anti-Fanconi Anemia Group A Protein (FANCA) Antibodies

The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). Additionally we are shipping Fanconi Anemia Group A Protein Kits (4) and Fanconi Anemia Group A Protein Proteins (4) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
FANCA 2175 O15360
FANCA 14087 Q9JL70
FANCA 361435  
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Top anti-Fanconi Anemia Group A Protein Antibodies at antibodies-online.com

Showing 10 out of 116 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Cow Rabbit Un-conjugated WB WB Suggested Anti-FANCA Antibody Titration:  0.2-1 ug/ml  ELISA Titer:  1:62500  Positive Control:  Human Spleen 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Rabbit Un-conjugated IC, IF, IP, WB Immunofluorescent analysis of FANCA (pS1149) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Western blot analysis of FANCA (pS1149) expression in HepG2 EGF-treated (A), SP2/0 EGF-treated (B), H9C2 EGF-treated (C) whole cell lysates. 200 μL Log in to see 13 to 14 Days
$575.00
Details
Human Rabbit Un-conjugated ELISA, IHC, WB ABIN6269644 at 1/100 staining human heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary. ABIN6269644 at 1/100 staining rat heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary. 100 μL Log in to see 11 to 12 Days
$450.00
Details
Human Rabbit Un-conjugated IF, IHC, ELISA, WB Western blot analysis of extracts from HeLa cells treated with IGF 100ng/ml 10' and HuvEc cells treated with EGF 200ng/ml 30', using FANCA (Phospho-Ser1149) Antibody. The lane on the right is treated with the synthesized peptide. Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using FANCA (Phospho-Ser1149) Antibody. The picture on the right is treated with the synthesized peptide. 100 μg Log in to see 2 to 3 Days
$302.50
Details
Human Rabbit Un-conjugated IHC, ELISA Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using FANCA (Ab-1149) Antibody. The picture on the right is treated with the synthesized peptide. 100 μg Log in to see 2 to 3 Days
$302.50
Details
Human Rabbit Un-conjugated ELISA, WB Western blot using affinity purified anti-FANCA antibody shows detection of FANCA only in FANCA transfected GM6914 cell lysates.  No staining is seen in lysates prepared from FANCA (-/-) cells in the absence of FANCA transfection. Modified from Smogorzewska et al (2007) Cell 129, 289-301. Western blot using  affinity purified anti-FANCA antibody shows detection of a band at ~133 kDa (arrowhead) corres-ponding to FANCA in HeLa whole cell lysates.  The identity of the lower molecular weight bands is unknown but may represent breakdown products.   Approximately 35 µg of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed for 2 h at room temperature with the primary antibody diluted to 1:1,500. The membrane was washed and reacted with a 1:10,000 dilution of800 conjugated Gt-a-Rabbit IgG [H&L]  for 45 min at room temperature (800 nm channel, green).  Molecular weight estimation was made by comparison to prestained MW markers  indicated at left (700 nm channel, red). 800 fluorescence images were captured using the Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results. 100 μg Log in to see 5 to 7 Days
$598.50
Details
Human Rabbit Un-conjugated ELISA, IF, IHC, WB 100 μL Log in to see Available
$363.46
Details
Human Rabbit Un-conjugated ELISA, IHC, IHC (p) Human Uterus, Endometrium: Formalin-Fixed, Paraffin-Embedded (FFPE) Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) 50 μL Log in to see 11 to 14 Days
$484.00
Details
Cow Rabbit Un-conjugated WB 100 μL Log in to see 11 to 14 Days
$551.83
Details
Human Rabbit Un-conjugated ELISA, ICC, IF, IHC, WB Western blot analysis of Phospho-FANCA (Ser1149) using K562 whole cell lysates ABIN6278652 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibod 100 μL Log in to see 11 to 12 Days
$390.77
Details

Top referenced anti-Fanconi Anemia Group A Protein Antibodies

  1. Human Polyclonal FANCA Primary Antibody for IP, WB - ABIN5665644 : Hicks, Chute, Paulsen, Ragland, Howlett, Guéranger, Glover, Canman: Differential roles for DNA polymerases eta, zeta, and REV1 in lesion bypass of intrastrand versus interstrand DNA cross-links. in Molecular and cellular biology 2010 (PubMed)
    Show all 3 Pubmed References

