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Details for Product No. ABIN969067

CREB Regulated Transcription Coactivator 2 (CRTC2) antibody

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Antigen
Synonyms TORC-2, TORC2, Torc2, mTORC2, 4632407F12Rik, RGD1308903, CRTC2
Reactivity
»Alternatives Human, Monkey
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Fluorescence Microscopy (FM)
Pubmed 3 references available
Catalog no. ABIN969067
Quantity 100 µL
Price
363.00 $   Plus shipping costs $45.00
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Immunogen Purified recombinant fragment of human CRTC2 expressed in E. coli.
Clone 5B10
Isotype IgG1
Purification Ascites
Alternative Name CRTC2
Background Synonyms:
TORC2, TORC-2, CRTC2
Glucose homeostasis is regulated by hormones and cellular energy status. Elevations of blood glucose during feeding stimulate insulin release from pancreatic ß-cells through a glucose sensing pathway. Feeding also stimulates release of gut hormones such as glucagon-like peptide-1 (GLP-1), which further induces insulin release, inhibits glucagon release and promotes ß-cell viability. CREB-dependent transcription likely plays a role in both glucose sensing and GLP-1 signaling. The protein Torc2 (transducer of regulated CREB activity 2) functions as a CREB co-activator and is implicated in mediating the effects of these two pathways. In quiescent cells, Torc2 is phosphorylated at Ser171 and becomes sequestered in the cytoplasm via an interaction with 14-3-3 proteins. Glucose and gut hormones lead to the dephosphorylation of Torc2 and its dissociation from 14-3-3 proteins. Dephosphorylated Torc2 enters the nucleus to promote CREB-dependent transcription. Torc2 plays a key role in the regulation of hepatic gluconeogenic gene transcription in response to hormonal and energy signals during fasting. Tissue specificity: Most abundantly expressed in the thymus. Present in both B and T lymphocytes. Highly expressed in HEK293T cells and in insulinomas. High levels also in spleen, ovary, muscle and lung, with highest levels in muscle. Lower levels found in brain, colon, heart, kidney, prostate, small intestine and stomach. Weak expression in liver and pancreas.
Molecular Weight 80 kDa
Gene ID 200186
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Product cited in: Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).

Dentin, Liu, Koo et al.: "Insulin modulates gluconeogenesis by inhibition of the coactivator TORC2." in: Nature, Vol. 449, Issue 7160, pp. 366-9, 2007 (PubMed).

Murata, Sato, Nakayama et al.: "TORC2, a coactivator of cAMP-response element-binding protein, promotes Epstein-Barr virus reactivation from latency through interaction with viral BZLF1 protein." in: The Journal of biological chemistry, Vol. 284, Issue 12, pp. 8033-41, 2009 (PubMed).

Alternatives for antigen "CREB Regulated Transcription Coactivator 2 (CRTC2)", type "Antibodies"
Hosts (42), (10), (8), (1)
Reactivities (57), (33), (33), (27), (27), (15), (6), (2), (2), (2), (1), (1), (1), (1)
Applications (42), (28), (20), (11), (10), (7), (4), (3), (3)
Conjugates (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (12), (7), (6), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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