anti-Chromodomain Helicase DNA Binding Protein 4 (CHD4) Antibodies

The product of CHD4 belongs to the SNF2/RAD54 helicase family. Additionally we are shipping CHD4 Kits (7) and CHD4 Proteins (7) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
CHD4 1108 Q14839
CHD4 117535  
CHD4 107932 Q6PDQ2
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Top anti-CHD4 Antibodies at antibodies-online.com

Showing 10 out of 76 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Delivery Price Details
Cow Rabbit Un-conjugated WB WB Suggested Anti-CHD4 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:12500 Positive Control: OVCAR-3 cell lysate There is BioGPS gene expression data showing that CHD4 is expressed in OVCAR3 100 μL 2 to 3 Days
$289.00
Details
Human Rabbit Un-conjugated IC, IF, IHC, WB Immunofluorescent analysis of CHD4 staining in HEK293 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody Immunohistochemical analysis of CHD4 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with 200 μL 13 to 14 Days
$487.50
Details
Human Rabbit Un-conjugated IF, IHC, ELISA Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using CHD4 Antibody. The picture on the right is treated with the synthesized peptide. Immunofluorescence analysis of HepG2 cells, using CHD4 Antibody. The picture on the right is treated with the synthesized peptide. 100 μg 2 to 3 Days
$302.50
Details
Human Rabbit Un-conjugated ICC, IF, IP, WB IP Image Immunoprecipitation of CHD4 protein from 293T whole cell extracts using 5 μg of CHD4 antibody, Western blot analysis was performed using CHD4 antibody, EasyBlot anti-Rabbit IgG  was used as a secondary reagent. WB Image Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 5% SDS PAGE antibody diluted at 1:1000 100 μL 3 to 4 Days
$466.18
Details
Bat Rabbit Un-conjugated IHC, IHC (p), WB Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) Anti-CHD4 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other form ... 100 μL 11 to 14 Days
$727.83
Details
Human Mouse Un-conjugated IHC (p), ELISA, WB Immunoperoxidase of monoclonal antibody to CHD4 on formalin-fixed paraffin-embedded human transitional cell carcinoma. [antibody concentration 3 ug/ml] CHD4 monoclonal antibody (M01), clone 4H4 Western Blot analysis of CHD4 expression in Hela S3 NE . 100 μg 11 to 12 Days
$364.00
Details
Human Mouse Un-conjugated ELISA, IHC, IHC (p), WB Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE) Anti-CHD4 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. 50 μg 11 to 14 Days
$742.50
Details
Human Rabbit Un-conjugated IF, IHC, WB 100 μL 11 to 13 Days
$476.15
Details
Human Rabbit Un-conjugated ICC, IF, IHC, WB Mi-2 beta antibody tested by immunofluorescence. Top left: HeLa cells stained with Mi-2 beta antibody (1:1,000). Top right: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom left: Same cells stained with DAPI. Bottom right: Merge of all 3 images. Mi-2 beta antibody tested by Western blot. Whole-cell extract of mouse splenocytes stained with Mi-2 beta antibody (1:1,1000). 100 μL 1 to 2 Days
$507.50
Details
Human Mouse Un-conjugated ELISA, WB   100 μL Available
$363.46
Details

Top referenced anti-CHD4 Antibodies

  1. Human Polyclonal CHD4 Primary Antibody for ICC, IF - ABIN6579173 : Curtis, Griffin: The chromatin-remodeling enzymes BRG1 and CHD4 antagonistically regulate vascular Wnt signaling. in Molecular and cellular biology 2012 (PubMed)
    Show all 4 Pubmed References

  2. Human Monoclonal CHD4 Primary Antibody for IHC (p), ELISA - ABIN560357 : Nishibuchi, Shibata, Hayakawa, Hayakawa, Ohtani, Sinmyozu, Tagami, Nakayama: Physical and functional interactions between the histone H3K4 demethylase KDM5A and the nucleosome remodeling and deacetylase (NuRD) complex. in The Journal of biological chemistry 2014 (PubMed)

  3. Human Polyclonal CHD4 Primary Antibody for IP, WB - ABIN252857 : Shakya, Kang, Chumley, Williams, Tantin: Oct1 is a switchable, bipotential stabilizer of repressed and inducible transcriptional states. in The Journal of biological chemistry 2011 (PubMed)

  4. Human Polyclonal CHD4 Primary Antibody for IP, WB - ABIN252859 : Gu, Hu, Ebrahem, Crabb, Mahfouz, Radivoyevitch, Crabb, Saunthararajah: Runx1 regulation of Pu.1 corepressor/coactivator exchange identifies specific molecular targets for leukemia differentiation therapy. in The Journal of biological chemistry 2014 (PubMed)

  5. Human Polyclonal CHD4 Primary Antibody for IP, PLA - ABIN252858 : Ma, Lin, Bartolomei, Schultz: Metastasis tumor antigen 2 (MTA2) is involved in proper imprinted expression of H19 and Peg3 during mouse preimplantation development. in Biology of reproduction 2010 (PubMed)

