P4HB antibody (AA 109-214)

Catalog No. ABIN968268

This anti-P4HB antibody is a Mouse Monoclonal antibody detecting P4HB in WB and IF. Suitable for Human, Mouse, Rat and Dog. This Primary Antibody has been cited in 5+ publications.

Quick Overview for P4HB antibody (AA 109-214) (ABIN968268)

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This P4HB antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 34-PDI

Product Details anti-P4HB Antibody

Binding Specificity: AA 109-214

Cross-Reactivity: Human, Dog (Canine), Rat (Rattus), Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Bovine PDI aa. 109-214

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968551, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-P4HB antibody (ABIN968268)

Jenne, Frey, Brugger, Wieland: "Oligomeric state and stoichiometry of p24 proteins in the early secretory pathway." in: The Journal of biological chemistry, Vol. 277, Issue 48, pp. 46504-11, (2002) (PubMed).

Schlegel, Arvan, Lisanti: "Caveolin-1 binding to endoplasmic reticulum membranes and entry into the regulated secretory pathway are regulated by serine phosphorylation. Protein sorting at the level of the endoplasmic reticulum." in: The Journal of biological chemistry, Vol. 276, Issue 6, pp. 4398-408, (2001) (PubMed).

Weissman, Kim: "Efficient catalysis of disulphide bond rearrangements by protein disulphide isomerase." in: Nature, Vol. 365, Issue 6442, pp. 185-8, (1993) (PubMed).

Wetterau, Combs, Spinner, Joiner: "Protein disulfide isomerase is a component of the microsomal triglyceride transfer protein complex." in: The Journal of biological chemistry, Vol. 265, Issue 17, pp. 9800-7, (1990) (PubMed).

Yamauchi, Yamamoto, Hayashi, Koya, Takikawa, Toyoshima, Horiuchi: "Sequence of membrane-associated thyroid hormone binding protein from bovine liver: its identity with protein disulphide isomerase." in: Biochemical and biophysical research communications, Vol. 146, Issue 3, pp. 1485-92, (1987) (PubMed).

Target Details for P4HB

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Alternative Name: PDI

Background: The ER is the site of translation of membrane and secretory proteins. Following synthesis, it shuttles these proteins through a contiguous membrane system to their appropriate destinations. Protein disulfide isomerase (PDI) is an abundant, multifunctional, eukaryotic protein. Although it exhibits ubiquitous expression, it is primarily located in the ER lumen. Here, it functions to catalyze the isomerization of intramolecular disulfide bridges, thereby allowing them to generate their most thermodynamically stable configurations. This role in rearrangement has lead to the classification of PDI as a chaperone. Although protein folding occurs in its absence, PDI may be essential for it to proceed at a physiological relevant rate. In addition, PDI is the beta-subunit of prolyl 4- hydroxylase and is a component of the triglyceride transfer complex. PDI is retained in the ER lumen via its C-terminal -KDEL sequence. Via this sequence, it is continuously recycled back to the ER from other membranous compartments. Thus, PDI is a diverse protein whose primary function may be to correct disulfide bonding and, thus, ensure the most stable conformation of newly synthesized proteins.

Molecular Weight: 55 kDa

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism