Lymphocyte-Specific Protein tyrosine Kinase (LCK) (pTyr505) antibody

Antigen: Lymphocyte-Specific Protein tyrosine Kinase (LCK)

Synonyms: YT16, p56lck, pp58lck, Lck1, Lcktkr, MGC136695, MGC194004, MGC194043, zgc:136695, LCK, MGC126900

Binding Site: pTyr505

Clonality: Monoclonal (4)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Catalog no.: ABIN968844

Additional Information

Alternative name: Lck

Immunogen: Human Lck (pY505)

Cross-Reactivity: Rat (Rattus), Mouse (Murine)

Format: Liquid

Isotype: IgG1

Clone: 4

Description: Protein tyrosine phosphorylation is an essential step in the signal transduction cascade leading to T cell antigen receptor (TCR) activation. Lck is a protein kinase and a member of the src family of cytoplasmic protein-tyrosine kinases (PTKs). Members of this family have several common features: 1) unique N-terminal domains, 2) attachment to cellular membranes through a myristylated N-terminus, and 3) homologous SH2, SH3, and catalytic domains. The unique N-terminal domain of Lck interacts with the cytoplasmic tails of the CD4 and CD8 cell surface glycoproteins. CD4 and CD8 bind to surface MHC class II and class I molecules, respectively. Lck is regulated by both kinases and phosphatases. Autophosphorylation at Y394 leads to conformational changes in the catalytic domain, which induces kinase activity. Repression of Lck occurs via phosphorylation at Y505, located near the carboxy-terminus. Phosphorylation of this tyrosine site is mediated by the Csk family of PTKs. Upon phosphorylation at this site, Lck associates with the SH2 domain in the amino-terminus, thus keeping the protein biologically inactive. Lck activity and regulation is critical for activation and development of T cells.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 56 kDa

Comments:

Related Products: ABIN968537, ABIN968656, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Immunology, Tyrosine Kinases, Adaptive Immunity, CD Antigens, Signaling

Restrictions: For Research Use only

Images

Jurkat cells were either untreated (lane 1) or treated (lane 2) with Anti-CD3 for 15 minutes at 37°C. The top panel was probed with Lck (ABIN967742) and the bottom panel was probed with Lck (pY505) (ABIN968843).

Publications

Product: Lee-Fruman, Collins, Burakoff: "Role of the Lck Src homology 2 and 3 domains in protein tyrosine phosphorylation." in: The Journal of biological chemistry, Vol. 271, Issue 40, pp. 25003-10, 1996 (PubMed).

Hardwick, Sefton: "The activated form of the Lck tyrosine protein kinase in cells exposed to hydrogen peroxide is phosphorylated at both Tyr-394 and Tyr-505." in: The Journal of biological chemistry, Vol. 272, Issue 41, pp. 25429-32, 1997 (PubMed).

Wang, Lemay, Tsai et al.: "SH2 domain-mediated interaction of inhibitory protein tyrosine kinase Csk with protein tyrosine phosphatase-HSCF." in: Molecular and cellular biology, Vol. 21, Issue 4, pp. 1077-88, 2001 (PubMed).