PPP1R12A antibody (Myosin Phosphatase, Target Subunit 1) (C-Term)

Details for Product anti-PPP1R12A Antibody No. ABIN6391349
Antigen
Epitope
C-Term
33
25
15
13
9
8
8
8
4
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
174
138
117
10
9
9
5
5
3
3
3
3
2
1
Host
Goat
173
1
Clonality
Polyclonal
Conjugate
This PPP1R12A antibody is un-conjugated
9
9
9
9
9
9
9
2
2
2
2
2
2
2
1
Application
ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
107
52
51
28
21
8
7
2
2
2
1
1
Options
Purpose PPP1R12A
Sequence REDEYKQKYS RTYD
Isotype IgG
Predicted Reactivity Human
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Antigen
Alternative Name PPP1R12A (PPP1R12A Antibody Abstract)
Background Protein phosphatase 1, regulatory (inhibitor) subunit 12A, MBS, MGC133042, MYPT1, myosin phosphatase, target subunit 1
NCBI Accession NP_002471, NP_001231919, NP_001231921, NP_001137358
Pathways M Phase
Application Notes

DS WB Results: Preliminary experiments gave an approx 150 kDa band in lysates of cell line MCF7 at 0.3-0.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for this band, given the calculated size of 115 kDa according to NP_002471.1. The 150 kDa band was successfully blocked by incubation with the immunizing peptide.

DS IHC Results: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 3.75 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Supplier Images
Image no. 1 for anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (C-Term) antibody (ABIN6391349) ABIN6391349 (3.75µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed ...
Image no. 2 for anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (C-Term) antibody (ABIN6391349) ABIN6391349 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue lin...
Image no. 3 for anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (C-Term) antibody (ABIN6391349) ABIN6391349 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeab...
Image no. 4 for anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (C-Term) antibody (ABIN6391349) ABIN6391349 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeab...