Lamin A/C antibody (AA 398-490)

Catalog No. ABIN968766

Product Details anti-Lamin A/C Antibody

Target: Lamin A/C (LMNA)

Binding Specificity: AA 398-490

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Lamin A/C antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Human, Chicken, Dog (Canine), Mouse (Murine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Lamin A/C aa. 398-490

Clone: 14-LaminAC

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-Lamin A/C antibody (ABIN968766)

Shackleton, Lloyd, Jackson, Evans, Niermeijer, Singh, Schmidt, Brabant, Kumar, Durrington, Gregory, ORahilly, Trembath: "LMNA, encoding lamin A/C, is mutated in partial lipodystrophy." in: Nature genetics, Vol. 24, Issue 2, pp. 153-6, (2000) (PubMed).

Sullivan, Escalante-Alcalde, Bhatt, Anver, Bhat, Nagashima, Stewart, Burke: "Loss of A-type lamin expression compromises nuclear envelope integrity leading to muscular dystrophy." in: The Journal of cell biology, Vol. 147, Issue 5, pp. 913-20, (1999) (PubMed).

McKeon, Kirschner, Caput: "Homologies in both primary and secondary structure between nuclear envelope and intermediate filament proteins." in: Nature, Vol. 319, Issue 6053, pp. 463-8, (1986) (PubMed).

Target Details for Lamin A/C

Target: Lamin A/C (LMNA)

Alternative Name: Lamin A/C (LMNA Products)

Background: The nuclear envelope (NE) is a specialized extension of the ER that contains numerous pore complexes interconnected with the nuclear lamina. The nuclear lamina composes the structural framework for the NE and serves as a chromatin anchor site, thus playing a major role in interphase nuclear organization. The major component of nuclear lamina are intermediate filament-like proteins called lamins. In mammalian somatic cells, there are three major lamins, A, B1, and C, and two minor lamins, B2 and A10. A-type lamins (A, A10, and C) are encoded by a single gene and are produced by alternative splicing, while B-type lamins (B1 and B2) are encoded by separate genes. B-type lamins are found in all nucleated somatic cells, while the expression of A-type lamins are developmentally regulated. Mice lacking lamin A show no overt abnormalities until postnatal development when perturbations in nuclear envelop structure correlate with the appearance of muscular dystrophy. In addition, lamin A is mutated in lipodystrophy, a disorder characterized by reduction in subcutaneous adipose tissue. Thus, lamin A and C may be important for nuclear envelope formation during postnatal cell differentiation. This antibody is routinely tested by western blot analysis.

Molecular Weight: 65 & 74 kDa

Pathways: Apoptosis, Caspase Cascade in Apoptosis, ER-Nucleus Signaling, Protein targeting to Nucleus