anti-Chromodomain Helicase DNA Binding Protein 4 (CHD4) Antibodies

The product of CHD4 belongs to the SNF2/RAD54 helicase family. Additionally we are shipping CHD4 Kits (6) and CHD4 Proteins (4) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
CHD4 1108 Q14839
CHD4 117535  
CHD4 107932 Q6PDQ2
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Top anti-CHD4 Antibodies at antibodies-online.com

Showing 10 out of 80 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Cow Rabbit Un-conjugated WB WB Suggested Anti-CHD4 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:12500 Positive Control: OVCAR-3 cell lysate There is BioGPS gene expression data showing that CHD4 is expressed in OVCAR3 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Rabbit Un-conjugated ICC, IF, IHC, WB Mi-2 beta antibody tested by immunofluorescence. Top left: HeLa cells stained with Mi-2 beta antibody (1:1,000). Top right: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Same cells stained with DAPI. Bottom right: Merge of all 3 images. Mi-2 beta antibody tested by Western blot. Whole-cell extract of mouse splenocytes stained with Mi-2 beta antibody (1:1,1000). 200 μL Log in to see 1 to 2 Days
$495.83
Details
Human Rabbit Un-conjugated IF, IHC, ELISA Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using CHD4 Antibody. The picture on the right is treated with the synthesized peptide. Immunofluorescence analysis of HepG2 cells, using CHD4 Antibody. The picture on the right is treated with the synthesized peptide. 100 μg Log in to see 2 to 3 Days
$302.50
Details
Human Mouse Un-conjugated WB Western Blot analysis of CHD4 expression in transfected 293T cell line by CHD4 monoclonal antibody (M01), clone 4H4. Lane 1: CHD4 transfected lysate(220 KDa). Lane 2: Non-transfected lysate. CHD4 monoclonal antibody (M01), clone 4H4 Western Blot analysis of CHD4 expression in HeLa NE. 100 μg Log in to see 8 to 11 Days
$450.00
Details
Human Rabbit Un-conjugated ICC, IF, IP, WB IP Image Immunoprecipitation of CHD4 protein from 293T whole cell extracts using 5 μg of CHD4 antibody, Western blot analysis was performed using CHD4 antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent. WB Image Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 5% SDS PAGE antibody diluted at 1:1000 100 μL Log in to see 1 to 2 Days
$358.60
Details
Bat Rabbit Un-conjugated IHC (p), WB Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) 50 μg Log in to see 7 to 9 Days
$727.83
Details
Human Mouse Un-conjugated IHC (p), ELISA, WB Immunoperoxidase of monoclonal antibody to CHD4 on formalin-fixed paraffin-embedded human transitional cell carcinoma. [antibody concentration 3 ug/ml] CHD4 monoclonal antibody (M01), clone 4H4 Western Blot analysis of CHD4 expression in Hela S3 NE . 100 μg Log in to see 11 to 12 Days
$440.00
Details
Human Rabbit Un-conjugated IP, PLA, WB Western Blot and Immunoprecipitation: CHD4/Mi2 beta Antibody [ABIN252858] - Detection of Human and Mouse CHD4/Mi2 beta by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB, 1 mg for IP, 20% of IP loaded), 293T (T, 50 mcg) and mouse NIH3T3 (M, 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CHD4/Mi2 beta antibody ABIN252858 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. CHD4/Mi2 beta was also immunoprecipitated by rabbit anti-CHD4/Mi2 beta antibodies ABIN252857 and ABIN252859, which recognize other epitopes. . Detection: Chemiluminescence with exposure times of 10 seconds (A and B). Proximity Ligation Assay: CHD4 Antibody [ABIN2528580] - image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red). 0.1 mL Log in to see 7 to 9 Days
$556.31
Details
Human Rabbit Un-conjugated IP, WB Western Blot and Immunoprecipitation: CHD4/Mi2 beta Antibody [ABIN252859] - Detection of Human and Mouse CHD4/Mi2 beta by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB, 1 mg for IP, 20% of IP loaded), 293T (T, 50 mcg) and mouse NIH3T3 (M, 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CHD4/Mi2 beta antibody ABIN252859 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. CHD4/Mi2 beta was also immunoprecipitated by rabbit anti-CHD4/Mi2 beta antibodies ABIN252858 and another anti-CHD4/Mi2 beta, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A and B). 0.1 mL Log in to see 7 to 9 Days
$556.31
Details
Human Rabbit Un-conjugated IP, WB Western Blot and Immunoprecipitation: CHD4/Mi2 beta Antibody [ABIN252857] - Detection of Human and Mouse CHD4/Mi2 beta by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB, 1 mg for IP, 20% of IP loaded), 293T (T, 50 mcg) and mouse NIH3T3 (M, 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CHD4/Mi2 beta antibody ABIN252857 used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. CHD4/Mi2 beta was also immunoprecipitated by rabbit anti-CHD4/Mi2 beta antibodies ABIN252858 and ABIN252859, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A and B). Western Blot: CHD4 Antibody - Detection of Human CHD4/Mi2 beta by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHD4/Mi2 beta antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. 100 μL Log in to see 7 to 9 Days
$556.31
Details

