anti-Cyclin G Associated Kinase (GAK) Antibodies

In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Additionally we are shipping GAK Kits (18) and GAK Proteins (5) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
GAK 2580 O14976
GAK 231580 Q99KY4
GAK 81659 P97874
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Top anti-GAK Antibodies at antibodies-online.com

Showing 10 out of 107 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Delivery Price Details
Human Rabbit Un-conjugated IC, IF, IHC, WB Immunofluorescent analysis of GAK staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Immunohistochemical analysis of GAK staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. 200 μL 13 to 14 Days
$487.50
Details
Human Rabbit Un-conjugated ELISA, WB Western blot analysis of extracts from 293 cells, using GAK Antibody. The lane on the right is treated with the synthesized peptide. 100 μg 2 to 3 Days
$302.50
Details
Human Rabbit Un-conjugated EIA, IHC (p), WB 0.4 mL 6 to 8 Days
$484.00
Details
Human Rabbit Un-conjugated IHC (p), WB Western blot analysis of GAK (arrow) using GAK Antibody (C-term) (ABIN392633). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the GAK gene (Lane 2) (Origene Technologies). Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma 400 μL 10 to 11 Days
$385.00
Details
Human Rabbit Un-conjugated EIA, IHC (p), WB 0.4 mL 6 to 8 Days
$484.00
Details
Human Rabbit Un-conjugated ELISA, IF, IHC, WB 100 μL Available
$363.46
Details
Human Rabbit Un-conjugated ELISA, IHC, IHC (p), WB Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) 50 μL 11 to 14 Days
$484.00
Details
Human Rabbit Un-conjugated ELISA, ICC, IF, WB Western blot analysis of extracts from mouse brain, using GAK Antibody. Lane 1 was treated with the blocking peptide. ABIN6266696 staining 293 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibody. 100 μL 11 to 12 Days
$390.77
Details
Human Mouse Un-conjugated IP, ELISA, WB GAK monoclonal antibody (M01), clone 4C10 Western Blot analysis of GAK expression in Hela S3 NE . Detection limit for recombinant GST tagged GAK is approximately 0.03ng/ml as a capture antibody. 100 μg 11 to 12 Days
$364.00
Details
Human Rabbit Un-conjugated ELISA, IF, IHC (p), WB 200 μL 12 to 14 Days
$438.90
Details

Top referenced anti-GAK Antibodies

  1. Human Polyclonal GAK Primary Antibody for IHC (p), ELISA - ABIN544224 : Kimura, Tsuruga, Yabuta, Endo, Nojima: Structure, expression, and chromosomal localization of human GAK. in Genomics 1997 (PubMed)
    Show all 3 Pubmed References

  2. Human Polyclonal GAK Primary Antibody for IHC (p), ELISA - ABIN544225 : Greener, Zhao, Nojima, Eisenberg, Greene: Role of cyclin G-associated kinase in uncoating clathrin-coated vesicles from non-neuronal cells. in The Journal of biological chemistry 2000 (PubMed)
    Show all 3 Pubmed References

More Antibodies against GAK Interaction Partners

Human Cyclin G Associated Kinase (GAK) interaction partners

  1. These findings indicate that the abnormal mitoses seen after silencing OIP5-AS1 were caused by an untimely rise in GAK levels and suggest that OIP5-AS1 suppresses cell proliferation at least in part by reducing GAK levels.

  2. that the multiple single nucleotide polymorphisms of GAK synergistically participate in the pathogenesis of sporadic Parkinson's disease through multiple pathways.

  3. We have demonstrated that siRNA-mediated GAK silencing causes marked cell cycle disruption resulting in a raised sub-G1 alongside decreased G1 and G2/M cell cycle phases. Dual FBXW7-GAK inhibition increases multipolar mitoses

  4. GAK gene expression is increased in Parkinson disease

  5. These results suggest that the c-Src_GAK_MCM axis plays an important role in cell cycle progression through control of the DNA replication licensing system.

