anti-Bestrophin 1 (BEST1) Antibodies

BEST1 encodes a member of the bestrophin gene family.

list all antibodies Gene Name GeneID UniProt
Anti-Human BEST1 BEST1 7439 O76090
Anti-Mouse BEST1 BEST1 24115 O88870
Anti-Rat BEST1 BEST1 293735  
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Top anti-Bestrophin 1 Antibodies at

Showing 10 out of 47 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Dog Mouse Un-conjugated ICC, IHC (fro), IF, IHC (p), IP, WB Western blot detection of Bestrophin (68 kDa) from human RPE cell lysate using ABIN152509 (1:1000). 0.1 mL Log in to see 7 to 9 Days
Human Rabbit Un-conjugated WB WB Image Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 C: SK-N-SH 7.5% SDS PAGE antibody diluted at 1:1000 100 μL Log in to see 1 to 2 Days
Human Rabbit Un-conjugated IHC, WB Immunohistochemical analysis of Bestrophin-1 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Western blot analysis of Bestrophin-1 expression in PC3 (A), mouse brain (B), rat brain (C) whole cell lysates. 200 μL Log in to see 13 to 14 Days
Human Rabbit Un-conjugated WB Immunohistochemistry (IHC) analysis of paraffin-embedded Rat Brain Tissue using Bestrophin-1 Rabbit Polyclonal Antibody diluted at 1:200. Western Blot (WB) analysis of 1) PC3, 2)Mouse Brain Tissue, 3) Rat Brain Tissue with Bestrophin-1 Rabbit Polyclonal Antibody diluted at 1:2000. 100 μL Log in to see 16 Days
Human Rabbit Un-conjugated IHC, WB Western blot analysis of extracts of various cell lines, using BEST1 antibody. 100 μL Log in to see 16 Days
Human Mouse Un-conjugated WB Western Blot analysis of BEST1 expression in transfected 293T cell line by BEST1 MaxPab polyclonal antibody.<br><br>Lane 1: VMD2 transfected lysate(66.44 KDa).<br>Lane 2: Non-transfected lysate.<br> 50 μL Log in to see 11 to 12 Days
Human Rabbit Un-conjugated FACS, ICC, IHC, WB Western blot analysis of Bestrophin using anti-Bestrophin antibody .  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each  Lane was loaded with 50ug of sample under reducing conditions.   Lane 1: human A549 whole cell lysates,  Lane 2: human SGC-7901 whole cell lysates,  Lane 3: human U20S whole cell lysates.  After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bestrophin antigen affinity purified polyclonal antibody (Catalog # ) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bestrophin at approximately 68KD. The expected band size for Bestrophin is at 68KD. Flow Cytometry analysis of Hela cells using anti-BEST1 antibody . Overlay histogram showing Hela cells stained with  (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BEST1 Antibody (,1µg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. 100 μg Log in to see 4 to 6 Days
Human Mouse Un-conjugated ELISA, WB   100 μg Log in to see 7 to 9 Days
Human Rabbit Un-conjugated WB   50 μL Log in to see 7 to 9 Days
Human Mouse Un-conjugated ELISA, WB Western Blot detection against Immunogen (36.74 KDa) . 100 μg Log in to see 11 to 12 Days

Top referenced anti-Bestrophin 1 Antibodies

  1. Dog (Canine) Monoclonal Bestrophin 1 Primary Antibody for ICC, IHC (fro) - ABIN152509 : Klimanskaya, Hipp, Rezai, West, Atala, Lanza: Derivation and comparative assessment of retinal pigment epithelium from human embryonic stem cells using transcriptomics. in Cloning and stem cells 2005 (PubMed)
    Show all 26 Pubmed References

  2. Human Polyclonal Bestrophin 1 Primary Antibody for WB - ABIN521346 : Kuo, Abdullaev, Hyzinski-García, Mongin: Effects of alternative splicing on the function of bestrophin-1 calcium-activated chloride channels. in The Biochemical journal 2014 (PubMed)

More Antibodies against Bestrophin 1 Interaction Partners

Fruit Fly (Drosophila melanogaster) Bestrophin 1 (BEST1) interaction partners

  1. Bestrophin 1 (dBest1) as the Drosophila Cl(swell) channel

  2. bestrophin-1 chloride current is dually regulated by calcium and cell volume

Human Bestrophin 1 (BEST1) interaction partners

  1. These results revealed that the disease-causing mechanism of BEST1 mutations is centered on the indispensable role of BESTROPHIN1 in mediating the long speculated Ca(2 (show CA2 Antibodies)+)-dependent Cl(-) current in retinal pigment epithelium.

