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PPP1R12A antibody (AA 723-840)

PPP1R12A Reactivity: Human, Mouse, Rat WB Host: Mouse Monoclonal 20-MYPT1 unconjugated
Catalog No. ABIN968768
  • Target See all PPP1R12A Antibodies
    PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))
    Binding Specificity
    • 27
    • 23
    • 7
    • 7
    • 7
    • 7
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 723-840
    Reactivity
    • 93
    • 80
    • 67
    • 6
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    Human, Mouse, Rat
    Host
    • 111
    • 1
    • 1
    Mouse
    Clonality
    • 112
    • 1
    Monoclonal
    Conjugate
    • 50
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This PPP1R12A antibody is un-conjugated
    Application
    • 58
    • 26
    • 26
    • 26
    • 17
    • 13
    • 7
    • 6
    • 4
    • 2
    • 1
    • 1
    Western Blotting (WB)
    Cross-Reactivity
    Human, Mouse (Murine)
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Rat MYPT1 aa. 723-840
    Clone
    20-MYPT1
    Isotype
    IgG1
    Top Product
    Discover our top product PPP1R12A Primary Antibody
  • Comment

    Related Products: ABIN968545, ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20°C.
  • Khatri, Joyce, Brozovich, Fisher: "Role of myosin phosphatase isoforms in cGMP-mediated smooth muscle relaxation." in: The Journal of biological chemistry, Vol. 276, Issue 40, pp. 37250-7, (2001) (PubMed).

    Kimura, Ito, Amano, Chihara, Fukata, Nakafuku, Yamamori, Feng, Nakano, Okawa, Iwamatsu, Kaibuchi: "Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase)" in: Science (New York, N.Y.), Vol. 273, Issue 5272, pp. 245-8, (1996) (PubMed).

    Chen, Chen, Alessi, Campbell, Shanahan, Cohen, Cohen: "Molecular cloning of cDNA encoding the 110 kDa and 21 kDa regulatory subunits of smooth muscle protein phosphatase 1M." in: FEBS letters, Vol. 356, Issue 1, pp. 51-5, (1995) (PubMed).

  • Target
    PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))
    Alternative Name
    MYPT1 (PPP1R12A Products)
    Synonyms
    1200015F06Rik antibody, 5730577I22Rik antibody, AA792106 antibody, AV099298 antibody, D10Ertd625e antibody, Mypt1 antibody, M130 antibody, MBS antibody, MYPT1 antibody, M110 antibody, MBSP antibody, protein phosphatase 1, regulatory (inhibitor) subunit 12A antibody, protein phosphatase 1 regulatory subunit 12A antibody, protein phosphatase 1, regulatory subunit 12A antibody, Ppp1r12a antibody, PPP1R12A antibody
    Background
    Protein phosphatase-1 (PP1) is a major serine/threonine phosphatase that is involved in various cellular processes in eukaryotic cells. The PP1 catalytic subunit may be directed to particular subcellular locations by targeting subunits, which can modify the substrate specificity of the enzyme. The PP1 enzyme associated with the myofibrils of striated and smooth muscle forms a complex with Myosin Phosphatase Targeting subunit 1 (MYPT1). MYPT1 contains seven ankyrin repeats in the N-terminal region, and a leucine zipper (LZ) motif in the C-terminus. Alternative splicing of the MYPT1 mRNA leads to expression of a truncated MYPT1 that lacks the leucine zipper region. The expression of MYPT1 splice variants correlate with a smooth muscle phenotype. In the tonic contracting chicken aorta, only the full length MYPT1 is expressed, while in the phasic contracting chicken gizzard, only the truncated MYPT1 is expressed. The leucine zipper motif may be required for association with cGMP-dependent protein kinase I. Thus, MYPT1 may direct not only PP1 enzymatic activity toward myosin, but also may regulate the cGMP responsiveness of PP1 through expression of alternative splice variants.
    Synonyms: Myosin Phosphatase Targeting subunit 1
    Molecular Weight
    130 kDa
    Pathways
    M Phase
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