anti-Carbonic Anhydrase II (CA2) Antibodies

CA2 is one of several (at least 7) isozymes of carbonic anhydrase. Additionally we are shipping Carbonic Anhydrase II Kits (75) and Carbonic Anhydrase II Proteins (60) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
CA2 12349 P00920
CA2 54231 P27139
CA2 760 P00918
How to order from antibodies-online
  • +1 877 302 8632
  • +1 888 205 9894 (toll-free)
  • Order online

Top anti-Carbonic Anhydrase II Antibodies at

Showing 10 out of 266 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Delivery Price Details
Cow Rabbit Un-conjugated WB 100 μL 2 to 3 Days
Cow Rabbit Un-conjugated WB 100 μL 2 to 3 Days
Human Rabbit Un-conjugated IHC (p), WB Western blot analysis of CA2 using anti- CA2 antibody .  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each  Lane was loaded with 50ug of sample under reducing conditions.   Lane 1: rat ovary tissue lysates,   Lane 2: mouse liver tissue lysates,   Lane 3: MCF-7 whole cell lysates.  After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- CA2 antigen affinity purified polyclonal antibody (Catalog # ) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CA2 at approximately 29KD. The expected band size for CA2 is at 29KD。 IHC analysis of CA2 using anti- CA2 antibody . CA2 was detected in paraffin-embedded section of human gastric cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti- CA2 Antibody  overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. 100 μg 4 to 6 Days
Human Rabbit Un-conjugated IHC, WB Immunohistochemical analysis of Carbonic Anhydrase 2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Western blot analysis of Carbonic Anhydrase 2 expression in Caco2 (A), K562 (B), Caki1 (C) whole cell lysates. 200 μL 13 to 14 Days
Human Rabbit Un-conjugated IF, WB Western blot analysis of CA2 Antibody (N-term) (ABIN652314) in mouse kidney tissue lysates (35 µg/lane). CA2 (arrow) was detected using the purified polyclonal antibody. Western blot analysis of CA2 (arrow) using rabbit polyclonal CA2 Antibody (N-term) (ABIN652314). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CA2 gene. 400 μL 10 to 11 Days
Human Rabbit Un-conjugated ELISA, IHC, WB Western blot analysis of Mouse kidney lysate, using CA2 Antibody. The lane on the left is treated with the antigen-specific peptide. Western blot analysis of CA2 expression in various lysates 100 μL 11 to 12 Days
Human Rabbit Un-conjugated IHC (p), WB IHC-P Image Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using Carbonic anhydrase 2, antibody at 1:100 dilution. WB Image Sample (30 ug of whole cell lysate) A: 293T B: A431 , 12% SDS PAGE antibody diluted at 1:1000 100 μL 3 to 4 Days
Rat Rabbit Un-conjugated ICC, IHC, WB Western Blot; Sample: Mouse Heart lysate; Primary Ab: 2µg/ml Rabbit Anti-Rat CA2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) Western Blot; Sample: Rat Serum; Primary Ab: 2µg/ml Rabbit Anti-Rat CA2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) 100 μg 15 to 18 Days
Mouse Rabbit Un-conjugated IHC (p) Immunochemical staining of mouse CA2 in mouse brain with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections). Immunochemical staining of mouse CA2 in mouse intestine with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections). 100 μL 14 to 16 Days
Human Rabbit Un-conjugated ELISA, IHC, WB Western Blot (WB) analysis of Mouse Kidney Mouse Brain 293T HeLa lysis using CA II antibody. 100 μL Available

Top referenced anti-Carbonic Anhydrase II Antibodies

  1. Human Polyclonal CA2 Primary Antibody for ID, IF - ABIN2477889 : Uggeri, Proctor, Johns: Halothane, enflurane, and isoflurane attenuate both receptor- and non-receptor-mediated EDRF production in rat thoracic aorta. in Anesthesiology 1992 (PubMed)
    Show all 4 Pubmed References

  2. Human Polyclonal CA2 Primary Antibody for WB - ABIN1882068 : Fisher, Kovalevsky, Domsic, Mustyakimov, Silverman, McKenna, Langan: Preliminary joint neutron and X-ray crystallographic study of human carbonic anhydrase II. in Acta crystallographica. Section F, Structural biology and crystallization communications 2009 (PubMed)
    Show all 2 Pubmed References

