LCK antibody (Lymphocyte-Specific Protein tyrosine Kinase) (AA 22-36)

Details for Product anti-LCK Antibody No. ABIN94422
Antigen
Epitope
AA 22-36
57
24
23
15
15
14
12
11
11
8
8
7
7
5
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
380
221
170
11
10
8
7
4
4
4
3
2
1
Host
Mouse
315
62
9
Clonality (Clone)
Monoclonal ()
Conjugate
This LCK antibody is un-conjugated
14
10
9
6
6
6
6
6
6
6
6
5
2
2
2
2
2
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
326
163
82
53
47
34
33
26
21
12
4
4
3
2
1
1
Options
Immunogen Peptide corresponding to amino acids 22-36 in the sequence of human Lck.
Clone LCK-01
Isotype IgG1
Specificity The antibody LCK-01 recognizes defined epitope (aa 22-36) of Lck, a 56 kDa Src-family protein tyrosine kinase.
No Cross-Reactivity Mouse (Murine)
Cross-Reactivity (Details) Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name Lck (LCK Antibody Abstract)
Background Lck is a Src-family tyrosine kinase, which is essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, Lck phosphorylates the ITAM motives in its zeta subunits, establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by Lck and thereby activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. Whereas the majority of Lck is localized to the plasma membrane, there is also a significant fraction associated with the Golgi apparatus, which may contribute to Raf activation under conditions of weak stimulation through the TCR. Lck is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors.
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (AA 22-36) antibody (ABIN94422) Western Blotting analysis (reducing conditions) of human Lck in whole cell lysate usi...
Product cited in: Stepanek, Kalina, Draber, Skopcova, Svojgr, Angelisova, Horejsi, Weiss, Brdicka: "Regulation of Src family kinases involved in T cell receptor signaling by protein-tyrosine phosphatase CD148." in: The Journal of biological chemistry, Vol. 286, Issue 25, pp. 22101-12, 2011 (PubMed).

Hrdinka, Dráber, Stepánek, Ormsby, Otáhal, Angelisová, Brdicka, Paces, Horejsí, Drbal: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, 2011 (PubMed).

Ota: "The effects of membrane compartmentalization of Csk on TCR signaling." in: Biochimica et biophysica acta, 2010 (PubMed).

Meraner, Horejsí, Wolpl, Fischer, Stingl, Maurer: "Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 4, pp. 2262-71, 2007 (PubMed).

Brdicková, Brdicka, Angelisová, Horváth, Spicka, Hilgert, Paces, Simeoni, Kliche, Merten, Schraven, Horejsí: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003 (PubMed).

Romagnoli, Strahan, Pelosi, Cantagrel, van Meerwijk: "A potential role for protein tyrosine kinase p56(lck) in rheumatoid arthritis synovial fluid T lymphocyte hyporesponsiveness." in: International immunology, Vol. 13, Issue 3, pp. 305-12, 2001 (PubMed).

Cebecauer, Cerný, Horejsí: "Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells." in: Biochemical and biophysical research communications, Vol. 243, Issue 3, pp. 706-10, 1998 (PubMed).