CREB Regulated Transcription Coactivator 2 (CRTC2) antibody

Antigen: CREB Regulated Transcription Coactivator 2 (CRTC2)

Synonyms: TORC2, RP11-422P24.6, Torc2, mTORC2, 4632407F12Rik, RGD1308903, CRTC2, MGC139531

Clonality: Monoclonal (5B10)

Host: Mouse

Reactivity: Human, Monkey

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB)

Catalog no.: ABIN969067

Additional Information

Alternative name: CRTC2

Gene ID: 200186

Immunogen: Purified recombinant fragment of human CRTC2 expressed in E. Coli.

Format: Liquid

Isotype: IgG1

Clone: 5B10

Description: Glucose homeostasis is regulated by hormones and cellular energy status. Elevations of blood glucose during feeding stimulate insulin release from pancreatic beta-cells through a glucose sensing pathway. Feeding also stimulates release of gut hormones such as glucagon-like peptide-1 (GLP-1), which further induces insulin release, inhibits glucagon release and promotes beta-cell viability. CREB-dependent transcription likely plays a role in both glucose sensing and GLP-1 signaling . The protein Torc2 (transducer of regulated CREB activity 2) functions as a CREB co-activator and is implicated in mediating the effects of these two pathways . In quiescent cells, Torc2 is phosphorylated at Ser171 and becomes sequestered in the cytoplasm via an interaction with 14-3-3 proteins. Glucose and gut hormones lead to the dephosphorylation of Torc2 and its dissociation from 14-3-3 proteins. Dephosphorylated Torc2 enters the nucleus to promote CREB-dependent transcription. Torc2 plays a key role in the regulation of hepatic gluconeogenic gene transcription in response to hormonal and energy signals during fasting.Tissue specificity: Most abundantly expressed in the thymus. Present in both B and T lymphocytes. Highly expressed in HEK293T cells and in insulinomas. High levels also in spleen, ovary, muscle and lung, with highest levels in muscle. Lower levels found in brain, colon, heart, kidney, prostate, small intestine and stomach. Weak expression in liver and pancreas .
Synonyms: TORC2, TORC-2, CRTC2

Molecular Weight: 80kDa

Application Details

Application Notes: Western Blotting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Purification: Ascites

Buffer: Ascitic fluid containing 0.03% sodium azide.

Preservative: 0.03% sodium azide

Storage: Store at 4℃, for long term storage, store at -20℃.

Restrictions: For Research Use only

Publications

Product: Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).

Dentin, Liu, Koo et al.: "Insulin modulates gluconeogenesis by inhibition of the coactivator TORC2." in: Nature, Vol. 449, Issue 7160, pp. 366-9, 2007 (PubMed).

Murata, Sato, Nakayama et al.: "TORC2, a coactivator of cAMP-response element-binding protein, promotes Epstein-Barr virus reactivation from latency through interaction with viral BZLF1 protein." in: The Journal of biological chemistry, Vol. 284, Issue 12, pp. 8033-41, 2009 (PubMed).