More Antibodies against Carbonic Anhydrase II Interaction Partners

Xenopus laevis Carbonic Anhydrase II (CA2) interaction partners

  1. a bicarbonate transport metabolon proposed for the interaction between CAII and NBCe1 does not work at least in Xenopus oocytes

Arabidopsis thaliana Carbonic Anhydrase II (CA2) interaction partners

  1. Cytoplasmic CA2 plays an important role in amino acid biosynthesis and plant growth at low CO2.

  2. CA2 is an integral membrane protein localized on the matrix-exposed side of the membrane arm of complex I.

Mouse (Murine) Carbonic Anhydrase II (CA2) interaction partners

  1. It was concluded that both MCT1 and CAII are involved in the homeostatic control of pH in skeletal muscle, both at rest and at the onset of exercise. The improved muscle function and resistance to fatigue in MCT1(+/-) mice remain unexplained.

  2. two CA isoforms, CAII and CAIV, are distributed along the epididymal epithelium and appear with the onset of puberty

  3. The Na(+)-Cl(-) cotransporter NCC /Carbonic anhydrase II double Knockout mice displayed significant downregulation of pendrin, along with polyuria and salt wasting.

  4. CA2 expression is strongly regulated by intracellular pH and by cAMP, suggesting a role for soluble adenylyl cyclase

  5. sclerostin upregulated osteocyte expression of carbonic anhydrase 2.

  6. During inner ear development, transcripts of four cytosolic isozymes (Car1, Car2, Car3, and Car13), two membrane-bound isozymes (Car12 and Car14), and two CA-related proteins (Car8 and Car11) were expressed at higher levels than other isozymes.

  7. Car2, Car3, Car7, and Car15 mRNAs were barely within the detection limits in the mouse harderian gland.

  8. Suggest that during amelogenesis CAII controls the differentiation of ameloblasts. Regulation of intracellular pH, followed by activation of the JNK signaling pathway, may be responsible for the effects of CAII on ameloblasts.

  9. Both kNBC1-Asp(986) and kNBC1-Asp(988), but not kNBC1-Asp(989), are required for the phosphorylation-induced shift in stoichiometry. A homologous motif (D887ADD) in the carboxy-terminus of the anion exchanger AE1 binds to carbonic anhydrase II.

  10. carbonic anhydrases have a functional role specifically in endometrial gland development during postnatal mouse uterine morphogenesis.

  11. Results show that the stimulatory effect of the duodenal secretagogue prostaglandin E2 completely depends on carbonic anhydrase II.

  12. Altered expression of Car2 and Ncam1 is a downstream event in placental hyperplasia.

  13. These results suggest that the loss of function of cytosolic CA II in the stomach of Car2-/- mice leads to up-regulation of an extracellular CA, namely CA IX, which is expressed on the cell surface of the gastric epithelium.

  14. Expression in pancreas is restricted to cells within ductal structures and glucagon-positive cells with no colocalization with any insulin-positive cells at any time.

  15. In conclusion, skeletal muscle from mice deficient in CA II exhibits altered handling of acid-base challenges and shows normal contractile behavior at normal intracellular pH.

  16. identification of serum CA-II as an acute-phase protein; first report that CA-II circulates in the serum following inflammation

  17. Immunoblot analyses showed that the levels of carbonic anhydrase II are increased in the brain of Ts65Dn mice, a mouse model for Down syndrome.

  18. The metaphyseal widening and changes in the trabecular bone are consistent with osteopetrosis, making the CAII-deficient mouse a valuable model of human disease.

  19. CAII deficiency results in a significant decrease in the gene and protein expression of bicarbonate transport proteins from Slc26 gene family - Slc26a4 (pendrin) and Slc26a7.

  20. Carbonic anhydrase II is important for duodenocyte acidification by low luminal pH and for eliciting the acid-mediated HCO(3)(-) secretory response.

Human Carbonic Anhydrase II (CA2) interaction partners

  1. Study shows that proton-driven lactate flux is enhanced by the intracellular carbonic anhydrase CAII, which is colocalized with the monocarboxylate transporter MCT1 in MCF-7 breast cancer cells; the results suggest that CAII features a moiety that exclusively mediates proton exchange with the MCT to facilitate transport activity.