  2. Human Polyclonal FANCA Primary Antibody for WB - ABIN251002 : Park, Ciccone, Beck, Hwang, Freie, Clapp, Lee: Oxidative stress/damage induces multimerization and interaction of Fanconi anemia proteins. in The Journal of biological chemistry 2004 (PubMed)

  3. Human Polyclonal FANCA Primary Antibody for ELISA, WB - ABIN129606 : Smogorzewska, Matsuoka, Vinciguerra, McDonald, Hurov, Luo, Ballif, Gygi, Hofmann, DAndrea, Elledge: Identification of the FANCI protein, a monoubiquitinated FANCD2 paralog required for DNA repair. in Cell 2007 (PubMed)

  4. Human Polyclonal FANCA Primary Antibody for ELISA, ICC - ABIN4309986 : Huard, Tremblay, Magron, Lévesque, Carreau: The Fanconi anemia pathway has a dual function in Dickkopf-1 transcriptional repression. in Proceedings of the National Academy of Sciences of the United States of America 2014 (PubMed)

More Antibodies against Fanconi Anemia Group A Protein Interaction Partners

Human Fanconi Anemia Group A Protein (FANCA) interaction partners

  1. Novel Variations of FANCA Gene Provokes Fanconi Anemia: Molecular Diagnosis in a Special Chinese Family.

  2. FANCG stimulates FANCA-mediated strand annealing and strand exchange.

  3. We report an extraordinarily high frequency of Fanconi anemia (FA) in a specific subgroup of azoospermic patients (7.1%). The screening for FANCA pathogenic variants in such patients has the potential to identify undiagnosed FA before the appearance of other severe clinical manifestations of the disease.

  4. variants at residues His913 and Arg951 are hypomorphic mutations. Consistent with these findings, the clinical phenotype of most of the patients carrying these mutations is mild. These data further support the recent finding that the Fanconi anemia proteins play a role in mitochondria, and open up possibilities for genotype/phenotype studies based on novel mitochondrial criteria.

  5. Mutations in the FANCA gene are associated with esophageal atresia and tracheoesophageal fistula in humans.

  6. the current study was the first to report a promoter region variation in the FANCA gene among women with breast cancer in Iran. The results of the present study confirmed the allelic variants in the FANCA promoter region as a tumor suppressor gene.

  7. we show that although FANCA S1088F protein properly localizes to the nucleus, it alters FANC complex function, enhances sensitivity to DNA damaging agents, and sensitizes cells to PARP inhibitors in vitro and in vivo.

  8. FancA amplification and overexpression appear to be crucial for radiotherapeutic failure in head and neck squamous cell carcinomas.

  9. High resolution melting curve analysis was used to screen FANCA, and LinReg software version 1.7 was utilized for analysis of expression. RESULTS: In total, six sequence alterations were identified, which included two stop codons, two frames-shift mutations, one large deletion and one amino acid exchange. FANCA expression was downregulated in patients who had sequence alterations.

  10. Results identified homozygous mutations in FANCA and FANCP/SLX4 genes, both located on chromosome 16, were the affected recessive FA genes in three and one family respectively. Genotyping with short tandem repeat markers and SNP arrays revealed uniparental disomy of the entire mutation-carrying chromosome 16 in all four patients.

  11. Using human and murine cells defective in FANCD2 or FANCA and primary bone marrow cells derived from FANCD2 deficient mice, we show that the FA pathway removes R loops and that many DNA breaks accumulated in FA cells are R loop-dependent.

  12. FANCA safeguards interphase and mitosis during hematopoiesis

  13. The I939S point mutation prevented binding to the FAAP20 subunit of the FA core complex, caused SUMOylation at K921, RNF4-mediated polyubiquitination and degradation.

