anti-Cation Channel, Sperm Associated 1 (CATSPER1) Antibodies

Horse (Equine) Cation Channel, Sperm Associated 1 (CATSPER1) interaction partners

1. The CatSper channel is present in equine sperm but that the relationship of hyperactivated motility to calcium influx is weak.

Human Cation Channel, Sperm Associated 1 (CATSPER1) interaction partners

1. explored whether steroid hormones to which human spermatozoa are exposed in the male and female genital tract influence CatSper activation via modulation of ABHD2

2. Electrophysiological studies showed a significant increase in Cd(2+) and manganese (Mn(2+)) currents through the CaV3.1 mutants as well as a reduction in the inhibitory effect of Cd(2+) on the Ca(2+) current.

3. Sperm with a near absence of CatSper current failed to respond to activation of CatSper by progesterone and there was fertilization failure at IVF.

4. The role of CATSPER1 in idiopathic asthenospermia pathogenesis: exonal SNP rs1893316 in CATSPER1 significantly correlated with idiopathic asthenospermia risk

5. Sox5 and Sox9 cause a significant increase in transactivation of the Catsper1 promoter.

6. Progesterone via CatSper activates the PI3K-AKT pathway required for motility and hyperactivation but not for acrosome reaction.

7. This work reports the cloning and characterization of the promoter regions in the human and murine Catsper1 genes. The immediate upstream region and the first exon in the human CATSPER1 gene negatively regulate transcriptional activity. The mouse Catsper1 promoter exhibited transcriptional activity in both orientations and displayed significant expression levels in mouse testis in vivo.

8. CatSper is indeed the principal Ca2+ channel of human spermatozoa, and that it is strongly potentiated by progesterone.

9. CatSper activation can elicit functionally different behaviors according to the sensitivity of the Ca(2+) store, which may be regulated by capacitation and NO from the cumulus.

10. It was shown that odorants directly activate CatSper without involving G protein-coupled receptors and cAMP. Membrane-permeable analogues of cyclic nucleotides activated CatSper directly via an extracellular site.

11. The decreased or abnormal expression of the CatSper1 protein may be a factor involved in the pathogenesis of idiopathic asthenozoospermia.

12. progesterone activates the sperm-specific, pH-sensitive CatSper Ca(2+) channel

13. human CatSper is synergistically activated by elevation of intracellular pH and extracellular progesterone

14. Studies indicate that CATSPER channel represents a novel human male fertility factor.

15. Potential role for CatSper in sperm motility and fertility in mouse and human. CatSper is therefore implicated as a potential target to explore the molecular mechanisms of male infertility.

16. CATSPER1 is meiotically and post-meiotically expressed in human testis tissue.

17. CatSper1 and CatSper2 can associate with and modulate the function of the Ca(v)3.3 channel, which might be important in the regulation of sperm function.

18. CatSper1 may be a potential target for immunocontraception, and the antibody may be a tool to study the function of ion channels in sperm.

19. sequence analysis of CATSPER1 in heritable forms of nonsyndromic male infertility revealed two separate insertion mutations (c.539-540insT and c.948-949insATGGC) that are predicted to lead to frameshifts and premature stop codons

Mouse (Murine) Cation Channel, Sperm Associated 1 (CATSPER1) interaction partners

1. The results showed that hypothyroidism could adversely affect sperm morphology, sperm chromatin condensation, and CatSper gene expression in mice and these abnormalities may be related to the excessive production of reactive oxygen species (ROS) in a hypothyroid state.

2. Data suggest that Catsper1, Catsper2, Catsper3, and Catsper4 form protein multimer with hypothetical activities of flippase and disulfide isomerase.

3. Both in vivo administration and in vitro application of bisphenol A impair mature sperm functions by a CatSper-relevant mechanism.

4. patch clamping of sperm revealed that CRISP1 has the ability to regulate CatSper, the principal sperm Ca(2+) channel involved in hyperactivation and essential for fertility

5. Catsper transports the Ca(2+) involved in the regulation of cAMP-dependent and tyrosine phosphorylation pathways required for sperm capacitation.

6. These results establish a critical role of LRRC52 in KSPER channels and demonstrate that loss of a non-pore-forming auxiliary subunit results in severe fertility impairment.

7. SLO3 K+ channels have a role in controlling calcium entry through CATSPER channels in sperm

8. Using mice lacking both SLO3 and CATSPER1 subunits, the results show conclusively that the voltage-activated outward current present in Slo3 (-/-) sperm is abolished when CATSPER is also deleted.

9. The mouse Catsper1 promoter exhibited transcriptional activity in both orientations and displayed significant expression levels in the mouse testis in vivo.

10. Immunization with B-cell epitopes in transmembrane domains and pore region of CatSper1 can cause significant antifertility effects in male mice.

11. Yijingfang can increase the sperm count and the expression of CatSper1.

12. KSper/Slo3 is the primary spermatozoan K(+) channel,and KSper may play a critical role in acquisition of normal morphology and sperm motility when faced with hyperosmotic challenges.

13. The initial phase of the increases in intracellular calcium concentration induced by BSA requires the CATSPER channel, but not the voltage-gated calcium channel.

14. CatSper1 functions as a voltage-gated Ca2+ channel that controls Ca2+ entry to mediate the hyperactivated motility needed late in the preparation of sperm for fertilization

15. Potential role for CatSper in sperm motility and fertility in mouse and human. CatSper is therefore implicated as a potential target to explore the molecular mechanisms of male infertility.

16. Catsper1's function in sperm physiology and role in sperm competition using rodent models

17. CatSper1 and CatSper2 null sperm show identical phenotypes in mice

18. results identify CatSper1 as a component of the key flagellar calcium channel, and suggest that intracellular alkalinization potentiates CatSper current to increase intraflagellar calcium and induce sperm hyperactivation

19. Direct protein interactions among CatSpers, the sperm specificity of these proteins, and loss of ICatSper in each of the four CatSper-/- mice indicate that CatSpers are highly specialized flagellar proteins

20. Data show that there is a significant downregulation of CatSpers 1 following spinal cord injury.