anti-Carbonic Anhydrase II (CA2) Antibodies

CA2 is one of several (at least 7) isozymes of carbonic anhydrase. Additionally we are shipping Carbonic Anhydrase II Kits (72) and Carbonic Anhydrase II Proteins (61) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
CA2 12349 P00920
CA2 54231 P27139
CA2 760 P00918
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Top anti-Carbonic Anhydrase II Antibodies at antibodies-online.com

Showing 10 out of 242 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Cow Rabbit Un-conjugated WB 100 μL Log in to see 2 to 3 Days
$289.00
Details
Cow Rabbit Un-conjugated WB 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Rabbit Un-conjugated IHC (p), WB Western blot analysis of CA2 using anti- CA2 antibody . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each Lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat ovary tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- CA2 antigen affinity purified polyclonal antibody (Catalog # ) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CA2 at approximately 29KD. The expected band size for CA2 is at 29KD。 IHC analysis of CA2 using anti- CA2 antibody . CA2 was detected in paraffin-embedded section of human gastric cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti- CA2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. 100 μg Log in to see 4 to 6 Days
$280.00
Details
Human Rabbit Un-conjugated IHC, WB Immunohistochemical analysis of Carbonic Anhydrase 2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Western blot analysis of Carbonic Anhydrase 2 expression in Caco2 (A), K562 (B), Caki1 (C) whole cell lysates. 200 μL Log in to see 13 to 14 Days
$487.50
Details
Human Rabbit Un-conjugated IF, WB Western blot analysis of CA2 Antibody (N-term) (ABIN652314) in mouse kidney tissue lysates (35 µg/lane). CA2 (arrow) was detected using the purified polyclonal antibody. Western blot analysis of CA2 (arrow) using rabbit polyclonal CA2 Antibody (N-term) (ABIN652314). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CA2 gene. 400 μL Log in to see 10 to 11 Days
$335.50
Details
Human Rabbit Un-conjugated IHC (p), WB IHC-P Image Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using Carbonic anhydrase 2, antibody at 1:100 dilution. WB Image Sample (30 ug of whole cell lysate) A: 293T B: A431 , 12% SDS PAGE antibody diluted at 1:1000 100 μL Log in to see 1 to 2 Days
$358.60
Details
Rat Rabbit Un-conjugated ICC, IHC, WB Western Blot; Sample: Mouse Heart lysate; Primary Ab: 2µg/ml Rabbit Anti-Rat CA2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) Western Blot; Sample: Rat Serum; Primary Ab: 2µg/ml Rabbit Anti-Rat CA2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) 100 μg Log in to see 15 to 18 Days
$342.00
Details
Mouse Rabbit Un-conjugated IHC (p) Immunochemical staining of mouse CA2 in mouse brain with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections). Immunochemical staining of mouse CA2 in mouse intestine with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections). 100 μg Log in to see 20 to 21 Days
$273.90
Details
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (p), WB Western Blot: Carbonic Anhydrase II/CA2 Antibody [NBP2-52736] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells).Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells).Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla) Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase II/CA2 Antibody [NBP2-52736] - Staining of human cell line U-251MG shows positivity in cytoplasm. 0.1 mL Log in to see 7 to 9 Days
$491.63
Details
Human Rabbit HRP ELISA, IHC, WB Western Blot of Rabbit anti-Carbonic Anhydrase II Antibody Peroxidase Conjugated. Lane 1: Carbonic Anhydrase II. Load: 50 ng per lane. Primary antibody: Rabbit anti-Carbonic Anhydrase II Antibody Peroxidase Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: n/a. Block: ABIN925618 for 30 minutes at RT. Predicted/Observed size: 29 kDa, 29 kDa for Carbonic Anhydrase II. 100 μg Log in to see 7 to 9 Days
$389.00
Details

CA2 Antibodies by Reactivity, Application, Clonality and Conjugate

Attributes Applications Host Clonality Conjugate
Mouse (Murine) , ,

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Top referenced anti-Carbonic Anhydrase II Antibodies

  1. Human Polyclonal CA2 Primary Antibody for ID, IF - ABIN2477889 : Uggeri, Proctor, Johns: Halothane, enflurane, and isoflurane attenuate both receptor- and non-receptor-mediated EDRF production in rat thoracic aorta. in Anesthesiology 1992 (PubMed)
    Show all 4 Pubmed References

  2. Human Polyclonal CA2 Primary Antibody for WB - ABIN1882068 : Fisher, Kovalevsky, Domsic, Mustyakimov, Silverman, McKenna, Langan: Preliminary joint neutron and X-ray crystallographic study of human carbonic anhydrase II. in Acta crystallographica. Section F, Structural biology and crystallization communications 2009 (PubMed)
    Show all 2 Pubmed References

More Antibodies against Carbonic Anhydrase II Interaction Partners

Xenopus laevis Carbonic Anhydrase II (CA2) interaction partners

  1. a bicarbonate transport metabolon proposed for the interaction between CAII and NBCe1 (show SLC4A4 Antibodies) does not work at least in Xenopus oocytes