  2. We describe a highly recognizable and reproducible retinal phenotype associated with a specific BEST1 mutation-p.Ala243Val in vitelliform macular dystrophy.

  3. We have shown that the two novel combinations of compound heterozygous mutations p.R141H/p.M325T and p.R141H/p.I201T in the BEST1 gene can also lead to the autosomal recessive bestrophinopathy phenotype.

  4. The findings in this family emphasize the previously noted variability of clinical manifestations in BEST1-associated autosomal dominant vitreoretinochoroidopathy (ADVIRC) and the relevance of FAF (show FANCF Antibodies) and NIA imaging. Cystoid macular edema and vascular leakage can be successfully treated using dorzolamide.

  5. For patients with Best vitelliform macular dystrophy, single heterozygous BEST1 mutations were identified in 13 patients and compound heterozygous mutations were found in 3 patients. For patients with autosomal recessive bestrophinopathy, biallelic mutations were found in 13 probands and single mutant alleles in six patients. Overall, 36 disease-causing variants (20 novel mutations) of the BEST1 gene were identified.

  6. Mutation in BEST1 has variable penetrance and expressivity, and can be uniocular.

  7. We identified 7 BEST1 variants, 2 of which were new in 9 cases of Japanese patients with autosomal recessive bestrophinopathy.

  8. Of the 225 genetic tests performed, 150 were for recessive IRD (show SCRIB Antibodies), and 75 were for dominant IRD (show SCRIB Antibodies). A positive molecular diagnosis was made in 70 (59%) of probands with recessive IRD (show SCRIB Antibodies) and 19 (26%) probands with dominant IRD (show SCRIB Antibodies). Thirty-two novel variants were identified; among these, 17 sequence changes in four genes were predicted to be possibly or probably damaging including: ABCA4 (show ABCA4 Antibodies) (14), BEST1 (2), PRPH2 (show PRPH2 Antibodies) (1), and TIMP3 (show TIMP3 Antibodies)

  9. We describe the atypical phenotype and high intrafamilial variability associated with a new mutation in the BEST1 gene in an Italian family affected with Best vitelliform macular dystrophy.

  10. A clinical picture similar to autosomal recessive bestrophinopathy can also be caused by a single heterozygous mutation in the BEST1 gene, such as the c.614T>C (p.I205T) variant in this family.

Mouse (Murine) Bestrophin 1 (BEST1) interaction partners

  1. Data suggest that the ion channels CaV1.3 (show CACNA1D Antibodies), bestrophin-1 and maxiK (show KCNMA1 Antibodies) were identified as players in the regulation of photoreceptor outer segments (POS) phagocytosis by the retinal pigment epithelium (RPE (show RPE Antibodies)).

  2. Best1-mediated astrocytic glutamate (show GRIN1 Antibodies) activates the synaptic N-methyl-D-aspartate receptor (show GRIN1 Antibodies) (NMDAR (show GRIN1 Antibodies)) and modulates NMDAR (show GRIN1 Antibodies)-dependent synaptic plasticity.

  3. Bestrophin-1 functions as an intracellular Cl channel which helps to accumulate and to release Ca(2 (show CA2 Antibodies)+) from stores by conducting the counterion for Ca(2 (show CA2 Antibodies)+).

  4. Best1, located at the microdomains near the synaptic junctions, has a significantly high permeability to glutamate (show GRIN1 Antibodies) in vivo.

  5. Results show that different mutations in Best1 cause differential effects on its localization and that this effect varies with the presence or absence of wild-type (WT) Best1.

