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CHD4 antibody (C-Term)

CHD4 Reactivity: Synthetic WB, ELISA, IF, FACS, Coat, IHC (f) Host: Mouse Monoclonal 3F2-4 unconjugated
Catalog No. ABIN6939119
  • Target See all CHD4 Antibodies
    CHD4 (Chromodomain Helicase DNA Binding Protein 4 (CHD4))
    Binding Specificity
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 38
    • 24
    • 8
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Synthetic
    Host
    • 30
    • 8
    • 1
    Mouse
    Clonality
    • 30
    • 9
    Monoclonal
    Conjugate
    • 28
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CHD4 antibody is un-conjugated
    Application
    • 23
    • 16
    • 11
    • 10
    • 9
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Specificity
    CHD4/Mi-2b is a component of the nucleosome remodeling and deacetylase (NuRD) complex which is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex function primarily in transcriptional repression. Accumulating evidence indicates that NuRD may regulate the transcription of specific genes by interacting with specific transcriptional factors. In addition, the NuRD complex may also participate in genome-wide transcriptional regulation through an association with histone tails.
    Cross-Reactivity (Details)
    Human, Mouse,
    Purification
    200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.
    Immunogen
    Synthetic peptide (N- and C- terminal peptides were used for immunisation: 1. ASGLGSPSPCSAGSEEEDM & 2. CSRLANRAPEPPPQQVAQQQ) - which peptide the antibody recognises has not been tested.
    Clone
    3F2-4
    Isotype
    IgG2a
    Top Product
    Discover our top product CHD4 Primary Antibody
  • Application Notes

    Positive Control: HeLa whole cell lysate or human placenta tissue lysate. Human breast carcinoma.

    Known Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL)Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.
    Expiry Date
    24 months
  • Target
    CHD4 (Chromodomain Helicase DNA Binding Protein 4 (CHD4))
    Alternative Name
    CHD4 (CHD4 Products)
    Synonyms
    CHD4 antibody, im:7143343 antibody, si:ch211-51m24.3 antibody, si:ch211-57k11.10 antibody, wu:fb44b12 antibody, wu:fd12d03 antibody, MGC145363 antibody, Mi-2b antibody, Mi2-BETA antibody, Mi-2 antibody, 9530019N15Rik antibody, AA617397 antibody, BC005710 antibody, D6Ertd380e antibody, Mi-2beta antibody, mKIAA4075 antibody, b230399n07 antibody, chromodomain helicase DNA binding protein 4 antibody, chromodomain helicase DNA binding protein 4a antibody, chromodomain helicase DNA binding protein 4b antibody, chromodomain helicase DNA binding protein 4 L homeolog antibody, CHD4 antibody, chd4a antibody, chd4b antibody, chd4 antibody, Chd4 antibody, chd4.L antibody
    Background
    ATP dependent helicase CHD4, Chromodomain helicase DNA binding protein 4, Mi 2 autoantigen 218 kDa protein, Mi 2b, Mi2 beta,CHD4
    Cellular localisation: Nucleus and cytoplasm.
    Molecular Weight
    238kDa
    Gene ID
    1108, 162233
    UniProt
    Q14839
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