anti-Heat Shock 22kDa Protein 8 (HSPB8) Antibodies

The protein encoded by HSPB8 belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. Additionally we are shipping Heat Shock 22kDa Protein 8 Proteins (26) and Heat Shock 22kDa Protein 8 Kits (15) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
HSPB8 26353 Q9UJY1
HSPB8 80888 Q9JK92
HSPB8 113906 Q9EPX0
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Top anti-Heat Shock 22kDa Protein 8 Antibodies at antibodies-online.com

Showing 10 out of 228 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Cow Rabbit Un-conjugated WB WB Suggested Anti-HSPB8 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Human Placenta 100 μL Log in to see 2 to 3 Days
$289.00
Details
Cow Rabbit Un-conjugated WB WB Suggested Anti-HSPB8 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: ACHN cell lysate WB Suggested Anti-HSPB8  Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:.2500  Positive Control: ACHN cell lysate  HSPB8 is supported by BioGPS gene expression data to be expressed in ACHN 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Goat Un-conjugated ELISA, IHC, WB ABIN184852 staining (1µg/ml) of human muscle lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. 100 μg Log in to see 6 to 7 Days
$429.62
Details
Human Rabbit Un-conjugated IHC (p), WB Western blot analysis of HSPB8/Hsp22 using anti- HSPB8/Hsp22 antibody .  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each  Lane was loaded with 50ug of sample under reducing conditions.   Lane 1: rat skeletal muscle tissue lysates,   Lane 2: rat heart tissue lysates,   Lane 3: mouse skeletal muscle tissue lysates,   Lane 4: mouse heart tissue lysates,   Lane 5: HELA whole Cell lysates.  After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- HSPB8/Hsp22 antigen affinity purified polyclonal antibody (Catalog # ) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPB8/Hsp22 at approximately 22KD. The expected band size for HSPB8/Hsp22 is at 22KD. IHC analysis of HSPB8/Hsp22 using anti- HSPB8/Hsp22 antibody . HSPB8/Hsp22 was detected in paraffin-embedded section of mouse pancreas tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti- HSPB8/Hsp22 Antibody  overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. 100 μg Log in to see 4 to 6 Days
$280.00
Details
Human Rabbit HRP ICC, IF, IP, IHC, WB Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSP22 Polyclonal Antibody . Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-HSP22 Polyclonal Antibody  at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Nuclear Staining. Western blot analysis of Rat Skeletal muscle lysates showing detection of HSP22 protein using Rabbit Anti-HSP22 Polyclonal Antibody . Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP22 Polyclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. 100 μL Log in to see 3 to 4 Days
$436.80
Details
Human Rabbit FITC ICC, IF, IP, IHC, WB Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSP22 Polyclonal Antibody . Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-HSP22 Polyclonal Antibody  at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Nuclear Staining. Western blot analysis of Rat Skeletal muscle lysates showing detection of HSP22 protein using Rabbit Anti-HSP22 Polyclonal Antibody . Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP22 Polyclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. 100 μL Log in to see 3 to 4 Days
$442.00
Details
Human Rabbit Alkaline Phosphatase (AP) ICC, IF, IP, IHC, WB Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSP22 Polyclonal Antibody . Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-HSP22 Polyclonal Antibody  at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Nuclear Staining. Western blot analysis of Rat Skeletal muscle lysates showing detection of HSP22 protein using Rabbit Anti-HSP22 Polyclonal Antibody . Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP22 Polyclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. 100 μL Log in to see 3 to 4 Days
$444.60
Details
Human Rabbit Biotin ICC, IF, IP, IHC, WB Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSP22 Polyclonal Antibody . Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-HSP22 Polyclonal Antibody  at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Nuclear Staining. Western blot analysis of Rat Skeletal muscle lysates showing detection of HSP22 protein using Rabbit Anti-HSP22 Polyclonal Antibody . Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP22 Polyclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. 100 μL Log in to see 3 to 4 Days
$447.20
Details
Human Rabbit PE ICC, IF, IP, IHC, WB Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSP22 Polyclonal Antibody . Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-HSP22 Polyclonal Antibody  at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Nuclear Staining. Western blot analysis of Rat Skeletal muscle lysates showing detection of HSP22 protein using Rabbit Anti-HSP22 Polyclonal Antibody . Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP22 Polyclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. 100 μL Log in to see 3 to 4 Days
$448.50
Details
Human Rabbit Streptavidin ICC, IF, IP, IHC, WB Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSP22 Polyclonal Antibody . Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-HSP22 Polyclonal Antibody  at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Nuclear Staining. Western blot analysis of Rat Skeletal muscle lysates showing detection of HSP22 protein using Rabbit Anti-HSP22 Polyclonal Antibody . Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP22 Polyclonal Antibody  at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. 100 μL Log in to see 3 to 4 Days
$449.80
Details