  6. Fruit Fly (Drosophila melanogaster) Monoclonal CHD4 Primary Antibody for IP, WB - ABIN2668526 : Murawska, Kunert, van Vugt, Längst, Kremmer, Logie, Brehm: dCHD3, a novel ATP-dependent chromatin remodeler associated with sites of active transcription. in Molecular and cellular biology 2008 (PubMed)

  7. Human Monoclonal CHD4 Primary Antibody for ChIP, CyTOF - ABIN4297878 : Yen, Sharma, Cols, Lau, Chaudhry, Chowdhury, Yewdell, Vaidyanathan, Sun, Coffre, Pucella, Chen, Jasin, Sun, Rudensky, Koralov, Chaudhuri: Distinct Requirements of CHD4 during B Cell Development and Antibody Response. in Cell reports 2019 (PubMed)

More Antibodies against CHD4 Interaction Partners

Human Chromodomain Helicase DNA Binding Protein 4 (CHD4) interaction partners

  1. CHD4 is essential for maintenance of childhood AML and the LICs that are responsible for the emergence and development of the disease. Our data indicate that the importance of CHD4 in childhood AML may be mediated in part by promoting the expression of the MYC oncogene and its target genes.

  2. Alterations of EZH2, KMT2C, and CHD4 at genetic level or protein level could perturb epigenetic program, leading to malignant transformation in glioma.

  3. that CHD4 is not only a potential prognostic biomarker for triple-negative breast cancer (TNBC) patient survival, but is also a powerful candidate in the development of new anti-cancer agents in TNBC

  4. the N-terminal part of CHD4 interacts with an unstructured A-rich region in promoter and pre-rRNA antisense "), a long noncoding RNA that is transcribed in an orientation antisense to pre-rRNA

  5. RNA interference identifies CHD4 as an essential gene in regulating breast cancer growth.

  6. Data suggest TCF19 interacts with histone 3 lysine 4 trimethylation through its plant homeodomain finger; TCF19 expression appears to regulate gluconeogenesis in hepatocytes; TCF19 interacts with CHD4 causing NuRD complex recruitment to gene promoters of enzymes involved in gluconeogenesis. (TCF19 = transcription factor 19; CHD4 = chromodomain helicase DNA binding protein 4; NuRD = nucleosome-remodeling-deacetylase)

  7. CHD3 and CHD4 exhibit distinct nuclear localization patterns in unperturbed cells, revealing a subset of specific target genes.

  8. The findings identify that CHD4 deficiency preferentially impairs cell survival via increasing the level of p21.

  9. Mutation in CHD4 gene is associated with congenital heart defects.

  10. this work identifies CHD4 as an epigenetic coregulator of PAX3-FOXO1 activity, providing rational evidence for CHD4 as a potential therapeutic target in alveolar rhabdomyosarcoma.

  11. CHD4 recruits repressive chromatin proteins to sites of DNA damage repair, including DNA methyltransferases where it imposes de novo DNA methylation. At TSGs, CHD4 retention helps maintain DNA hypermethylation-associated transcriptional silencing.

  12. this study identifies the Chd4-Tbx3 axis in controlling ESC fate and a role of H2A.Z in maintaining the stability of Chd4 proteins.

  13. report provides evidence for the role of CHD4 in human development and expands an increasingly recognized group of Mendelian disorders involving chromatin remodeling and modification

  14. complex lacking CHD4 that has HDAC activity can exist as a stable species. The addition of recombinant CHD4 to this nucleosome deacetylase complex reconstitutes a NuRD complex with nucleosome remodeling activity.

  15. CHD4 plays a pivotal role in chemoresistance and the maintenance of stemness in liver cancer stem cells and is therefore a good target for the eradication of hepatocellular carcinoma.

  16. Acetyltransferase p300 collaborates with chromodomain helicase DNA-binding protein 4 (CHD4) to facilitate DNA double-strand break repair

  17. Specifically, methyl-CpG-binding domain protein 2 (MBD2) is revealed to be recruited to DNA damage sites after laser microirradiation, which was mediated through MBD domain and MBD2 C-terminus.

  18. these data build on our understanding of how CHD4-NuRD acts to regulate gene expression and participates in the DNA-damage response.

  19. CHD4 depletion modulates expression of acute myeloid leukemia cell genes that regulate tumor formation in vivo and colony formation in vitro.

  20. Also discovered a novel causative role for CHD4, a helicase involved in the histone deacetylase complex that is associated with poor clinical outcome.

Mouse (Murine) Chromodomain Helicase DNA Binding Protein 4 (CHD4) interaction partners

  1. findings reveal that the CHD4/NuRD complex regulates neural differentiation of ESCs by down-regulating p53.

  2. results reveal that ADNP, via the recruitment of HP1 and CHD4, regulates the expression of genes that are crucial for maintaining distinct cellular states and assures accurate cell fate decisions upon external cues

  3. Direct CHD4-mediated repression of skeletal and smooth muscle myofibril isoforms is required for normal cardiac sarcomere formation, function, and embryonic survival early in gestation. Unique CHD4 binding sites are found in smooth muscle myosin heavy chain, fast skeletal alpha-actin, and the fast skeletal troponin complex genes.