  6. Astrocytic glutamate (show GRIN1 Antibodies) via Best1 channel targets and activates synaptic NMDA receptors.

  7. Upon activation of protease activated receptor 1 (PAR1 (show F2R Antibodies)), an increase in intracellular Ca2 (show CA2 Antibodies)+ concentration leads to an opening of Best1 channels and subsequent release of glutamate (show GRIN1 Antibodies) in cultured astrocytes.

  8. Ultrastructural analyses demonstrate that TREK-1 (show KCNK2 Antibodies) is preferentially localized at cell body and processes, whereas Best1 is mostly found in microdomains of astrocytes near synapses.

  9. study reports that tonic inhibition in the cerebellum is due to GABA being released from glial cells by permeation through the Bestrophin 1 anion channel

  10. SOX9 (show SOX9 Antibodies), through interaction with microphthalmia-associated transcription factor (MITF (show MITF Antibodies)) and OTX2 (show OTX2 Antibodies), regulates BEST1 expression in the retinal pigment epithelium.

Pig (Porcine) Bestrophin 1 (BEST1) interaction partners

  1. Bestrophin-1 knock-down increases phagocytosis in primary porcine RPE (show RPE Antibodies) cells.

  2. Retinal pigment epithelium bestrophin-1 possibly conducts Cl(-) as counter ion for Ca(2 (show CA2 Antibodies)+) uptake into cytosolic Ca(2 (show CA2 Antibodies)+) stores.

  3. examined the quaternary structure of native best-1 and found that it migrates as a single species with a Stokes radius of 7.3 nm, sedimentation coefficient (S20 (show RPS20 Antibodies),w) of 4.9, and partial specific volume (nu) of 0.80 ml/g

Bestrophin 1 (BEST1) Antigen Profile

Protein Summary

This gene encodes a member of the bestrophin gene family. This small gene family is characterized by proteins with a highly conserved N-terminus with four to six transmembrane domains. Bestrophins may form chloride ion channels or may regulate voltage-gated L-type calcium-ion channels. Bestrophins are generally believed to form calcium-activated chloride-ion channels in epithelial cells but they have also been shown to be highly permeable to bicarbonate ion transport in retinal tissue. Mutations in this gene are responsible for juvenile-onset vitelliform macular dystrophy (VMD2), also known as Best macular dystrophy, in addition to adult-onset vitelliform macular dystrophy (AVMD) and other retinopathies. Alternative splicing results in multiple variants encoding distinct isoforms.

Gene names and symbols associated with anti-Bestrophin 1 (BEST1) Antibodies

  • Bestrophin 1 (Best1) antibody
  • bestrophin 1 (BEST1) antibody
  • bestrophin 1 (best1) antibody
  • Bestrophin 1 (Bm1_25500) antibody
  • bestrophin 1 (Best1) antibody
  • anon-WO0118547.380 antibody
  • ARB antibody
  • BEST antibody
  • best-1 antibody
  • BEST1 antibody
  • Bmd antibody
  • CG6264 antibody
  • Dbest antibody
  • dbest1 antibody
  • dmBest1 antibody
  • Dmel\\CG6264 antibody
  • mBest1 antibody
  • RP50 antibody
  • TU15B antibody
  • Vmd2 antibody

Protein level used designations for anti-Bestrophin 1 (BEST1) Antibodies

Best1-PA , Best1-PB , Best1-PC , Best1-PD , CG6264-PA , CG6264-PB , CG6264-PC , CG6264-PD , bestrophin 1 , bestrophin-1 , Bestrophin 1 , bestrophin-1-like , Best disease , Best1V1Delta2 , vitelliform macular dystrophy protein 2 , best macular dystrophy , vitelliform macular dystrophy 2 homolog , vitelliform macular dystrophy protein 2 homolog , vitelliform macular dystrophy (Best disease, bestrophin) , Bestrophin-1

53431 Drosophila melanogaster
466630 Pan troglodytes
100302038 Xenopus (Silurana) tropicalis
423124 Gallus gallus
483791 Canis lupus familiaris
508924 Bos taurus
6100000 Brugia malayi
100434568 Pongo abelii
7439 Homo sapiens
24115 Mus musculus
293735 Rattus norvegicus
397169 Sus scrofa
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