NCF2 antibody (AA 317-469)

Catalog No. ABIN968246

The Mouse Monoclonal anti-NCF2 antibody has been validated for WB and IF. It is suitable to detect NCF2 in samples from Human and Mouse. There are 5+ publications available.

Quick Overview for NCF2 antibody (AA 317-469) (ABIN968246)

Target: NCF2 (Neutrophil Cytosolic Factor 2 (NCF2))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This NCF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 29-p67phox

Product Details anti-NCF2 Antibody

Binding Specificity: AA 317-469

Cross-Reactivity: Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human p67 [phox] aa. 317-469

Isotype: IgG2b

Application Details

Comment:

Related Products: ABIN968584, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-NCF2 antibody (ABIN968246)

Ago, Nunoi, Ito, Sumimoto: "Mechanism for phosphorylation-induced activation of the phagocyte NADPH oxidase protein p47(phox). Triple replacement of serines 303, 304, and 328 with aspartates disrupts the SH3 domain-mediated intramolecular interaction in p47(phox), thereby activating" in: The Journal of biological chemistry, Vol. 274, Issue 47, pp. 33644-53, (1999) (PubMed).

Benna, Dang, Gaudry, Fay, Morel, Hakim, Gougerot-Pocidalo: "Phosphorylation of the respiratory burst oxidase subunit p67(phox) during human neutrophil activation. Regulation by protein kinase C-dependent and independent pathways." in: The Journal of biological chemistry, Vol. 272, Issue 27, pp. 17204-8, (1997) (PubMed).

Freeman, Lambeth: "NADPH oxidase activity is independent of p47phox in vitro." in: The Journal of biological chemistry, Vol. 271, Issue 37, pp. 22578-82, (1996) (PubMed).

Leto, Adams, de Mendez: "Assembly of the phagocyte NADPH oxidase: binding of Src homology 3 domains to proline-rich targets." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 22, pp. 10650-4, (1994) (PubMed).

Leto, Lomax, Volpp, Nunoi, Sechler, Nauseef, Clark, Gallin, Malech: "Cloning of a 67-kD neutrophil oxidase factor with similarity to a noncatalytic region of p60c-src." in: Science (New York, N.Y.), Vol. 248, Issue 4956, pp. 727-30, (1990) (PubMed).

Target Details for NCF2

Target: NCF2 (Neutrophil Cytosolic Factor 2 (NCF2))

Alternative Name: p67 phox

Background: The neutrophil respiratory burst oxidase (NADPH-oxidase) generates superoxide and secondary oxygen-derived toxic products in response to bacteria or a variety of soluble stimuli. The active site of this enzyme is located in an integral membrane cytochrome, b558, that consists of the two subunits gp91 [phox] and p21 [phox]. Superoxide production depends on the formation of a complex that includes p67 [phox], p47 [phox], and the GTP-binding protein Rac. Upon activation, these proteins translocate from the cytosol to the membrane where they assemble with b558 and induce oxidase activity. p67 [phox] contains two SH3 domains and binds, via its C-terminal SH3 domain, to the proline rich region of p47 [phox]. This binding allows p67 [phox] to indirectly associate with the oxidase. It is thought that the phosphorylated forms of p67 [phox] and p47 [phox] interact and that the phosphorylation of p67 [phox] is regulated by both PKC-dependent and independent pathways. Although the role of p67 [phox] in electron flow control is poorly understood, it is thought that it regulates the transfer of electrons from NADPH to reduce flavin.

Molecular Weight: 67 kDa