  4. RNA interference identifies CHD4 as an essential gene in regulating breast cancer growth.

  5. Chd4 mutant embryos died before birth and exhibited severe edema, blood-filled lymphatics, and liver hemorrhage. CHD4-deficient embryos developed normal lymphovenous (LV) valves, which regulate the return of lymph to the blood circulation; however, these valves lacked the fibrin-rich thrombi that prevent blood from entering the lymphatic system.

  6. This secreted effector, toxoplasma inhibitor of STAT1-dependent transcription (TgIST), translocates to the host cell nucleus, where it recruits mouse Mi-2/NuRD to STAT1-dependent promoters, resulting in altered chromatin and blocked transcription.

  7. this study shows that the chromatin remodeler Mi-2beta controls epidermal homeostasis by regulating the genes involved in keratinocyte and immune-cell activation to maintain an inactive state

  8. preservation of the identity of the two striated muscle types depends on epigenetic repression of the alternate lineage gene program by the chromatin remodeling complex Chd4/NuRD.

  9. complex lacking CHD4 that has HDAC activity can exist as a stable species. The addition of recombinant CHD4 to this nucleosome deacetylase complex reconstitutes a NuRD complex with nucleosome remodeling activity.

  10. CHD4 depletion modulates expression of acute myeloid leukemia cell genes that regulate tumor formation in vivo and colony formation in vitro.

  11. CHD4 allows cells to undertake lineage commitment in vivo by modulating the frequency with which lineage-specification genes are expressed.

  12. Chromatin immunoprecipitation assays showed that CHD4-containing NuRD complexes directly bound the promoters of these genes in endothelial cells

  13. Chd4, interacting with Hdac1/2, cooperates with its related proteins Kap1 and Cbx1 to bind at -207/-148 of the Sox9 promoter.

  14. NuRD negatively regulates TIP5 expression, thereby inhibiting ribosomal DNA (rDNA) methylation and maintaining demethylation state of rDNA promoters.

  15. The chromodomain helicase DNA-binding protein 4 (Chd4) is a critical interaction partner of Ezh2 required specifically for Polycomb group (PcG)-mediated suppression of the key astrogenic marker gene GFAP.

  16. Gata3/Chd4 forms functionally distinct complexes, which mediate both positive and negative gene regulation to facilitate Th2 cell differentiation.

  17. In this study, we demonstrate that Mi2beta is required for gamma-globin gene silencing during adult definitive erythropoiesis

  18. Concomitant deletion of Chd4 and Brg1 rescued vascular abnormalities seen in Brg1 mutant yolk sacs to the same extent as LiCl treatment.

  19. activity of the Mi-2beta nucleosome-remodeling and histone-deacetylase (NuRD) complex was controlled by the Ikaros family of lymphoid lineage-determining proteins.

  20. downregulated expression in T cells following treatment with bis(tri-n-butylin)oxide (TBTO), an immunotoxic organotin

Xenopus laevis Chromodomain Helicase DNA Binding Protein 4 (CHD4) interaction partners

  1. CHD4 functions as a MAP required for MT stabilization and is involved in generating spindle bipolarity.

CHD4 Antigen Profile

Protein Summary

The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein.

Gene names and symbols associated with CHD4

  • chromodomain helicase DNA binding protein 4 (CHD4) antibody
  • chromodomain helicase DNA binding protein 4a (chd4a) antibody
  • chromodomain helicase DNA binding protein 4b (chd4b) antibody
  • chromodomain helicase DNA binding protein 4 (chd4) antibody
  • chromodomain helicase DNA binding protein 4 (Chd4) antibody
  • chromodomain helicase DNA binding protein 4 L homeolog (chd4.L) antibody
  • 9530019N15Rik antibody
  • AA617397 antibody
  • b230399n07 antibody
  • BC005710 antibody
  • CHD4 antibody
  • D6Ertd380e antibody
  • im:7143343 antibody
  • MGC145363 antibody
  • Mi-2 antibody
  • Mi-2b antibody
  • Mi-2beta antibody
  • Mi2-BETA antibody
  • mKIAA4075 antibody
  • si:ch211-51m24.3 antibody
  • si:ch211-57k11.10 antibody
  • wu:fb44b12 antibody
  • wu:fd12d03 antibody

Protein level used designations for CHD4

chromodomain helicase DNA binding protein 4 , chromodomain-helicase-DNA-binding protein 4 , ATP-dependent helicase CHD4 , CHD-4 , Mi-2 autoantigen 218 kDa protein , Mi-2 autoantigen , Mi-2 beta

GENE ID SPECIES
418278 Gallus gallus
451788 Pan troglodytes
558344 Danio rerio
560622 Danio rerio
100059817 Equus caballus
100124711 Xenopus (Silurana) tropicalis
100515610 Sus scrofa
100539798 Meleagris gallopavo
100566510 Anolis carolinensis
1108 Homo sapiens
117535 Rattus norvegicus
107932 Mus musculus
380196 Xenopus laevis
477714 Canis lupus familiaris
506402 Bos taurus
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