  6. We filtered four OSCC genes including SERPINB9, SERPINE2, GAK, and HSP90B1 through the gene global prioritization score (P < 0.005).

  7. This meta-analysis suggests that GAK rs1564282 C/T polymorphism is associated with increased susceptibility to Parkinson's disease--{REVIEW}

  8. Data indicated that GBA and TMEM175/GAK significantly alter age at onset in PD.

  9. AAK1 and GAK are critical regulators of HCV entry that function in part by activating EGFR, AP2M1, and NUMB, and as the molecular targets underlying the antiviral effect of sunitinib and erlotinib, respectively.

  10. The effects of rs11248051 and rs1564282 variants of GAK, and the rs3129882 variant of HLA-DRA, were investigated in Parkinson's disease patients.

  11. GAK apo structure reveals a dimeric inactive state of the catalytic domain mediated by an unusual activation segment interaction.

  12. Cyclin-G-associated kinase is a binding partners of LRRK2, a candidate genes for risk for sporadic Parkinson disease, and part of a complex that promotes clearance of Golgi-derived vesicles through the autophagy-lysosome system.

  13. Dominant negative mutants of IRAK4 and GAK show strong apoptotic effects in A498 cells under anoxia.

  14. neither the CT, TT genotypes nor the minor allele T of single nucleotide polymorphism rs1564282 were associated with Parkinson's disease among the subjects from Taiwan and Singapore.

  15. rs11248060 significantly increasThis study found that the minor alleles of GAK rpopulationss1564282 and DGKQ e the risk of PD in Han Chinese .

  16. GAK as a regulator of dephosphorylation events under the control of the PP2A B'gamma subunit.

  17. study demonstrated that the rs1564282 variant in GAK (PARK17) increases the risk of Parkinson's disease in Han Chinese patients from mainland China

  18. cyclin G associated kinase is associated with Parkinson disease risk and suggest that cyclin G associated kinase and alpha-synuclein interact in a pathway involved in disease pathogenesis

  19. GAK is a new candidate for investigation in future studies.

  20. Osteosarcoma cell proliferation and survival are dependent on GAK.

Mouse (Murine) Cyclin G Associated Kinase (GAK) interaction partners

  1. PTEN-like domains of GAK and auxilin are not essential for Hsc70-dependent chaperoning and uncoating of clathrin, but depending on the tissue, these domains appear to increase the efficiency of these co-chaperones.

  2. GAK knockout causes senescence and centrosome duplication in MEF cells.

  3. These results suggest that inhibition of GAK by gefitinib may cause pulmonary alveolar dysfunction.

  4. GAK deletion blocks development and causes lethality in adult animals by disrupting clathrin-mediated endocytosis

GAK Antigen Profile

Protein Summary

In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5.

Gene names and symbols associated with GAK

  • cyclin G associated kinase (GAK) antibody
  • cyclin G associated kinase (gak) antibody
  • cyclin G associated kinase (Gak) antibody
  • D130045N16Rik antibody
  • DKFZp469F1313 antibody
  • DNAJ26 antibody
  • DNAJC26 antibody
  • gak antibody
  • MGC172462 antibody
  • si:dkeyp-1e8.1 antibody

Protein level used designations for GAK

cyclin G associated kinase , cyclin-G-associated kinase-like , auxilin-2 , cyclin-G-associated kinase , cyclin G-associated kinase

GENE ID SPECIES
100051853 Equus caballus
100171982 Pongo abelii
549104 Xenopus (Silurana) tropicalis
461048 Pan troglodytes
703406 Macaca mulatta
571705 Danio rerio
100482527 Ailuropoda melanoleuca
2580 Homo sapiens
231580 Mus musculus
81659 Rattus norvegicus
427291 Gallus gallus
479133 Canis lupus familiaris
511296 Bos taurus
101106688 Ovis aries
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