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CRTC2 antibody (CREB Regulated Transcription Coactivator 2)

Details for Product anti-CRTC2 Antibody No. ABIN969067, Supplier: Login to see New
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Synonyms CRTC2, TORC-2, TORC2, 4632407F12Rik, Torc2, mTORC2, RGD1308903
Human, Monkey
(127), (53), (37), (7), (7), (7), (4), (2), (2), (2)
(80), (55), (14), (2)
Clonality (Clone)
Monoclonal ()
This CRTC2 antibody is un-conjugated
(10), (10), (10), (9), (9), (9), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(133), (99), (28), (17), (13), (13), (6), (4), (3), (3)
Pubmed 3 references available
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Quantity 100 μL
Shipping to United States ( )
Availability Will be delivered in 3 to 4 Business Days
Immunogen Purified recombinant fragment of human CRTC2 expressed in E. coli.
Clone 5B10
Isotype IgG1
Purification Ascites
Alternative Name CRTC2 (CRTC2 Antibody Abstract)
Background Glucose homeostasis is regulated by hormones and cellular energy status. Elevations of blood glucose during feeding stimulate insulin release from pancreatic β-cells through a glucose sensing pathway. Feeding also stimulates release of gut hormones such as glucagon-like peptide-1 (GLP-1), which further induces insulin release, inhibits glucagon release and promotes β-cell viability. CREB-dependent transcription likely plays a role in both glucose sensing and GLP-1 signaling . The protein Torc2 (transducer of regulated CREB activity 2) functions as a CREB co-activator and is implicated in mediating the effects of these two pathways . In quiescent cells, Torc2 is phosphorylated at Ser171 and becomes sequestered in the cytoplasm via an interaction with 14-3-3 proteins. Glucose and gut hormones lead to the dephosphorylation of Torc2 and its dissociation from 14-3-3 proteins. Dephosphorylated Torc2 enters the nucleus to promote CREB-dependent transcription. Torc2 plays a key role in the regulation of hepatic gluconeogenic gene transcription in response to hormonal and energy signals during fasting.Tissue specificity: Most abundantly expressed in the thymus. Present in both B and T lymphocytes. Highly expressed in HEK293T cells and in insulinomas. High levels also in spleen, ovary, muscle and lung, with highest levels in muscle. Lower levels found in brain, colon, heart, kidney, prostate, small intestine and stomach. Weak expression in liver and pancreas .
Molecular Weight 80 kDa
Gene ID 200186
HGNC 200186
Pathways AMPK Signaling
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
Flow Cytometry (FACS) image for anti-CRTC2 antibody (CREB Regulated Transcription Coactivator 2) (ABIN969067) Figure 1: Flow cytometric analysis of Hela cells using CRTC2 mouse mAb (green) and ne...
Immunocytochemistry (ICC) image for anti-CRTC2 antibody (CREB Regulated Transcription Coactivator 2) (ABIN969067) Figure 2: Immunofluorescence analysis of Hela cells using CRTC2 mouse mAb (green) . B...
Immunohistochemistry (IHC) image for anti-CRTC2 antibody (CREB Regulated Transcription Coactivator 2) (ABIN969067) Figure 3: Immunohistochemical analysis of paraffin-embedded ovary tumour tissues (lef...
Western Blotting (WB) image for anti-CRTC2 antibody (CREB Regulated Transcription Coactivator 2) (ABIN969067) Figure 4: Western blot analysis using CRTC2 mouse mAb against Hela (1) and HEK293 (2)...
Product cited in: Murata, Sato, Nakayama et al.: "TORC2, a coactivator of cAMP-response element-binding protein, promotes Epstein-Barr virus reactivation from latency through interaction with viral BZLF1 protein." in: The Journal of biological chemistry, Vol. 284, Issue 12, pp. 8033-41, 2009 (PubMed).

Dentin, Liu, Koo et al.: "Insulin modulates gluconeogenesis by inhibition of the coactivator TORC2." in: Nature, Vol. 449, Issue 7160, pp. 366-9, 2007 (PubMed).

Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: , Vol. 3, pp. 89, 2007 (PubMed).

Catalog No. ABIN969067
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