Arabidopsis thaliana Carbonic Anhydrase II (CA2) interaction partners

  1. Cytoplasmic CA2 plays an important role in amino acid biosynthesis and plant growth at low CO2.

  2. CA2 is an integral membrane protein localized on the matrix-exposed side of the membrane arm of complex I.

Mouse (Murine) Carbonic Anhydrase II (CA2) interaction partners

  1. It was concluded that both MCT1 (show MCTS1 Antibodies) and CAII are involved in the homeostatic control of pH in skeletal muscle, both at rest and at the onset of exercise. The improved muscle function and resistance to fatigue in MCT1 (show MCTS1 Antibodies)(+/-) mice remain unexplained.

  2. two CA isoforms, CAII and CAIV (show CA4 Antibodies), are distributed along the epididymal epithelium and appear with the onset of puberty

  3. The Na(+)-Cl(-) cotransporter NCC (show SLC12A3 Antibodies) /Carbonic anhydrase II double Knockout mice displayed significant downregulation of pendrin (show SLC26A4 Antibodies), along with polyuria and salt wasting.

  4. CA2 expression is strongly regulated by intracellular pH and by cAMP, suggesting a role for soluble adenylyl cyclase (show ADCY10 Antibodies)

  5. sclerostin (show SOST Antibodies) upregulated osteocyte expression of carbonic anhydrase 2.

  6. During inner ear development, transcripts of four cytosolic isozymes (Car1 (show CA1 Antibodies), Car2, Car3 (show CA3 Antibodies), and Car13 (show CA13 Antibodies)), two membrane-bound isozymes (Car12 and Car14 (show CA14 Antibodies)), and two CA-related proteins (Car8 (show CA8 Antibodies) and Car11 (show CA11 Antibodies)) were expressed at higher levels than other isozymes.

  7. Car2, Car3, Car7, and Car15 mRNAs were barely within the detection limits in the mouse harderian gland.

  8. Suggest that during amelogenesis CAII controls the differentiation of ameloblasts. Regulation of intracellular pH, followed by activation of the JNK (show MAPK8 Antibodies) signaling pathway, may be responsible for the effects of CAII on ameloblasts.

  9. Both kNBC1 (show SLC4A4 Antibodies)-Asp (show C3 Antibodies)(986) and kNBC1 (show SLC4A4 Antibodies)-Asp (show C3 Antibodies)(988), but not kNBC1 (show SLC4A4 Antibodies)-Asp (show C3 Antibodies)(989), are required for the phosphorylation-induced shift in stoichiometry. A homologous motif (D887ADD) in the carboxy-terminus of the anion exchanger AE1 (show SLC4A1 Antibodies) binds to carbonic anhydrase II.

  10. Results show that the stimulatory effect of the duodenal secretagogue prostaglandin E2 completely depends on carbonic anhydrase II.

Human Carbonic Anhydrase II (CA2) interaction partners

  1. The LC/MS analysis of platelet proteome between groups revealed that out of all identified proteins, the only discriminatory protein, affecting aspirin responsiveness, is platelet carbonic anhydrase II (CA II).

  2. Anti-CA I (show CA1 Antibodies) and II antibody levels were investigated using ELISA in serum samples from 30 patients with Acute Myeloid Leukemia (show BCL11A Antibodies) (AML (show RUNX1 Antibodies)) and 30 healthy peers. Anti-CA I (show CA1 Antibodies) and II antibody titers in the AML (show RUNX1 Antibodies) group were significantly higher compared with the control group (p=0.0001 and 0.018, respectively).

  3. These six-membered carbocycles showed nice inhibitory action against AChE and human carbonic anhydrase (hCA) II and I isoforms. The hCA I, II, and AChE were efficiently inhibited by these molecules, with Ki values in the range of 6.70-35.85 nM for hCA I, 18.77-60.84 nM for hCA II, and 0.74-4.60 for AChE, respectively

  4. Discovery of potent carbonic anhydrase, acetylcholinesterase (show AChE Antibodies), and butyrylcholinesterase (show BCHE Antibodies) enzymes inhibitors: The new amides and thiazolidine-4-ones synthesized on an acetophenone base.

  5. This study provides the first evidence that extra-platelet nitrite and erythrocytic CAII may modulate platelet function in a cGMP-dependent manner.

  6. Here are presented solved the first crystal structures of carbonic anhydrase 1and carbonic anhydrase 2 in complex with polmacoxib, at 2.0 A and 1.8 A, respectively.

  7. CA II and CA XII, but not CA VII (show CA7 Antibodies) or CA IX (show CA9 Antibodies), could be useful in predicting survival in colorectal carcinomas

  8. This article presents different classes of structures of the mutant His-107-Tyr (show TYR Antibodies) of human CA-II by harvesting molecular dynamics trajectories at low and high temperatures. Identified are putative unfolding intermediates of the mutant His-107-tyr (show TYR Antibodies) of human carbonic anhydrase II in a multidimensional property space.

