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IRS1 antibody (AA 1131-1234)

IRS1 Reactivity: Mouse, Rat WB, IF Host: Mouse Monoclonal 6-IRS unconjugated
Catalog No. ABIN968515
  • Target See all IRS1 Antibodies
    IRS1 (Insulin Receptor Substrate 1 (IRS1))
    Binding Specificity
    • 31
    • 28
    • 23
    • 21
    • 21
    • 19
    • 18
    • 17
    • 16
    • 16
    • 16
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1131-1234
    Reactivity
    • 292
    • 233
    • 209
    • 23
    • 19
    • 18
    • 17
    • 16
    • 16
    • 15
    • 2
    • 1
    • 1
    Mouse, Rat
    Host
    • 292
    • 12
    Mouse
    Clonality
    • 285
    • 19
    Monoclonal
    Conjugate
    • 138
    • 14
    • 12
    • 12
    • 12
    • 12
    • 12
    • 12
    • 11
    • 10
    • 10
    • 10
    • 10
    • 10
    • 10
    • 9
    This IRS1 antibody is un-conjugated
    Application
    • 172
    • 126
    • 104
    • 93
    • 92
    • 67
    • 58
    • 51
    • 24
    • 20
    • 13
    • 7
    • 4
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF)
    Cross-Reactivity
    Mouse (Murine)
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Rat IRS-1 aa. 1131-1234
    Clone
    6-IRS
    Isotype
    IgG1
    Top Product
    Discover our top product IRS1 Primary Antibody
  • Comment

    Related Products: ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20° C.
  • Obici, Feng, Karkanias, Baskin, Rossetti: "Decreasing hypothalamic insulin receptors causes hyperphagia and insulin resistance in rats." in: Nature neuroscience, Vol. 5, Issue 6, pp. 566-72, (2002) (PubMed).

    Paz, Liu, Shorer, Hemi, LeRoith, Quan, Kanety, Seger, Zick: "Phosphorylation of insulin receptor substrate-1 (IRS-1) by protein kinase B positively regulates IRS-1 function." in: The Journal of biological chemistry, Vol. 274, Issue 40, pp. 28816-22, (1999) (PubMed).

    Kuhné, Zhao, Rowles, Lavan, Shen, Fischer, Lienhard: "Dephosphorylation of insulin receptor substrate 1 by the tyrosine phosphatase PTP2C." in: The Journal of biological chemistry, Vol. 269, Issue 22, pp. 15833-7, (1994) (PubMed).

    Backer, Myers, Shoelson, Chin, Sun, Miralpeix, Hu, Margolis, Skolnik, Schlessinger: "Phosphatidylinositol 3'-kinase is activated by association with IRS-1 during insulin stimulation." in: The EMBO journal, Vol. 11, Issue 9, pp. 3469-79, (1992) (PubMed).

    Sun, Rothenberg, Kahn, Backer, Araki, Wilden, Cahill, Goldstein, White: "Structure of the insulin receptor substrate IRS-1 defines a unique signal transduction protein." in: Nature, Vol. 352, Issue 6330, pp. 73-7, (1991) (PubMed).

  • Target
    IRS1 (Insulin Receptor Substrate 1 (IRS1))
    Alternative Name
    IRS-1 (IRS1 Products)
    Synonyms
    HIRS-1 antibody, G972R antibody, IRS-1 antibody, IRS1IRM antibody, irs1 antibody, irsu antibody, IRS1 antibody, irs-1 antibody, irs1-a antibody, insulin receptor substrate 1 antibody, insulin receptor substrate 2 antibody, insulin receptor substrate 1 S homeolog antibody, IRS1 antibody, Irs1 antibody, irs2 antibody, irs1.S antibody
    Background
    The IRS (Insulin receptor substrate) proteins IRS-1, IRS-2, IRS-3, and IRS-4 are major substrates of the insulin receptor tyrosine kinase and the insulin-like growth factor-1 receptor. IRS proteins contain an N-terminal pleckstrin homology (PH) domain, an ATP-binding domain, and multiple tyrosine phosphorylation sites in the C-terminus. Following insulin receptor ligation, IRS-1 binds to the juxtamembrane region of the receptor and is tyrosine phosphorylated. This facilitates its interaction with SH2 domain-containing signaling proteins, such as PI3 kinase, fyn, Grb2, and PTP1D. Phosphorylation dramatically reduces the affinity of IRS-1 for the insulin receptor, indicating that dissociation from the receptor and subsequent subcellular translocation are important to IRS-1 function in the pleiotropic effects induced by insulin. In support of this, IRS-1-null mice are viable, but exhibit growth retardation and abnormal glucose metabolism. In cases of reduced IRS-1 expression, certain IRS-1 functions can be assumed by the related IRS-2 protein, while other activities linked to IRS-1 are inhibited. Thus, IRS-1 is an essential component of insulin induced signal transduction. This antibody is routinely tested by western blot analysis.
    Molecular Weight
    180 kDa
    Pathways
    Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process
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