Solute Carrier Family 3 (Activators of Dibasic and Neutral Amino Acid Transport), Member 2 (SLC3A2) (AA 9-204) antibody

Antigen: Solute Carrier Family 3 (Activators of Dibasic and Neutral Amino Acid Transport), Member 2 (SLC3A2)

Synonyms: Mdu1, MGC53951, slc3a2, MGC53965, SLC3A2, MGC127541, MGC142823, DKFZp469L1516

Binding Site: AA 9-204

Clonality: Monoclonal (30)

Host: Mouse

Reactivity: Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968570

Additional Information

Alternative name: CD98HC

Immunogen: Rat 4F2 hc

Format: Liquid

Isotype: IgG1

Clone: 30

Description: 4F2 antigen (CD98) was identified as a heterodimer consisting of an 80 kDa type II glycosylated integral membrane protein 4F2 heavy chain (4F2 hc) linked by disulfide bonds to a hydrophobic, non-glycosylated, 37 kDa protein 4F2 light chain (4F2 lc). The 4F2 hc colocalizes with cadherins at cell adhesion sites and is required for the intracellular targeting of 4F2 heterodimers. 4F2 hc also associates with beta1 integrins and overexpression of 4F2 reverses the suppression of beta1 integrin activation caused by overexpression of beta1 cytoplasmic domains. 4F2 has also been identified as a fusion regulatory protein FRP-1, since anti-FRP-1 antibodies can induce HIV-mediated cell fusion via an integrin system. 4F2 heterodimers are also important for activation of amino acid transport. 4F2 hc is 30% homologous with the amino acid transport activator D2/rBAT and expression of 4F2 hc in Xenopus oocytes induces system y+L amino acid transport. Thus, 4F2 hc may participate in intracellular trafficking and activation of amino acid transporters, as well as in the regulation of integrin signaling.
Synonyms: 4F2 hc

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 80 kDa

Comments:

Related Products: ABIN967389, ABIN968548

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Restrictions: For Research Use only

Images

Western blot analysis of CD98HC on rat liver lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution on CD98HC.

Chick Fibroblast

anti-Solute Carrier Family 3 (Activators of Dibasic and Neutral Amino Acid Transport), Member 2 (SLC3A2) (AA 9-204) antibody (Image 3)

Publications

Product: Fenczik, Sethi, Ramos et al.: "Complementation of dominant suppression implicates CD98 in integrin activation." in: Nature, Vol. 390, Issue 6655, pp. 81-5, 1997 (PubMed).

Kanai, Segawa, Miyamoto et al.: "Expression cloning and characterization of a transporter for large neutral amino acids activated by the heavy chain of 4F2 antigen (CD98)." in: The Journal of biological chemistry, Vol. 273, Issue 37, pp. 23629-32, 1998 (PubMed).

Nakamura, Sato, Yang et al.: "4F2 (CD98) heavy chain is associated covalently with an amino acid transporter and controls intracellular trafficking and membrane topology of 4F2 heterodimer." in: The Journal of biological chemistry, Vol. 274, Issue 5, pp. 3009-16, 1999 (PubMed).

Tsurudome, Ito, Takebayashi et al.: "Cutting edge: primary structure of the light chain of fusion regulatory protein-1/CD98/4F2 predicts a protein with multiple transmembrane domains that is almost identical to the amino acid transporter E16." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 5, pp. 2462-6, 1999 (PubMed).