Raf-1 to Ras. The Ras-binding site is located within amino acids 36-206 of AF6. AF6 is 1612 amino acids in length with homology to the DLG family of proteins located in cell-cell junctions, suggesting AF6 might be important in cell-to-cell communicative events. Furthermore, AF6 has the GLGF motif characteristic of DLG proteins that include Drosophilia's Canoe, implicated in the developmental pathway triggered by Notch activation. Like AF6, Canoe was also identified as a Ras-binding protein. The amino acid sequence and biochemical property similarities between Canoe and AF6 indicate that the latter may be important in developmental processes regulated by Ras.
Synonyms: ALL-1 Fusion partner in chromosome 6
Molecular Weight 182 kDa
Comment

Related Products: ABIN968545, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Storage -20 °C
Supplier Images
anti-Myeloid/lymphoid Or Mixed-Lineage Leukemia (Trithorax Homolog, Drosophila), Translocated To, 4 (MLLT4) (AA 1091-1233) antibody Western blot analysis of AF6 on a PC-12 cell lysate (Rat neuroblastoma, ATCC CRL-1721). Lane 1: 1:125, lane 2: 1:250, lane 3: 1:500 dilution of the mouse anti-AF6 antibody.
anti-Myeloid/lymphoid Or Mixed-Lineage Leukemia (Trithorax Homolog, Drosophila), Translocated To, 4 (MLLT4) (AA 1091-1233) antibody (2) Immunofluorescence staining of mouse macrophages.
Product cited in: Prasad, Gu, Alder et al.: "Cloning of the ALL-1 fusion partner, the AF-6 gene, involved in acute myeloid leukemias with the t(6;11) chromosome translocation." in: Cancer research, Vol. 53, Issue 23, pp. 5624-8, 1994 (PubMed).

Kuriyama, Harada, Kuroda et al.: "Identification of AF-6 and canoe as putative targets for Ras." in: The Journal of biological chemistry, Vol. 271, Issue 2, pp. 607-10, 1996 (PubMed).

Buchert, Schneider, Meskenaite et al.: "The junction-associated protein AF-6 interacts and clusters with specific Eph receptor tyrosine kinases at specialized sites of cell-cell contact in the brain." in: The Journal of cell biology, Vol. 144, Issue 2, pp. 361-71, 1999 (PubMed).

Cordenonsi, DAtri, Hammar et al.: "Cingulin contains globular and coiled-coil domains and interacts with ZO-1, ZO-2, ZO-3, and myosin." in: The Journal of cell biology, Vol. 147, Issue 7, pp. 1569-82, 2000 (PubMed).

Ebnet, Schulz, Meyer Zu Brickwedde et al.: "Junctional adhesion molecule interacts with the PDZ domain-containing proteins AF-6 and ZO-1." in: The Journal of biological chemistry, Vol. 275, Issue 36, pp. 27979-88, 2000 (PubMed).

Hosts (42), (1)
Reactivities (43), (29), (29), (13), (12), (12), (1), (1)
Applications (20), (18), (14), (11), (2), (2), (1), (1)
Conjugates (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (12), (3), (3), (1), (1), (1)
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