  2. the 1.6 A resolution structure of an imidazole-based CA activator in complex with the ubiquitously-expressed human CA II, is reported.

  3. Data report the crystal structure of HCAII. The structural changes seen are consistent with each of the six mutations involved acting largely independently and variously resulting in increased H-bonding, improved packing, and reduced side chain entropy loss on folding to yield the increased stability. These results suggest that for 4 mutations, improvements in backbone conformational energetics is also a contributor.

  4. CAII, CAIX, and CAXII all have characteristic expression patterns in esophageal adenocarcinoma (EAC), its precursors and normal squamous epithelium. In EAC, CAII downregulation is associated with metastatic disease. CAIX expression is lost towards the more malignant lesions, but in lymph node positive disease, expression seems to be higher. CAXII is only expressed in normal esophageal epithelium.

  5. present results suggest that CA2 serves as a suppressor of hepatocellular carcinoma metastasis and epithelial-mesenchymal transition and is correlated with favorable overall survival (OS) in hepatocellular carcinoma patients.

  6. The LC/MS analysis of platelet proteome between groups revealed that out of all identified proteins, the only discriminatory protein, affecting aspirin responsiveness, is platelet carbonic anhydrase II (CA II).

  7. Anti-CA I and II antibody levels were investigated using ELISA in serum samples from 30 patients with Acute Myeloid Leukemia (AML) and 30 healthy peers. Anti-CA I and II antibody titers in the AML group were significantly higher compared with the control group (p=0.0001 and 0.018, respectively).

  8. Study presented a detailed analysis of how protein dynamics (specifically the motions of nonreactive residues in the active site and coupled solvent dynamics) influence the rate limiting proton transfer/transport event in HCA II.

  9. These six-membered carbocycles showed nice inhibitory action against AChE and human carbonic anhydrase (hCA) II and I isoforms. The hCA I, II, and AChE were efficiently inhibited by these molecules, with Ki values in the range of 6.70-35.85 nM for hCA I, 18.77-60.84 nM for hCA II, and 0.74-4.60 for AChE, respectively

  10. These results showed that flavonoids especially malvin and oenin effectively inhibited hCA I and II isoenzymes.

  11. Discovery of potent carbonic anhydrase, acetylcholinesterase, and butyrylcholinesterase enzymes inhibitors: The new amides and thiazolidine-4-ones synthesized on an acetophenone base.

  12. This study provides the first evidence that extra-platelet nitrite and erythrocytic CAII may modulate platelet function in a cGMP-dependent manner.

  13. Here are presented solved the first crystal structures of carbonic anhydrase 1and carbonic anhydrase 2 in complex with polmacoxib, at 2.0 A and 1.8 A, respectively.

  14. CA II and CA XII, but not CA VII or CA IX, could be useful in predicting survival in colorectal carcinomas

  15. compute the hydration free energy of Zn(2+), the characteristics of hCAII-Zn(2+) complexation, and the absolute free energy of binding acetazolamide to the hCAII-Zn(2+) co

  16. This article presents different classes of structures of the mutant His-107-Tyr of human CA-II by harvesting molecular dynamics trajectories at low and high temperatures. Identified are putative unfolding intermediates of the mutant His-107-tyr of human carbonic anhydrase II in a multidimensional property space.

  17. The levels of expression of carbonic anhydrase 2, catalase, and PRDX2 in the nipple discharge were significantly increased in breast ductal carcinoma patients as compared to controls.

  18. This study suggests that the expression of CA II acts as independent prognostic biomarker for survival in pseudomyxoma peritonei

  19. Changes in membrane composition may affect the erythrocyte membranes' capacity to increase in vitro hCA II activity

  20. identified 2 novel mutations in 2 Chinese families with CAII deficiency syndrome; a nonsense mutation in exon 4 in both families; a splice mutation at the splice donor site of intron 3 in 1 family; the splice-site mutation causes exon 3 skipping in patient's mRNA resulting in an in-frame deletion and a novel premature stop codon

Cow (Bovine) Carbonic Anhydrase II (CA2) interaction partners

  1. This study provides the first evidence that extra-platelet nitrite and erythrocytic CAII may modulate platelet function in a cGMP-dependent manner.

  2. binding of dorzolamide to bovine and human carbonic anhydrase II

  3. Increasing the number and distribution of surface acidic groups of CAII is sufficient to function in high-salt environments.