  14. A frameshifting mutation and a truncating mutation of FANCA are associated with Fanconi anemia.

  15. Proliferation is compromised in FANCA-deficient pluripotent embryonic stem cells.

  16. FANCA-modulated neddylation pathway involved in CXCR5 membrane targeting and cell mobility.

  17. BRCA2 rs10492396 (AG vs. GG: adjusted hazard ratio (adjHR)=1.85, 95% confidence interval (CI)=1.16-2.95, P=0.010), rs206118 (CC vs. TT+TC: adjHR=2.44, 95% CI=1.27-4.67, P=0.007), rs3752447 and FANCA rs62068372

  18. Results suggest that the nonsynonymous single nucleotide polymorphism (rs2239359) in the FANCA gene or other causal variations coexisting with the GGGAGG haplotype may increase risk of premature ovarian failure in Korean women.

  19. Human Fanconi anemia complementation group a protein stimulates the 5' flap endonuclease activity of FEN1.

  20. c.190-256_283 + 1680del2040 dupC mutation in the FANCA gene is a founder mutation in Macedonian Fanconi anemia patients of Gypsy-like ethnic origin.

Mouse (Murine) Fanconi Anemia Group A Protein (FANCA) interaction partners

  1. Using Fanca(-/-) HSCs expressing the separation-of-function mutant p53(515C) transgene, which selectively impairs the p53 function in apoptosis but keeps its cell-cycle checkpoint activities intact, we show that the p53 cell-cycle function is specifically required for the regulation of Fanca(-/-) HSC proliferation.

  2. Fanca(-/-) mice showed a skewed Vkappa gene usage.

  3. FANCA safeguards interphase and mitosis during hematopoiesis

  4. study indicates that Fanca expression during endomitosis is crucial for normal megakaryopoiesis and platelet production.

  5. Data show that Fanconi anemia complementation group A Fanca is required for the induction of transition mutations at A/T residues during somatic hypermutation (SHM) and immunoglobulin (Ig) class switch recombination (CSR).

  6. CD25(+)Foxp3(+) Tregs of Fanca(-/-) or Fancd2(-/-) mice were less efficient in suppressing the production of GVHD-associated inflammatory cytokines.

  7. null mutations in Fanca or Fancg are fully epistatic

  8. genetic diversity in FANCA, FANCC and FANCL does not support an association of these genes with cervical cancer susceptibility in the Swedish population.

  9. The results support a model where both FANCA and FANCC are part of a multi-protein nuclear FA complex with identical function in cellular responses to DNA damage and germ cell survival.

  10. To study the in vivo role of the FA group A gene (Fanca), gene-targeting techniques were used to generate Fanca(tm1Hsc) mice in which Fanca exons 1-6 were replaced by a beta-galactosidase reporter construct.

  11. GnRH induced a rapid, transient increase in Fanca mRNA.

  12. Fanca protein as an integral component in the early step of homologous repair of DNA double-strand brearks thereby minimizing the genomic instability.

  13. Fanca knockout mice presented with microcephalies and a decreased neuronal production in developing cortex and adult brain, demonstrating a critical role for Fanconi pathway in neural stem and progenitor cells during developmental and adult neurogenesis.

Fanconi Anemia Group A Protein (FANCA) Antigen Profile

Protein Summary

The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity\; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group A. Alternative splicing results in multiple transcript variants encoding different isoforms. Mutations in this gene are the most common cause of Fanconi anemia.

Gene names and symbols associated with FANCA

  • Fanconi anemia complementation group A (FANCA) antibody
  • Fanconi anemia, complementation group A (Fanca) antibody
  • AW208693 antibody
  • FA antibody
  • FA-H antibody
  • FA1 antibody
  • FAA antibody
  • FACA antibody
  • FAH antibody
  • FANCH antibody

Protein level used designations for FANCA

Fanconi anemia group A protein , Fanconi anemia, complementation group H , Fanconi anemia, type 1 , Fanconi anemia group A protein homolog

GENE ID SPECIES
2175 Homo sapiens
454393 Pan troglodytes
14087 Mus musculus
415854 Gallus gallus
361435 Rattus norvegicus
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