TNF alpha antibody

Catalog No. ABIN2192066

The Mouse Monoclonal anti-TNF alpha antibody has been validated for IA and InhA. It is suitable to detect TNF alpha in samples from Human. There are 2+ publications available.

Quick Overview for TNF alpha antibody (ABIN2192066)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Immunoassay (IA), Inhibition Assay (InhA)

Clone: 5N

Product Details anti-TNF alpha Antibody

Sterility: 0.2 μm filtered

Application Details

Application Notes: For neutralization of biological activity in vitro dilutions have to be made according to the amounts of -10 TNF-alpha to be inactivated. The affinity of the monoclonal antibody 5N is 5.7 x 10 M. The homologous ELISA is useful for the detection of oligomeric human TNF-alpha. It does not recognize monomeric TNF-alpha.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References for anti-TNF alpha antibody (ABIN2192066)

Song, Fox, Gallo, Rosenberg, Jordan, Shealy, Wagner: "Effects of 2 different anti-tumor necrosis factor-alpha agents in a primate model of subcutaneous abscess formation." in: The Journal of infectious diseases, Vol. 185, Issue 2, pp. 204-13, (2002) (PubMed).

Petyovka, Lyach, Voitenok: "Homologous ELISA for detection of oligomeric human TNF: properties of the assay." in: Journal of immunological methods, Vol. 186, Issue 2, pp. 161-70, (1995) (PubMed).

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: Tumor Necrosis Factor alpha

Background: The monoclonal antibody 5N reacts with Tumor Necrosis Factor alpha (TNF-alpha). Tumor necrosis factor-alpha (TNF-alpha), a homotrimeric 17 KD protein, is a potent mediator of inflammatory and metabolic functions. TNF-alpha was originally detected as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Further TNF-alpha has been implied as central mediator in shock induced by gram negative micro-organisms. The cytokine TNF-alpha is found to be a central mediator in many inflammatory and immunological processes: it can be induced by various products of micro-organisms and by various cytokines but it also induces on its turn the production of many cytokines. Signal transduction occurs via two types of TNF-receptors, the TNF-receptors I and II. The receptors differ strongly in their intra-cellular signaling pathways. The TNF-alpha trimer interacts with either of the two types of TNF-R leading to receptor cross-linking. The monoclonal antibody 5N cross-reacts and cross-neutralizes all monkey species TNF-alpha, except pig-tail macaque. With affinity about two orders lower it recognizes bovine and canine TNF-alpha. Aliases TNF, TNF-SF2, DIF, Cachectin

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome