Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) (AA 1093-1214) antibody

Antigen: Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A)

Synonyms: GRIN2A, NMDAR2A, NR2A, nr2a

Binding Site: AA 1093-1214

Clonality: Monoclonal (5)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN968802

Additional Information

Alternative name: NMDAR2A

Immunogen: Mouse NMDAR2A

Cross-Reactivity: Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 5

Description: The majority of synapses in the central nervous system utilize glutamate as a neurotransmitter to produce rapid neuronal excitation. Glutamate has a diverse array of receptors that can be categorized into two groups, ionotropic and metabotropic. The ionotropic receptors are subdivided into two distinct types: 1) receptors for N-methyl D-aspartate (NMDAR) and 2) non-NMDA receptors for AMPA and kainate. Four types of NMDAR2 have been identified: NMDAR2A (NR2A), NR2B, NR2C, and NR2D. NR2A and NR2B contain a C-terminal extension (> 600 amino acids) that has small, scattered regions of conserved sequence. In embryonic stages, NR2B is expressed throughout the brain, while NR2D is found in diencephalon and brainstem. After birth, NR2A is found throughout the brain, and NR2C is expressed in cerebellum. At this time, NR2B expression becomes restricted to the forebrain, and NR2D expression is strongly reduced. Deletion of the most widespread postnatal NR subunit, NR2A, does not have adverse affects on brain development, but reduces long-term potentiation (LTP) in the hippocampus and impairs spatial and contextual learning. Thus, NR2A may be critical for postnatal learning. This antibody is routinely tested by western blot analysis.
Synonyms: N-Methyl D-Aspartate

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 180 kDa

Comments:

Related Products: ABIN968540, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Neurology

Restrictions: For Research Use only

Images

Western blot analysis of NMDAR2A on a mouse cerebrum lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the anti- NMDAR2A antibody.

Publications

Product: Ishii, Moriyoshi, Sugihara et al.: "Molecular characterization of the family of the N-methyl-D-aspartate receptor subunits." in: The Journal of biological chemistry, Vol. 268, Issue 4, pp. 2836-43, 1993 (PubMed).

Morikawa, Mori, Kiyama et al.: "Attenuation of focal ischemic brain injury in mice deficient in the epsilon1 (NR2A) subunit of NMDA receptor." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 18, Issue 23, pp. 9727-32, 1998 (PubMed).