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NPAT antibody (AA 681-803)

This anti-NPAT antibody is a Mouse Monoclonal antibody detecting NPAT in WB and IF. Suitable for Human. This Primary Antibody has been cited in 1 publication.
Catalog No. ABIN968484

Quick Overview for NPAT antibody (AA 681-803) (ABIN968484)

Target

See all NPAT Antibodies
NPAT (Nuclear Protein, Ataxia-Telangiectasia Locus (NPAT))

Reactivity

  • 7
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Host

  • 5
  • 2
Mouse

Clonality

  • 5
  • 2
Monoclonal

Conjugate

  • 7
This NPAT antibody is un-conjugated

Application

  • 4
  • 4
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF)

Clone

27-NPAT
  • Binding Specificity

    • 2
    • 2
    AA 681-803

    Characteristics

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

    Purification

    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

    Immunogen

    Human NPAT aa. 681-803

    Isotype

    IgG2b
  • Comment

    Related Products: ABIN968537, ABIN967389

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    250 μg/mL

    Buffer

    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Store undiluted at -20° C.
  • Zhao, Dynlacht, Imai, Hori, Harlow: "Expression of NPAT, a novel substrate of cyclin E-CDK2, promotes S-phase entry." in: Genes & development, Vol. 12, Issue 4, pp. 456-61, (1998) (PubMed).

  • Target

    NPAT (Nuclear Protein, Ataxia-Telangiectasia Locus (NPAT))

    Alternative Name

    NPAT

    Background

    Cyclins regulate transitions between cell cycle phases by acting as regulatory subunits of the cyclin-dependent kinases (cdk). The temporal expression of cyclins is tightly regulated and plays a critical role in controlling the enzymatic activity of the cdks. Cyclin-dependent kinase 2 (Cdk2) is a member of a family of cdc2-related cell cycle protein kinases. Cdk2 is expressed earlier in the cell cycle than cdc2 and forms complexes with cyclins A, E, D1, and D3. It is not known if the D cyclins can form active complexes with Cdk2. Cyclin E-Cdk2 kinase is active in the G1 and S phases of the cell cycle and is important, as is Cyclin A-Cdk2, for the progression from G1 to S phase. One substrate for cyclin E-Cdk2 is a Nuclear Protein mapped to the Ataxia Telangiectasia locus, NPAT. This protein associates with cyclin E-Cdk2 and can be phosphorylated by Cdk2. NPAT protein levels peak at the G1/S boundary and overexpression of NPAT accelerates S phase entry, especially after coexpression of cyclin E-Cdk2. Thus, NPAT is a substrate of cyclin E-Cdk2 that may mediate G1 to S phase transition.
    Synonyms: Nuclear Protein Ataxia Telangiectasia

    Molecular Weight

    210 kDa
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