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STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (pTyr694)

Details for Product anti-STAT5A Antibody No. ABIN968702, Supplier: Login to see
Antigen
  • STAT5A
  • STAT5
  • MGF
  • AA959963
  • Stat5
  • STAT5A/MGF
  • STATA5
Alternatives
anti-Human STAT5A antibody for Immunohistochemistry (Frozen Sections)
Epitope
pTyr694
93
58
43
19
16
13
9
6
6
6
5
5
5
5
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3
3
3
3
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2
1
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1
1
1
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1
1
1
1
1
1
1
1
1
1
1
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1
1
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1
Reactivity
Human
421
203
161
28
21
17
13
11
6
1
1
Host
Mouse
340
98
10
Clonality (Clone)
Monoclonal ()
Conjugate
This STAT5A antibody is un-conjugated
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2
1
1
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Application
Western Blotting (WB)
340
200
135
63
57
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38
25
18
18
17
9
6
6
5
2
2
1
1
1
1
1
Supplier
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Immunogen Phosphorylated Human Phosphorylated Stat5 Peptide
Clone 47-Stat5
Isotype IgG1, kappa
Cross-Reactivity Human
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Stat5 (STAT5A Antibody Abstract)
Background Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat5 has been characterized and shown to be encoded by two separate genes, Stat5a and Stat5b that share over 90% identity at the amino acid level. Stat5a has been shown to be involved in lactogenesis and mammary development, while Stat5b has been shown to be involved in growth hormone signaling and to play a role in liver gene expression. Both Stat5a and Stat5b share similarities, both are involved in IL-2 induced peripheral T cell proliferation. The peptide hormone, prolactin, binds to the prolactin receptor (PRLR) to initiate the lactogenic response. There are at least three forms of PRLR, however, only the long form is able to activate the 92-kDa Stat5 protein by inducing phosphorylation at Y694. Once phosphorylated, Stat5 becomes an essential transcription factor which binds to the beta-casein gene promoter. The presence of an SH2 domain within Stat5 suggests that it may directly interact with protein tyrosine kinases (PTKs) such as JAK2.
The 47 monoclonal antibody recognizes the phosphorylated Y694 of Stat5a. The homologous phosphorylation site in Stat5b is Y699.
Molecular Weight 92 kDa
Research Area Signaling, Receptors, Inflammation
Pathways JAK-STAT Signaling, RTK Signaling
Application Notes Immunofluorescent staining and flow cytometry: Conjugated formats of this antibody are available and recommended.
Comment

Related Products: ABIN968534, ABIN967389, ABIN967810

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
 image for anti-STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (pTyr694) (ABIN968702) A431 cells were either left untreated (-) or treated (+) with 100 ng/ml EGF for 5 min...
 image for anti-STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (pTyr694) (ABIN968702) anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (pTyr694) antibody (Image 2)
 image for anti-STAT5A antibody (Signal Transducer and Activator of Transcription 5A) (pTyr694) (ABIN968702) anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (pTyr694) antibody (Image 3)
Product cited in: Williams, Cheung, Park et al.: "Loss of caveolin-1 gene expression accelerates the development of dysplastic mammary lesions in tumor-prone transgenic mice." in: Molecular biology of the cell, Vol. 14, Issue 3, pp. 1027-42, 2003 (PubMed).

Park, Lee, Frank et al.: "Caveolin-1-deficient mice show accelerated mammary gland development during pregnancy, premature lactation, and hyperactivation of the Jak-2/STAT5a signaling cascade." in: Molecular biology of the cell, Vol. 13, Issue 10, pp. 3416-30, 2002 (PubMed).

Gouilleux, Wakao, Mundt et al.: "Prolactin induces phosphorylation of Tyr694 of Stat5 (MGF), a prerequisite for DNA binding and induction of transcription." in: The EMBO journal, Vol. 13, Issue 18, pp. 4361-9, 1994 (PubMed).

Gouilleux, Pallard, Dusanter-Fourt et al.: "Prolactin, growth hormone, erythropoietin and granulocyte-macrophage colony stimulating factor induce MGF-Stat5 DNA binding activity." in: The EMBO journal, Vol. 14, Issue 9, pp. 2005-13, 1995 (PubMed).

Wakao, Gouilleux, Groner: "Mammary gland factor (MGF) is a novel member of the cytokine regulated transcription factor gene family and confers the prolactin response." in: The EMBO journal, Vol. 13, Issue 9, pp. 2182-91, 1994 (PubMed).