Nerve Growth Factor Receptor (NGFR) antibody

Antigen: Nerve Growth Factor Receptor (NGFR)

Synonyms: CD271, p75NTR, TNFRSF16, p75(NTR), Gp80-LNGFR, MGC148470, NGFR, wu:fb38c02, wu:fc10h01, p75, LNGFR, RNNGFRR, nrh1, NRH1b, p75ntr, tnfrsf16, MGC131034

Clonality: Monoclonal (2F1C2)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB)

Catalog no.: ABIN969315

Additional Information

Alternative name: NGFR

Gene ID: 4804

Immunogen: Purified recombinant fragment of human NGFR expressed in E. Coli.

Format: Liquid

Isotype: IgG1

Clone: 2F1C2

Description: Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGFR p75 plays a central role in the regulation of cell number by apoptosis in the developing CNS. During early development, activation of NGFR p75 by NGF induces apoptotic cell death in some neuronal cells, probably through activation of the sphingomyelinase/ceramide pathway, the ICE like proteases and the JNK pathway. In rat Schwann cells, NGF binding to NGFR p75 activates NF kappaB, possibly to modulate Schwann cell migration during nerve regeneration. CD271 has recently been described as being expressed in mesenchymal stem cells (bone marrow stromal cells).
Synonyms: CD271, p75NTR, TNFRSF16, p75(NTR), Gp80-LNGFR, NGFR

Molecular Weight: 45kDa

Application Details

Application Notes: Western Blotting: 1/500 - 1/2000.
Immunofluorescence: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Purification: Ascites

Buffer: Ascitic fluid containing 0.03% sodium azide.

Preservative: 0.03% sodium azide

Storage: Store at 4℃, for long term storage, store at -20℃.

Research Area: Growth Factors, Receptors

Restrictions: For Research Use only

Publications

Product: DiDonato, Hayakawa, Rothwarf et al.: "A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB." in: Nature, Vol. 388, Issue 6642, pp. 548-54, 1997 (PubMed).

Hu, Zhang, Qin et al.: "Increased p75(NTR) expression in hippocampal neurons containing hyperphosphorylated tau in Alzheimer patients." in: Experimental neurology, Vol. 178, Issue 1, pp. 104-11, 2002 (PubMed).

He, Garcia: "Structure of nerve growth factor complexed with the shared neurotrophin receptor p75." in: Science (New York, N.Y.), Vol. 304, Issue 5672, pp. 870-5, 2004 (PubMed).