anti-Chromodomain Helicase DNA Binding Protein 4 (CHD4) Antibodies

Human Chromodomain Helicase DNA Binding Protein 4 (CHD4) interaction partners

1. CHD4 is essential for maintenance of childhood AML and the LICs that are responsible for the emergence and development of the disease. Our data indicate that the importance of CHD4 in childhood AML may be mediated in part by promoting the expression of the MYC oncogene and its target genes.

2. Alterations of EZH2, KMT2C, and CHD4 at genetic level or protein level could perturb epigenetic program, leading to malignant transformation in glioma.

3. that CHD4 is not only a potential prognostic biomarker for triple-negative breast cancer (TNBC) patient survival, but is also a powerful candidate in the development of new anti-cancer agents in TNBC

4. the N-terminal part of CHD4 interacts with an unstructured A-rich region in promoter and pre-rRNA antisense "), a long noncoding RNA that is transcribed in an orientation antisense to pre-rRNA

5. RNA interference identifies CHD4 as an essential gene in regulating breast cancer growth.

6. Data suggest TCF19 interacts with histone 3 lysine 4 trimethylation through its plant homeodomain finger; TCF19 expression appears to regulate gluconeogenesis in hepatocytes; TCF19 interacts with CHD4 causing NuRD complex recruitment to gene promoters of enzymes involved in gluconeogenesis. (TCF19 = transcription factor 19; CHD4 = chromodomain helicase DNA binding protein 4; NuRD = nucleosome-remodeling-deacetylase)

7. CHD3 and CHD4 exhibit distinct nuclear localization patterns in unperturbed cells, revealing a subset of specific target genes.

8. The findings identify that CHD4 deficiency preferentially impairs cell survival via increasing the level of p21.

9. Mutation in CHD4 gene is associated with congenital heart defects.

10. this work identifies CHD4 as an epigenetic coregulator of PAX3-FOXO1 activity, providing rational evidence for CHD4 as a potential therapeutic target in alveolar rhabdomyosarcoma.

11. CHD4 recruits repressive chromatin proteins to sites of DNA damage repair, including DNA methyltransferases where it imposes de novo DNA methylation. At TSGs, CHD4 retention helps maintain DNA hypermethylation-associated transcriptional silencing.

12. this study identifies the Chd4-Tbx3 axis in controlling ESC fate and a role of H2A.Z in maintaining the stability of Chd4 proteins.

13. report provides evidence for the role of CHD4 in human development and expands an increasingly recognized group of Mendelian disorders involving chromatin remodeling and modification

14. complex lacking CHD4 that has HDAC activity can exist as a stable species. The addition of recombinant CHD4 to this nucleosome deacetylase complex reconstitutes a NuRD complex with nucleosome remodeling activity.

15. CHD4 plays a pivotal role in chemoresistance and the maintenance of stemness in liver cancer stem cells and is therefore a good target for the eradication of hepatocellular carcinoma.

16. Acetyltransferase p300 collaborates with chromodomain helicase DNA-binding protein 4 (CHD4) to facilitate DNA double-strand break repair

17. Specifically, methyl-CpG-binding domain protein 2 (MBD2) is revealed to be recruited to DNA damage sites after laser microirradiation, which was mediated through MBD domain and MBD2 C-terminus.

18. these data build on our understanding of how CHD4-NuRD acts to regulate gene expression and participates in the DNA-damage response.

19. CHD4 depletion modulates expression of acute myeloid leukemia cell genes that regulate tumor formation in vivo and colony formation in vitro.

20. Also discovered a novel causative role for CHD4, a helicase involved in the histone deacetylase complex that is associated with poor clinical outcome.

Mouse (Murine) Chromodomain Helicase DNA Binding Protein 4 (CHD4) interaction partners

1. findings reveal that the CHD4/NuRD complex regulates neural differentiation of ESCs by down-regulating p53.

2. results reveal that ADNP, via the recruitment of HP1 and CHD4, regulates the expression of genes that are crucial for maintaining distinct cellular states and assures accurate cell fate decisions upon external cues

3. Direct CHD4-mediated repression of skeletal and smooth muscle myofibril isoforms is required for normal cardiac sarcomere formation, function, and embryonic survival early in gestation. Unique CHD4 binding sites are found in smooth muscle myosin heavy chain, fast skeletal alpha-actin, and the fast skeletal troponin complex genes.

4. RNA interference identifies CHD4 as an essential gene in regulating breast cancer growth.

5. Chd4 mutant embryos died before birth and exhibited severe edema, blood-filled lymphatics, and liver hemorrhage. CHD4-deficient embryos developed normal lymphovenous (LV) valves, which regulate the return of lymph to the blood circulation; however, these valves lacked the fibrin-rich thrombi that prevent blood from entering the lymphatic system.

6. This secreted effector, toxoplasma inhibitor of STAT1-dependent transcription (TgIST), translocates to the host cell nucleus, where it recruits mouse Mi-2/NuRD to STAT1-dependent promoters, resulting in altered chromatin and blocked transcription.

7. this study shows that the chromatin remodeler Mi-2beta controls epidermal homeostasis by regulating the genes involved in keratinocyte and immune-cell activation to maintain an inactive state

8. preservation of the identity of the two striated muscle types depends on epigenetic repression of the alternate lineage gene program by the chromatin remodeling complex Chd4/NuRD.

9. complex lacking CHD4 that has HDAC activity can exist as a stable species. The addition of recombinant CHD4 to this nucleosome deacetylase complex reconstitutes a NuRD complex with nucleosome remodeling activity.

10. CHD4 depletion modulates expression of acute myeloid leukemia cell genes that regulate tumor formation in vivo and colony formation in vitro.

11. CHD4 allows cells to undertake lineage commitment in vivo by modulating the frequency with which lineage-specification genes are expressed.

12. Chromatin immunoprecipitation assays showed that CHD4-containing NuRD complexes directly bound the promoters of these genes in endothelial cells

13. Chd4, interacting with Hdac1/2, cooperates with its related proteins Kap1 and Cbx1 to bind at -207/-148 of the Sox9 promoter.

14. NuRD negatively regulates TIP5 expression, thereby inhibiting ribosomal DNA (rDNA) methylation and maintaining demethylation state of rDNA promoters.

15. The chromodomain helicase DNA-binding protein 4 (Chd4) is a critical interaction partner of Ezh2 required specifically for Polycomb group (PcG)-mediated suppression of the key astrogenic marker gene GFAP.

16. Gata3/Chd4 forms functionally distinct complexes, which mediate both positive and negative gene regulation to facilitate Th2 cell differentiation.

17. In this study, we demonstrate that Mi2beta is required for gamma-globin gene silencing during adult definitive erythropoiesis

18. Concomitant deletion of Chd4 and Brg1 rescued vascular abnormalities seen in Brg1 mutant yolk sacs to the same extent as LiCl treatment.

19. activity of the Mi-2beta nucleosome-remodeling and histone-deacetylase (NuRD) complex was controlled by the Ikaros family of lymphoid lineage-determining proteins.

20. downregulated expression in T cells following treatment with bis(tri-n-butylin)oxide (TBTO), an immunotoxic organotin

Xenopus laevis Chromodomain Helicase DNA Binding Protein 4 (CHD4) interaction partners

1. CHD4 functions as a MAP required for MT stabilization and is involved in generating spindle bipolarity.