Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (AA 723-840) antibody

Details for Product No. ABIN968768
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Antigen
Synonyms 1200015F06Rik, 5730577I22Rik, AA792106, AV099298, D10Ertd625e, Mypt1, M130, MBS, MYPT1, M110, MBSP
Epitope
AA 723-840
(20), (12), (12), (12), (11), (9), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Rat (Rattus)
(116), (88), (88), (60), (49), (48), (1)
Host
Mouse
(112), (3), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application
Western Blotting (WB)
(61), (50), (33), (31), (20), (6), (3), (2), (1)
Pubmed 3 references available
Quantity 150 μg
Options
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Catalog No. ABIN968768
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Immunogen Rat MYPT1
Clone LEP-02
Isotype IgG1
Cross-Reactivity Human, Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name MYPT1
Background Protein phosphatase-1 (PP1) is a major serine/threonine phosphatase that is involved in various cellular processes in eukaryotic cells. The PP1 catalytic subunit may be directed to particular subcellular locations by targeting subunits, which can modify the substrate specificity of the enzyme. The PP1 enzyme associated with the myofibrils of striated and smooth muscle forms a complex with Myosin Phosphatase Targeting subunit 1 (MYPT1). MYPT1 contains seven ankyrin repeats in the N-terminal region, and a leucine zipper (LZ) motif in the C-terminus. Alternative splicing of the MYPT1 mRNA leads to expression of a truncated MYPT1 that lacks the leucine zipper region. The expression of MYPT1 splice variants correlate with a smooth muscle phenotype. In the tonic contracting chicken aorta, only the full length MYPT1 is expressed, while in the phasic contracting chicken gizzard, only the truncated MYPT1 is expressed. The leucine zipper motif may be required for association with cGMP-dependent protein kinase I. Thus, MYPT1 may direct not only PP1 enzymatic activity toward myosin, but also may regulate the cGMP responsiveness of PP1 through expression of alternative splice variants.
Synonyms: Myosin Phosphatase Targeting subunit 1
Molecular Weight 130 kDa
Comment

Related Products: ABIN968545, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (AA 723-840) antibody Western blot analysis of MYPT1 on a rat cerebrum lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-MYPT1 antibody.
Product cited in: Chen, Chen, Alessi et al.: "Molecular cloning of cDNA encoding the 110 kDa and 21 kDa regulatory subunits of smooth muscle protein phosphatase 1M." in: FEBS letters, Vol. 356, Issue 1, pp. 51-5, 1995 (PubMed).

Kimura, Ito, Amano et al.: "Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase)" in: Science (New York, N.Y.), Vol. 273, Issue 5272, pp. 245-8, 1996 (PubMed).

Khatri, Joyce, Brozovich et al.: "Role of myosin phosphatase isoforms in cGMP-mediated smooth muscle relaxation." in: The Journal of biological chemistry, Vol. 276, Issue 40, pp. 37250-7, 2001 (PubMed).

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