Caveolin antibody (pTyr14)

Catalog No. ABIN968480

The Mouse Monoclonal anti-Caveolin antibody has been validated for WB, IF and IHC (zinc). It is suitable to detect Caveolin in samples from Human, Mouse and Rat. There are 6+ publications available.

Quick Overview for Caveolin antibody (pTyr14) (ABIN968480)

Target: Caveolin (CAV)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caveolin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Clone: 56-Caveolin

Product Details anti-Caveolin Antibody

Binding Specificity: pTyr14

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human Caveolin-1 (Y14) Peptide

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389, ABIN968534

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-Caveolin antibody (ABIN968480)

Hill, Scherbakov, Schiefermeier, Baran, Hancock, Huber, Parton, Parat: "Reassessing the role of phosphocaveolin-1 in cell adhesion and migration." in: Traffic (Copenhagen, Denmark), Vol. 8, Issue 12, pp. 1695-705, (2007) (PubMed).

Labrecque, Royal, Surprenant, Patterson, Gingras, Béliveau: "Regulation of vascular endothelial growth factor receptor-2 activity by caveolin-1 and plasma membrane cholesterol." in: Molecular biology of the cell, Vol. 14, Issue 1, pp. 334-47, (2003) (PubMed).

Lee, Volonte, Galbiati, Iyengar, Lublin, Bregman, Wilson, Campos-Gonzalez, Bouzahzah, Pestell, Scherer, Lisanti: "Constitutive and growth factor-regulated phosphorylation of caveolin-1 occurs at the same site (Tyr-14) in vivo: identification of a c-Src/Cav-1/Grb7 signaling cassette." in: Molecular endocrinology (Baltimore, Md.), Vol. 14, Issue 11, pp. 1750-75, (2001) (PubMed).

Ushio-Fukai, Hilenski, Santanam, Becker, Ma, Griendling, Alexander: "Cholesterol depletion inhibits epidermal growth factor receptor transactivation by angiotensin II in vascular smooth muscle cells: role of cholesterol-rich microdomains and focal adhesions in angiotensin II signaling." in: The Journal of biological chemistry, Vol. 276, Issue 51, pp. 48269-75, (2001) (PubMed).

Volonté, Galbiati, Pestell, Lisanti: "Cellular stress induces the tyrosine phosphorylation of caveolin-1 (Tyr(14)) via activation of p38 mitogen-activated protein kinase and c-Src kinase. Evidence for caveolae, the actin cytoskeleton, and focal adhesions as mechanical sensors of osmotic stres" in: The Journal of biological chemistry, Vol. 276, Issue 11, pp. 8094-103, (2001) (PubMed).

Glenney, Soppet: "Sequence and expression of caveolin, a protein component of caveolae plasma membrane domains phosphorylated on tyrosine in Rous sarcoma virus-transformed fibroblasts." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 21, pp. 10517-21, (1992) (PubMed).

Target Details for Caveolin

Target: Caveolin (CAV)

Alternative Name: Caveolin

Background: Caveolin (VIP21) localizes to non-clathrin membrane invaginations (caveolae) on the inner surface of the plasma membrane. In addition, it is present in the trans-Golgi network (TGN) and in apically and basolaterally destined transport vesicles. Caveolin is a transmembrane adaptor molecule that recognizes GPI-linked proteins and interacts with downstream cytoplasmic signaling molecules, such as src-family tyrosine kinases and hetero-trimeric G proteins. Caveolin forms large lipid-binding oligomers, which are thought to play a role in caveolae formation. It may also function as a scaffolding protein, which organizes signaling molecules. This functional role is supported by the fact that caveolin interacts directly with inactive ras and G-protein alpha subunits. Phosphorylation of caveolin at Tyr-14, Ser-88, and other residues in v-src transformed cells leads to flattening, aggregation, and fusion of caveolae and caveolae-derived vesicles. Thus, caveolin is the principle protein of caveolae and may be involved in v-src mediated cellular transformation.
This antibody has also been reported to cross-react to paxillin in mouse embryonic fibroblasts (MEF), observable to migrate at ~ 68 kDa .

Molecular Weight: 21-24 kDa