Sirtuin 1 (SIRT1) antibody

Antigen: Sirtuin 1 (SIRT1)

Synonyms: SIR2L1, SIRT1, SIR2, sirtuin 1, SRT1

Clonality: Monoclonal (1F3)

Host: Mouse

Reactivity: Human, Monkey

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB)

Catalog no.: ABIN969396

Additional Information

Alternative name: SIRT1

Gene ID: 23411

Immunogen: Purified recombinant fragment of human SIRT1 expressed in E. Coli.

Format: Liquid

Isotype: IgG1

Clone: 1F3

Description: The Sir2 protein in yeast is known to function in transcriptional silencing processes through the deacetylation of histones H3 and H4. The more recently described human homologue of Sir2, known as SIRT1, has been found to associate with the tumor suppressor protein p53.SIRT1 binds and deacetylates p53 with specificity for its C-terminal Lys382 residue in response to the upregulation of promyelocytic leukemia protein (PML) nuclear bodies or oncogenic Ras. The deacetylation of p53 SIRT1 has been shown to negatively regulate p53-mediated transcription, preventing cellular senescence and apoptosis induced by DNA damage and stress.SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues, with high expression in heart, brain and skeletal muscle and low expression in lung and placenta. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.
Synonyms: SIR2L1, SIRT1

Molecular Weight: 120kDa

Application Details

Application Notes: Western Blotting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Purification: Ascites

Buffer: Ascitic fluid containing 0.03% sodium azide.

Preservative: 0.03% sodium azide

Storage: Store at 4℃, for long term storage, store at -20℃.

Research Area: Chromatin and Nuclear Signaling, Apoptosis/Necrosis, Proteases

Restrictions: For Research Use only

Publications

Product: Xie, Park, Kim et al.: "Transcriptional corepressor SMILE recruits SIRT1 to inhibit nuclear receptor estrogen receptor-related receptor gamma transactivation." in: The Journal of biological chemistry, Vol. 284, Issue 42, pp. 28762-74, 2009 (PubMed).