Top referenced anti-Heat Shock 22kDa Protein 8 Antibodies

  1. Human Polyclonal HSPB8 Primary Antibody for ELISA, WB - ABIN249692 : Benndorf, Sun, Gilmont, Biederman, Molloy, Goodmurphy, Cheng, Andrews, Welsh: HSP22, a new member of the small heat shock protein superfamily, interacts with mimic of phosphorylated HSP27 ((3D)HSP27). in The Journal of biological chemistry 2001 (PubMed)

  2. Human Polyclonal HSPB8 Primary Antibody for ICC, IF - ABIN4320602 : Stadler, Rexhepaj, Singan, Murphy, Pepperkok, Uhlén, Simpson, Lundberg: Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. in Nature methods 2013 (PubMed)

More Antibodies against Heat Shock 22kDa Protein 8 Interaction Partners

Human Heat Shock 22kDa Protein 8 (HSPB8) interaction partners

  1. We identified impaired RNA metabolism, secondary to TDP-43 (show TARDBP Antibodies) loss of function, as a possible pathological mechanism of HSPB8 toxicity, leading to muscle and nerve degeneration

  2. Data indicated that the HSPB8 expression level was strongly positively correlated with MAPK (show MAPK1 Antibodies) signaling pathway and CREB (show CREB1 Antibodies) pathway and worse prognosis. The methylation level of its DNA was negatively associated with its expression and positively associated with overall survival. These results suggest that HSPB8 could promote the proliferation and inhibit the apoptosis of GC cells by activating ERKCREB signaling.

  3. HSPB2 (show HSPB2 Antibodies) competes with HSPB8 for binding to BAG3 (show BAG3 Antibodies). In contrast, HSPB3 (show HSPB3 Antibodies) negatively regulates HSPB2 (show HSPB2 Antibodies) association with BAG3 (show BAG3 Antibodies).

  4. Silencing of HSPB8 markedly decreased the mitotic levels of BAG3 in HeLa cells, supporting its crucial role in BAG3 mitotic functions. The results support a role for the HSPB8-BAG3 chaperone complex in quality control of actin-based structure dynamics that are put under high tension, notably during cell cytokinesis.

  5. HSPB8 is involved in regulating the cell cycle and cell migration in MCF-7 cells.

  6. suggest that HSPB8 may act as an intracellular factor against hepatitis C virus replication and that DNAJC5B has the same function, with more relevant results for genotype 3

  7. It has been demonstrated that HSPB8-BAG3 (show BAG3 Antibodies)-HSP70 (show HSP70 Antibodies) ensures the functionality of stress granules and restores proteostasis by targeting defective ribosomal products for degradation.

  8. We report that overexpression of HSPB8 in immortalized motor neurones decreased the accumulation of TDP-25 and TDP-35 and that protection against mislocalized/truncated TDP-43 (show TARDBP Antibodies) was observed for HSPB8 in Drosophila melanogaster Overexpression of HSP67Bc, the functional ortholog of human HSPB8, suppressed the eye degeneration caused by the cytoplasmic accumulation of a TDP-43 (show TARDBP Antibodies) variant

  9. HSPB8 counteracts accumulation of aberrantly localized misfolded forms of TDP-43 (show TARDBP Antibodies) and its 25 KDa fragment involved in most sporadic cases of Amyotrophic Lateral Sclerosis and of Fronto Lateral Temporal Dementia.

