IRS1 antibody (pSer307)

Catalog No. ABIN1531876

This Rabbit Polyclonal antibody specifically detects IRS1 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.It has been mentioned in 1 publication

Quick Overview for IRS1 antibody (pSer307) (ABIN1531876)

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-IRS1 Antibody

Binding Specificity: AA 274-323, pSer307

Purpose: IRS-1 (phospho Ser307) Antibody

Specificity: Phospho-IRS-1 (S307) Polyclonal Antibody detects endogenous levels of IRS-1 protein only when phosphorylated at S307.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human IRS-1 around the phosphorylation site of Ser307. AA range:274-323

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

References for anti-IRS1 antibody (ABIN1531876)

Simon-Szabó, Kokas, Mandl, Kéri, Csala: "Metformin attenuates palmitate-induced endoplasmic reticulum stress, serine phosphorylation of IRS-1 and apoptosis in rat insulinoma cells." in: PLoS ONE, Vol. 9, Issue 6, pp. e97868, (2014) (PubMed).

Target Details for IRS1

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Alternative Name: IRS-1

Background:

Synonyms: IRS1, Insulin receptor substrate 1, IRS-1

Background: This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009],

Gene Name: IRS1

Protein Name: Insulin receptor substrate 1

Molecular Weight: 131 kDa

Gene ID: 3667

OMIM: 125853

UniProt: P35568

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process