CHD4 Antibodies by Reactivity, Application, Clonality and Conjugate

Attributes Applications Host Clonality Conjugate
Human , , , ,
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Rat (Rattus)


Mouse (Murine) , , ,
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Top referenced anti-CHD4 Antibodies

  1. Human Polyclonal CHD4 Primary Antibody for ICC, IF - ABIN2668534 : Curtis, Griffin: The chromatin-remodeling enzymes BRG1 and CHD4 antagonistically regulate vascular Wnt signaling. in Molecular and cellular biology 2012 (PubMed)
    Show all 4 Pubmed References

  2. Human Polyclonal CHD4 Primary Antibody for IP, PLA - ABIN252858 : Ma, Lin, Bartolomei, Schultz: Metastasis tumor antigen 2 (MTA2) is involved in proper imprinted expression of H19 and Peg3 during mouse preimplantation development. in Biology of reproduction 2010 (PubMed)

  3. Human Polyclonal CHD4 Primary Antibody for IP, WB - ABIN252859 : Gu, Hu, Ebrahem, Crabb, Mahfouz, Radivoyevitch, Crabb, Saunthararajah: Runx1 regulation of Pu.1 corepressor/coactivator exchange identifies specific molecular targets for leukemia differentiation therapy. in The Journal of biological chemistry 2014 (PubMed)

  4. Human Polyclonal CHD4 Primary Antibody for IP, WB - ABIN252857 : Shakya, Kang, Chumley, Williams, Tantin: Oct1 is a switchable, bipotential stabilizer of repressed and inducible transcriptional states. in The Journal of biological chemistry 2011 (PubMed)

  5. Human Monoclonal CHD4 Primary Antibody for IHC (p), ELISA - ABIN560357 : Nishibuchi, Shibata, Hayakawa, Hayakawa, Ohtani, Sinmyozu, Tagami, Nakayama: Physical and functional interactions between the histone H3K4 demethylase KDM5A and the nucleosome remodeling and deacetylase (NuRD) complex. in The Journal of biological chemistry 2014 (PubMed)

  6. Fruit Fly (Drosophila melanogaster) Monoclonal CHD4 Primary Antibody for IP, WB - ABIN2668526 : Murawska, Kunert, van Vugt, Längst, Kremmer, Logie, Brehm: dCHD3, a novel ATP-dependent chromatin remodeler associated with sites of active transcription. in Molecular and cellular biology 2008 (PubMed)

More Antibodies against CHD4 Interaction Partners

Human Chromodomain Helicase DNA Binding Protein 4 (CHD4) interaction partners

  1. RNA interference identifies CHD4 as an essential gene in regulating breast cancer growth.

  2. Data suggest TCF19 (show TCF19 Antibodies) interacts with histone 3 lysine 4 trimethylation through its plant homeodomain finger; TCF19 (show TCF19 Antibodies) expression appears to regulate gluconeogenesis in hepatocytes; TCF19 (show TCF19 Antibodies) interacts with CHD4 causing NuRD complex recruitment to gene promoters of enzymes involved in gluconeogenesis. (TCF19 (show TCF19 Antibodies) = transcription factor 19 (show TCF19 Antibodies); CHD4 = chromodomain helicase DNA binding protein 4; NuRD = nucleosome-remodeling-deacetylase)

  3. CHD3 (show CHD3 Antibodies) and CHD4 exhibit distinct nuclear localization patterns in unperturbed cells, revealing a subset of specific target genes.

  4. The findings identify that CHD4 deficiency preferentially impairs cell survival via increasing the level of p21 (show CDKN1A Antibodies).

  5. Mutation in CHD4 gene is associated with congenital heart defects.

  6. this work identifies CHD4 as an epigenetic coregulator of PAX3 (show PAX3 Antibodies)-FOXO1 (show FOXO1 Antibodies) activity, providing rational evidence for CHD4 as a potential therapeutic target in alveolar rhabdomyosarcoma.

  7. CHD4 recruits repressive chromatin proteins to sites of DNA damage repair, including DNA methyltransferases where it imposes de novo DNA methylation (show HELLS Antibodies). At TSGs, CHD4 retention helps maintain DNA hypermethylation-associated transcriptional silencing.

  8. this study identifies the Chd4-Tbx3 (show TBX3 Antibodies) axis in controlling ESC fate and a role of H2A.Z (show H2AFZ Antibodies) in maintaining the stability of Chd4 proteins.