  4. Folding analysis of the zinc protein, carbonic anhydrase 2 was performed using electrospray ionization ion mobility spectrometry coupled with collision-induced dissociation.

  5. Intense positive staining for CA-II was found in luminal duct cells and for the first time also inside the duct lumen, clearly demonstrating the expression and secretion of salivary CA-II in bovine parotid glands.

  6. structure refined to 1.95 A resolution; has a C-terminal knot structure; contains one zinc ion in the active site coordinated to 3 his and 1 water molecule; structure reveals a probable alternative proton-wire pathway that differs from that of HCA II.

  7. novel series of mono- and bis- benzene sulfonamides with potent binding affinity for bovine carbonic anhydrase II

  8. catalytic activity of CAII affects the SNAT3 conductance, but presumably not by increasing the local H+ buffer capacity

  9. kinetics of refolding of carbonic anhydrase II following transfer from a buffer containing 5 m guanidinium chloride to a buffer containing 0.5 m guanidinium chloride

  10. The urea-induced unfolding of bovine carbonic anhydrase II, BCA II, is characterized by a combination of high-resolution NMR, circular dichroism spectroscopy and small angle X-ray scattering.

  11. Carbonic anhydrase II (BCA II) when incubated in mildly denaturing conditions showed that the partially unfolded conformers assemble together and form ordered amyloid aggregates.

Carbonic Anhydrase II (CA2) Antigen Profile

Protein Summary

CA2 is one of several (at least 7) isozymes of carbonic anhydrase. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis.

Gene names and symbols associated with CA2

  • Carbonic anhydrase 2 (CAH2) antibody
  • carbonic anhydrase 2 L homeolog (ca2.L) antibody
  • carbonic anhydrase 2 (ca2) antibody
  • carbonic anhydrase 2 (CA2) antibody
  • carbonic anhydrase 2 (HCAG_04637) antibody
  • carbonic anhydrase 2 (LOC100135827) antibody
  • carbonic anhydrase 1 (LOC100635510) antibody
  • carbonic anhydrase 2 (Car2) antibody
  • carbonic anhydrase II (CA2) antibody
  • carbonic anhydrase 2 (Ca2) antibody
  • carbonic anhydrase 2 (LOC100154873) antibody
  • carbonic anhydrase 2 (LOC101113341) antibody
  • carbonic anhydrase, alpha type, periplasmic (CAH2) antibody
  • AI131712 antibody
  • BETA CA2 antibody
  • CA-2 antibody
  • CA-II antibody
  • Ca2 antibody
  • CA18 antibody
  • CAC antibody
  • CAII antibody
  • ca ii antibody
  • Car-2 antibody
  • Car2 antibody
  • CAR3 antibody
  • carbonic anhydrase 2 antibody
  • CARBONIC ANHYDRASE 18 antibody
  • CG6906 antibody
  • CT21171 antibody
  • Dmel\\CG6906 antibody
  • LOC100135827 antibody
  • Ltw-5 antibody
  • Lvtw-5 antibody
  • T9L3.40 antibody
  • T9L3_40 antibody

Protein level used designations for CA2

CAH2-PA , CAH2-PB , CG6906-PA , CG6906-PB , carbonic anhydrase 2 , carbonic anhydrase II , carbonic anhydrase type 2 , erythrocyte carbonic anhydrase , CA II , CA-II , carbonate dehydratase II , carbonic anhydrase B , carbonic anhydrase C , carbonic dehydratase

39390 Drosophila melanogaster
379772 Xenopus laevis
548446 Xenopus (Silurana) tropicalis
831326 Arabidopsis thaliana
5446993 Ajellomyces capsulatus NAm1
100135827 Oncorhynchus mykiss
100635510 Amphimedon queenslandica
12349 Mus musculus
54231 Rattus norvegicus
760 Homo sapiens
280740 Bos taurus
100009156 Oryctolagus cuniculus
100719293 Cavia porcellus
100154873 Sus scrofa
396257 Gallus gallus
101113341 Ovis aries
477928 Canis lupus familiaris
574263 Macaca mulatta
5717780 Chlamydomonas reinhardtii
Selected quality suppliers for anti-Carbonic Anhydrase II (CA2) Antibodies
Did you look for something else?