Afadin antibody (N-Term)

Catalog No. ABIN2778340

The Rabbit Polyclonal anti-Afadin antibody (ABIN2778340) specifically detects Afadin in WB. The antibody is reactive with Human, Rat, Mouse, Dog, Cow, Guinea Pig, Horse, Rabbit, Pig, Saccharomyces cerevisiae and Zebrafish (Danio rerio) samples.

Quick Overview for Afadin antibody (N-Term) (ABIN2778340)

Target: Afadin (MLLT4)

Reactivity: Human, Rat, Mouse, Dog, Cow, Guinea Pig, Horse, Rabbit, Pig, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Afadin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Afadin Antibody

Binding Specificity: N-Term

Purpose: AFDN Antibody - N-terminal region

Sequence: GVIQNFKRTL SKKEKKEKKK REKEALRQAS DKDDRPFQGE DVENSRLAAE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 92%, Horse: 93%, Human: 100%, Mouse: 93%, Pig: 93%, Rabbit: 85%, Rat: 100%, Yeast: 92%, Zebrafish: 91%

Characteristics: This is a rabbit polyclonal antibody against MLLT4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MLLT4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8094301

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for Afadin

Target: Afadin (MLLT4)

Alternative Name: AFDN

Background:

Background Information: This gene encodes a multi-domain protein involved in signaling and organization of cell junctions during embryogenesis. It has also been identified as the fusion partner of acute lymphoblastic leukemia (ALL-1) gene, involved in acute myeloid leukemias with t(6,11)(q27,q23) translocation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, however, not all have been fully characterized.

Gene Name: afadin, adherens junction formation factor

Alternative Symbols: AF6, MLLT4, MLL-AF6, l-afadin

Protein Name: afadin

Molecular Weight: 74kDa

Gene ID: 4301

UniProt: P55196

Pathways: Cell-Cell Junction Organization