Sirtuin 1 (SIRT1) antibody

Details for Product No. ABIN969396
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Antigen
Synonyms sirtuin 1, AA673258, SIR2L1, Sir2, Sir2a, Sir2alpha, sir2l1, sirtuin, SIR2
Reactivity
Human, Monkey
(156), (89), (43), (27), (25), (14), (13), (5), (1), (1)
Host
Mouse
(120), (30), (8), (7), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(9), (7), (6), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Fluorescence Microscopy (FM), Flow Cytometry (FACS)
(120), (64), (40), (32), (31), (21), (20), (12), (7), (5), (1), (1)
Pubmed 1 reference available
Quantity 100 μL
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Catalog No. ABIN969396
363.00 $
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Immunogen Purified recombinant fragment of human SIRT1 expressed in E. coli.
Clone 1F3
Isotype IgG1
Purification Ascites
Alternative Name SIRT1
Background Synonyms:
SIR2L1, SIRT1
The Sir2 protein in yeast is known to function in transcriptional silencing processes through the deacetylation of histones H3 and H4. The more recently described human homologue of Sir2, known as SIRT1, has been found to associate with the tumor suppressor protein p53. SIRT1 binds and deacetylates p53 with specificity for its C-terminal Lys382 residue in response to the upregulation of promyelocytic leukemia protein (PML) nuclear bodies or oncogenic Ras. The deacetylation of p53 SIRT1 has been shown to negatively regulate p53-mediated transcription, preventing cellular senescence and apoptosis induced by DNA damage and stress. SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fηl and adult tissues, with high expression in heart, brain and skelηl muscle and low expression in lung and placenta. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.
Molecular Weight 120 kDa
Gene ID 23411
Research Area Chromatin and Nuclear Signaling, Apoptosis/Necrosis, Proteases
Application Notes Recommended Dilution:
ELISA: 1/10000,
WB: 1/500 - 1/2000,
IHC: 1/200 - 1/1000,
ICC: 1/200 - 1/1000,
FCM: 1/200 - 1/400

Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
anti-Sirtuin 1 (SIRT1) antibody Figure 4: Flow cytometric analysis of K562 cells using SIRT1 mouse mAb (green) and negative control (purple) .
anti-Sirtuin 1 (SIRT1) antibody (2) Figure 3: Immunofluorescence analysis of NTERA-2 cells using SIRT1 mouse mAb (green) . Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
anti-Sirtuin 1 (SIRT1) antibody (3) Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB staining.
anti-Sirtuin 1 (SIRT1) antibody (4) Figure 1: Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), Hela (3), HEK293 (4) and A549 (5) cell lysate.
Product cited in: Xie, Park, Kim et al.: "Transcriptional corepressor SMILE recruits SIRT1 to inhibit nuclear receptor estrogen receptor-related receptor gamma transactivation." in: The Journal of biological chemistry, Vol. 284, Issue 42, pp. 28762-74, 2009 (PubMed).

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