  9. report provides evidence for the role of CHD4 in human development and expands an increasingly recognized group of Mendelian disorders involving chromatin remodeling and modification

  10. complex lacking CHD4 that has HDAC (show HDAC3 Antibodies) activity can exist as a stable species. The addition of recombinant CHD4 to this nucleosome deacetylase complex reconstitutes a NuRD complex with nucleosome remodeling activity.

Mouse (Murine) Chromodomain Helicase DNA Binding Protein 4 (CHD4) interaction partners

  1. RNA interference identifies CHD4 as an essential gene in regulating breast cancer growth.

  2. Chd4 mutant embryos died before birth and exhibited severe edema, blood-filled lymphatics, and liver hemorrhage. CHD4-deficient embryos developed normal lymphovenous (LV) valves, which regulate the return of lymph to the blood circulation; however, these valves lacked the fibrin-rich thrombi that prevent blood from entering the lymphatic system.

  3. This secreted effector, toxoplasma inhibitor of STAT1 (show STAT1 Antibodies)-dependent transcription (TgIST), translocates to the host cell nucleus, where it recruits mouse Mi-2/NuRD to STAT1 (show STAT1 Antibodies)-dependent promoters, resulting in altered chromatin and blocked transcription.

  4. this study shows that the chromatin remodeler Mi-2beta controls epidermal homeostasis by regulating the genes involved in keratinocyte and immune-cell activation to maintain an inactive state

  5. preservation of the identity of the two striated (show NSDHL Antibodies) muscle types depends on epigenetic repression of the alternate lineage gene program by the chromatin remodeling complex Chd4/NuRD.

  6. complex lacking CHD4 that has HDAC (show HDAC3 Antibodies) activity can exist as a stable species. The addition of recombinant CHD4 to this nucleosome deacetylase complex reconstitutes a NuRD complex with nucleosome remodeling activity.

  7. CHD4 depletion modulates expression of acute myeloid leukemia (show BCL11A Antibodies) cell genes that regulate tumor formation in vivo and colony formation in vitro.

  8. CHD4 allows cells to undertake lineage commitment in vivo by modulating the frequency with which lineage-specification genes are expressed.

  9. Chromatin immunoprecipitation assays showed that CHD4-containing NuRD complexes directly bound the promoters of these genes in endothelial cells

  10. Chd4, interacting with Hdac1 (show HDAC1 Antibodies)/2, cooperates with its related proteins Kap1 (show TRIM28 Antibodies) and Cbx1 (show CBX1 Antibodies) to bind at -207/-148 of the Sox9 (show SOX9 Antibodies) promoter.

Xenopus laevis Chromodomain Helicase DNA Binding Protein 4 (CHD4) interaction partners

  1. CHD4 functions as a MAP required for MT stabilization and is involved in generating spindle bipolarity.

CHD4 Antigen Profile

Protein Summary

The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein.

Gene names and symbols associated with CHD4

  • chromodomain helicase DNA binding protein 4 (CHD4) antibody
  • chromodomain helicase DNA binding protein 4a (chd4a) antibody
  • chromodomain helicase DNA binding protein 4b (chd4b) antibody
  • chromodomain helicase DNA binding protein 4 (chd4) antibody
  • chromodomain helicase DNA binding protein 4 (Chd4) antibody
  • chromodomain helicase DNA binding protein 4 L homeolog (chd4.L) antibody
  • 9530019N15Rik antibody
  • AA617397 antibody
  • b230399n07 antibody
  • BC005710 antibody
  • CHD4 antibody
  • D6Ertd380e antibody
  • im:7143343 antibody
  • MGC145363 antibody
  • Mi-2 antibody
  • Mi-2b antibody
  • Mi-2beta antibody
  • Mi2-BETA antibody
  • mKIAA4075 antibody
  • si:ch211-51m24.3 antibody
  • si:ch211-57k11.10 antibody
  • wu:fb44b12 antibody
  • wu:fd12d03 antibody

Protein level used designations for CHD4

chromodomain helicase DNA binding protein 4 , chromodomain-helicase-DNA-binding protein 4 , ATP-dependent helicase CHD4 , CHD-4 , Mi-2 autoantigen 218 kDa protein , Mi-2 autoantigen , Mi-2 beta

GENE ID SPECIES
418278 Gallus gallus
451788 Pan troglodytes
558344 Danio rerio
560622 Danio rerio
100059817 Equus caballus
100124711 Xenopus (Silurana) tropicalis
100515610 Sus scrofa
100539798 Meleagris gallopavo
100566510 Anolis carolinensis
1108 Homo sapiens
117535 Rattus norvegicus
107932 Mus musculus
380196 Xenopus laevis
477714 Canis lupus familiaris
506402 Bos taurus
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