  10. expands the understanding of disease mechanisms, tissue involvement, and phenotypic outcome of HSPB8 mutations

Cow (Bovine) Heat Shock 22kDa Protein 8 (HSPB8) interaction partners

  1. GA genotype of SNP g.507G>A of HSPB8 gene has a probable role in heat tolerance in Sahiwal cattle.

Mouse (Murine) Heat Shock 22kDa Protein 8 (HSPB8) interaction partners

  1. HSP22 functions as a negative regulator in the TGF-beta (show TGFB1 Antibodies)-stimulated migration of osteoblasts.

  2. Results revealed that the apoptosis-suppressing effect of heat shock protein B8 was mediated by the PI3K/Akt (show AKT1 Antibodies) pathway in an in vitro model of ischemia.

  3. Increased Hsp20 (show HSPB6 Antibodies) expression protects against OGDR-induced Golgi fragmentation and apoptosis, likely through interaction with Bax (show BAX Antibodies) and subsequent amelioration of the OGDR-induced elevation in p115 (show ARHGAP4 Antibodies) cleavage via the Fas (show FAS Antibodies)/FasL (show FASL Antibodies) signaling pathway.

  4. Translocation of both Hsp22 and iNOS to the mitochondria is necessary for Hsp22-mediated stimulation of oxidative phosphorylation

  5. analysis of the phenotype of cardiomyopathy in cardiac-specific heat shock protein B8 K141N transgenic mouse

  6. The results of this study strongly suggested that Hspb8 and its alpha-crystallin domain might act as pleiotropic prosurvival factor in the adult hippocampus.

  7. Hspb8 mRNA is constitutively expressed in specific brain structures across ontogeny; eventually these structures could be affected by the malfunction or deregulation of the Hspb8 molecule.

  8. Hsp22 represents a previously undescribed activator of both nuclear and mitochondrial functions of STAT3 (show STAT3 Antibodies), and its deletion in the context of pressure overload in vivo accelerates the transition into heart failure and increases mortality.

  9. HspB8 increases misfolded SOD1 (show SOD1 Antibodies) clearance via autophagy.

  10. Findings show that despite the ubiquitous presence of HSPB8, only motor neurons appear to be affected by the K141N and K141E mutations.

Heat Shock 22kDa Protein 8 (HSPB8) Antigen Profile

Protein Summary

The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.

Gene names and symbols associated with HSPB8

  • heat shock protein family B (small) member 8 (HSPB8) antibody
  • heat shock protein family B (small) member 8 L homeolog (hspb8.L) antibody
  • heat shock protein 8 (hspa8) antibody
  • heat shock protein b8 (hspb8) antibody
  • heat shock protein 8 (Hspb8) antibody
  • heat shock protein family B (small) member 8 (Hspb8) antibody
  • AU018630 antibody
  • AW413033 antibody
  • CMT2L antibody
  • Cryac antibody
  • D5Ucla4 antibody
  • DHMN2 antibody
  • E2IG1 antibody
  • fc09c11 antibody
  • H11 antibody
  • H11K antibody
  • HMN2 antibody
  • HMN2A antibody
  • hsc70 antibody
  • HSP20-like antibody
  • Hsp22 antibody
  • MGC64408 antibody
  • wu:fb01g06 antibody
  • wu:fc04b04 antibody
  • wu:fc09c11 antibody
  • wu:fi48b06 antibody
  • zgc:64202 antibody

Protein level used designations for HSPB8

E2-induced gene 1 protein , alpha-crystallin C chain , heat shock 27kDa protein 8 , heat shock protein beta-8 , protein kinase H11 , small stress protein-like protein HSP22 , heat shock 22kDa protein 8 , H11 kinase , heat shock 70 kDa protein 8 , heat shock 70kDa protein 8 , heat shock cognate 71 kDa protein , heat shock cognate 71-kd protein , crystallin, alpha C , heat shock protein 20-like protein , heat shock protein 8

GENE ID SPECIES
26353 Homo sapiens
379472 Xenopus laevis
403553 Canis lupus familiaris
416988 Gallus gallus
452295 Pan troglodytes
539524 Bos taurus
574341 Macaca mulatta
100174469 Pongo abelii
100349029 Oryctolagus cuniculus
573376 Danio rerio
791580 Danio rerio
80888 Mus musculus
113906 Rattus norvegicus
100734199 Cavia porcellus
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