Ceramide Kinase (CERK) ELISA Kits

Mouse (Murine) Ceramide Kinase (CERK) interaction partners

1. the data presented in this work suggest that exogenous C1P may balance adipogenesis, and that targeting CerK may be a novel way for potential applications in the treatment of obesity or other inflammation-associated diseases

2. results suggest that intracellular ceramide kinase is required for the migration of bone marrow-derived mesenchymal stem cells, and the roles of the intrinsic ceramide kinase in the migration are associated with N-cadherin regulation

3. The depletion of CerK enhances the pathology of colitis and lethal responses in DSS-treated mice.

4. these studies demonstrate a novel requirement for the CERK-derived C1P in the proper healing response of wounds.

5. there are significant differences in eicosanoid levels in ex vivo CERK(-/-) cells compared with wild-type counterparts, but the effect of the genetic ablation of CERK on eicosanoid synthesis and the serum levels of C1P was not apparent in vivo

6. role of ceramide kinase in obesity and insulin resistance

7. Results indicate that C1P produced by CERK has a negative effect on the processing/secretion of TNFalpha via modulation of TACE activity.

8. role of CerK on the activation of mast cells

9. The mechanism of ceramide-mediated inhibition is likely to involve conversion of ceramide to ceramide 1-phosphate by CERK.

10. LPS inhibits the transcriptional activity of the Cerk proximal promoter;transcriptional repression of Cerk by LPS is not a primary consequence of LPS-induced cell cycle blockade.

11. Findings suggest that CerK is not necessary for survival at an individual level, but might be involved in higher brain function related to emotion.

12. CerK is essential to ceramide 1-phosphate formation via phosphorylation of Cer

13. Ceramide kinase is a key regulator of ceramide 1-phosphate, dihydroceramide and ceramide levels, with important implications for neutrophil homeostasis and innate immunity regulation.

Human Ceramide Kinase (CERK) interaction partners

1. an acid sphingomyelinase ceramide kinase pathway in the regulation of the chemokine CCL5

2. Our study expands the genotypic spectra of CERKL variants, providing insights into the relevant pathogenesis of RP/CRD

3. results suggest that intracellular ceramide kinase is required for the migration of bone marrow-derived mesenchymal stem cells, and the roles of the intrinsic ceramide kinase in the migration are associated with N-cadherin regulation

4. our findings identify a functional role for Cerk in breast cancer recurrence and suggest the clinical utility of agents targeted against this prosurvival pathway.

5. Data suggest that inhibition of ceramide kinase (CerK) [by specific gene silencing or pharmacological inhibition] drastically reduced cell proliferation in a neuroblastoma cell line.

6. TNF-alpha exposure of human SH-SY5Y neuroblastoma caused a profound increase in CERK activity.

7. Expression of hCERK enhanced ceramide-1-phosphate formation and release of arachidonic acid in Ca(2+) ionophore-stimulated cells.

8. We have identified a differential role for ceramide kinase in mast-cell activation

9. Molecular cloning and functional characterization

10. ceramide kinase and its product, C-1-P, have roles in arachidonic acid release and production of eicosanoids

11. CERK translocates during activation from the cytosol to a lipid raft fraction

12. Pleckstrin homology domain of CERK is not only indispensable for its activity but also act as a regulator of CERK activity.

13. sphingoid chain was also required for substrate recognition by CERK

14. Recombinant CERK was analyzed with regard to dependence on divalent cations, to substrate delivery, specificity, and stereoselectivity. CERK associated with the plasma membrane in CHO cells, which is mediated by the N-terminal putative pleckstrin domain.

15. CERK-pleckstrin homology domain plays a crucial role in plasma membrane targeting through its binding to phosphatidylinositol 4,5-bisphosphate and subsequent induction of ceramide-1-phosphate production in the vicinity of the membrane.

16. These results suggest that ceramide kinase determines the balance between pro-apoptotic ceramide and anti-apoptotic C1P to regulate cell fate, reminiscent of its function in plants.

17. Results demonstrate that CERK localizes to areas of eicosanoid synthesis and uses a ceramide "pool" transported in an active manner via CERT.

18. HL-60 cells are useful for studying CerK functions in leukocyte differentiation, and they also suggest that CerK might have an important role in such differentiation.

19. higher Ca2+ signal observed in hCERK transfected cells as well as the fact that CERK colocalized with EIgG during phagocytosis support hypothesis that Ca2+ signaling is an important factor for increasing phagocytosis and is regulated by CERK

20. identification of a cluster of cysteines, C(347)XXXC(351)XXC(354), essential for CerK function