  9. The levels of expression of carbonic anhydrase 2, catalase (show CAT Antibodies), and PRDX2 (show PRDX2 Antibodies) in the nipple discharge were significantly increased in breast ductal carcinoma patients as compared to controls.

  10. This study suggests that the expression of CA II acts as independent prognostic biomarker for survival in pseudomyxoma peritonei

Cow (Bovine) Carbonic Anhydrase II (CA2) interaction partners

  1. This study provides the first evidence that extra-platelet nitrite and erythrocytic CAII may modulate platelet function in a cGMP-dependent manner.

  2. binding of dorzolamide to bovine and human carbonic anhydrase II

  3. Increasing the number and distribution of surface acidic groups of CAII is sufficient to function in high-salt environments.

  4. Folding analysis of the zinc protein, carbonic anhydrase 2 was performed using electrospray ionization ion mobility spectrometry coupled with collision-induced dissociation.

  5. Intense positive staining for CA-II was found in luminal duct cells and for the first time also inside the duct lumen, clearly demonstrating the expression and secretion of salivary CA-II in bovine parotid glands.

  6. structure refined to 1.95 A resolution; has a C-terminal knot (show KCNK7 Antibodies) structure; contains one zinc ion in the active site coordinated to 3 his and 1 water molecule; structure reveals a probable alternative proton-wire pathway that differs from that of HCA II.

  7. novel series of mono- and bis (show BAG3 Antibodies)- benzene sulfonamides with potent binding affinity for bovine carbonic anhydrase II

  8. catalytic activity of CAII affects the SNAT3 (show SLC38A3 Antibodies) conductance, but presumably not by increasing the local H+ buffer capacity

  9. kinetics of refolding of carbonic anhydrase II following transfer from a buffer containing 5 m guanidinium chloride to a buffer containing 0.5 m guanidinium chloride

  10. The urea-induced unfolding of bovine carbonic anhydrase II, BCA (show BLNK Antibodies) II, is characterized by a combination of high-resolution NMR, circular dichroism spectroscopy and small angle X-ray scattering.

Carbonic Anhydrase II (CA2) Antigen Profile

Protein Summary

CA2 is one of several (at least 7) isozymes of carbonic anhydrase. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis.

Gene names and symbols associated with CA2

  • Carbonic anhydrase 2 (CAH2) antibody
  • carbonic anhydrase 2 L homeolog (ca2.L) antibody
  • carbonic anhydrase 2 (ca2) antibody
  • carbonic anhydrase 2 (CA2) antibody
  • carbonic anhydrase 2 (HCAG_04637) antibody
  • carbonic anhydrase 2 (LOC100135827) antibody
  • carbonic anhydrase 1 (LOC100635510) antibody
  • carbonic anhydrase 2 (Car2) antibody
  • carbonic anhydrase II (CA2) antibody
  • carbonic anhydrase 2 (Ca2) antibody
  • carbonic anhydrase 2 (LOC100154873) antibody
  • carbonic anhydrase 2 (LOC101113341) antibody
  • carbonic anhydrase, alpha type, periplasmic (CAH2) antibody
  • AI131712 antibody
  • BETA CA2 antibody
  • BETA CARBONIC ANHYDRASE 2 antibody
  • CA-2 antibody
  • CA-II antibody
  • Ca2 antibody
  • CA18 antibody
  • CAC antibody
  • ca ii antibody
  • CAII antibody
  • Car-2 antibody
  • Car2 antibody
  • CAR3 antibody
  • carbonic anhydrase 2 antibody
  • CARBONIC ANHYDRASE 18 antibody
  • CG6906 antibody
  • CT21171 antibody
  • Dmel\\CG6906 antibody
  • LOC100135827 antibody
  • Ltw-5 antibody
  • Lvtw-5 antibody
  • T9L3.40 antibody
  • T9L3_40 antibody

Protein level used designations for CA2

CAH2-PA , CAH2-PB , CG6906-PA , CG6906-PB , carbonic anhydrase 2 , carbonic anhydrase II , carbonic anhydrase type 2 , erythrocyte carbonic anhydrase , CA II , CA-II , carbonate dehydratase II , carbonic anhydrase B , carbonic anhydrase C , carbonic dehydratase

GENE ID SPECIES
39390 Drosophila melanogaster
379772 Xenopus laevis
548446 Xenopus (Silurana) tropicalis
831326 Arabidopsis thaliana
5446993 Ajellomyces capsulatus NAm1
100135827 Oncorhynchus mykiss
100635510 Amphimedon queenslandica
12349 Mus musculus
54231 Rattus norvegicus
760 Homo sapiens
280740 Bos taurus
100009156 Oryctolagus cuniculus
100719293 Cavia porcellus
100154873 Sus scrofa
396257 Gallus gallus
101113341 Ovis aries
477928 Canis lupus familiaris
574263 Macaca mulatta
5717780 Chlamydomonas reinhardtii
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