HIP1R antibody (AA 560-772)

Catalog No. ABIN968746

This Mouse Monoclonal antibody specifically detects HIP1R in WB and IF. It exhibits reactivity toward Human, Rat, Mouse and Dog and has been mentioned in 3+ publications.

Quick Overview for HIP1R antibody (AA 560-772) (ABIN968746)

Target: HIP1R (Huntingtin Interacting Protein 1 Related (HIP1R))

Reactivity: Human, Rat, Mouse, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This HIP1R antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 44-Hip1R

Product Details anti-HIP1R Antibody

Binding Specificity: AA 560-772

Cross-Reactivity: Human, Dog (Canine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Hip1R aa. 560-772

Isotype: IgG1

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-HIP1R antibody (ABIN968746)

Itoh, Koshiba, Kigawa, Kikuchi, Yokoyama, Takenawa: "Role of the ENTH domain in phosphatidylinositol-4,5-bisphosphate binding and endocytosis." in: Science (New York, N.Y.), Vol. 291, Issue 5506, pp. 1047-51, (2001) (PubMed).

Engqvist-Goldstein, Kessels, Chopra, Hayden, Drubin: "An actin-binding protein of the Sla2/Huntingtin interacting protein 1 family is a novel component of clathrin-coated pits and vesicles." in: The Journal of cell biology, Vol. 147, Issue 7, pp. 1503-18, (2000) (PubMed).

Seki, Muramatsu, Sugano, Suzuki, Nakagawara, Ohhira, Hayashi, Hori, Saito: "Cloning, expression analysis, and chromosomal localization of HIP1R, an isolog of huntingtin interacting protein (HIP1)." in: Journal of human genetics, Vol. 43, Issue 4, pp. 268-71, (1999) (PubMed).

Target Details for HIP1R

Target: HIP1R (Huntingtin Interacting Protein 1 Related (HIP1R))

Alternative Name: Hip1R

Background: Clathrin is the major protein component in the coat formed around pits and vesicles involved in receptor-mediated endocytosis. Clathrin forms a non-covalently bound triskelion structure composed of three heavy chains (192kDa each) and three light chains (23-25kDa each). A variety of proteins facilitate receptor-mediated endocytosis through association with clathrin-coated vesicles. Huntingtin interacting protein 1 (Hip1) is an actin-binding protein that interacts with Huntingtin protein, and has been implicated in vesicular transport defects found in Huntingtin's disease. Hip1 related protein (Hip1R) is another actin binding protein that contains an epsin NH2-terminal homology (ENTH)domain, three coiled-coil regions, a leucine zipper, and a talin-like actin binding domain. Hip1R mRNA is widely expressed, and Hip1R protein is enriched in the cell cortex and perinuclear region. The ENTH domain of Hip1R is required for binding to phosphatidylinositol-4,5-bisphosphate, and this complex is essential for clathrin-mediated endocytosis. In addition, Hip1R colocalizes with clathrin, AP-2 and endocytosed transferrin. Thus, Hip1R may facilitate interactions between clathrin-coated pits and actin during endocytosis.

Molecular Weight: 120 kDa