SensoLyte® 520 Mouse Renin Assay Kit

Catalog No. ABIN1882547

Quick Overview for SensoLyte® 520 Mouse Renin Assay Kit (ABIN1882547)

Target: Renin (REN)

Reactivity: Mouse

Detection Method: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Product Details

Brand: SensoLyte®

Characteristics: The SensoLyte® 520 Mouse Renin Assay Kit provides a convenient assay for high throughput screening of renin inhibitors and for continuous assay of mouse renin activity using a 5-FAM/QXL™520 FRET peptide. In the FRET peptide the fluorescence of 5-FAM is quenched by QXL™520. Upon cleavage into two separate fragments by renin, the fluorescence of 5-FAM is recovered, and can be monitored at excitation/emission = 490/520 nm.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Storage: -80 °C

Storage Comment: Store all kit components at -20 °C, except for Component C. Store Component C at -80 °C. Protect Components A, B, F, and G from light and moisture. Component D can be stored at room temperature for convenience.

References

Takada, Kinugawa, Hirabayashi, Suga, Yokota, Takahashi, Fukushima, Homma, Ono, Sobirin, Masaki, Mizushima, Kadoguchi, Okita, Tsutsui: "Angiotensin II receptor blocker improves the lowered exercise capacity and impaired mitochondrial function of the skeletal muscle in type 2 diabetic mice." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 114, Issue 7, pp. 844-57, (2013) (PubMed).

Kakoki, Pochynyuk, Hathaway, Tomita, Hagaman, Kim, Zaika, Mamenko, Kayashima, Matsuki, Hiller, Li, Xu, Grant, Bertorello, Smithies: "Primary aldosteronism and impaired natriuresis in mice underexpressing TGF?1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 14, pp. 5600-5, (2013) (PubMed).

Kong, Zhu, Shi, Liu, Chen, Bhan, Zhao, Thadhani, Li: "VDR attenuates acute lung injury by blocking Ang-2-Tie-2 pathway and renin-angiotensin system." in: Molecular endocrinology (Baltimore, Md.), Vol. 27, Issue 12, pp. 2116-25, (2013) (PubMed).

Prysyazhna, Rudyk, Eaton: "Single atom substitution in mouse protein kinase G eliminates oxidant sensing to cause hypertension." in: Nature medicine, Vol. 18, Issue 2, pp. 286-90, (2012) (PubMed).

Wang, Deb, Zhang, Sun, Liu, Yoon, Kong, Chen, Chang, Li: "Vitamin D receptor signaling in podocytes protects against diabetic nephropathy." in: Journal of the American Society of Nephrology : JASN, Vol. 23, Issue 12, pp. 1977-86, (2012) (PubMed).

Target Details

Target: Renin (REN)

Alternative Name: Renin

Background: Renin, a highly specific aspartyl protease, cleaves angiotensinogen, to yield angiotensin I. Angiotensin I is further converted into angiotensin II by ACE (Angiotensin Converting Enzyme). Angiotensin II constricts blood vessels leading to increased blood pressure. Discovery of new renin inhibitors for treatment of hypertension is a promising area of research.