anti-Heat Shock Protein, alpha-Crystallin-Related, B6 (HSPB6) Antibodies

This locus encodes a heat shock protein. Additionally we are shipping Heat Shock Protein, alpha-Crystallin-Related, B6 Kits (37) and Heat Shock Protein, alpha-Crystallin-Related, B6 Proteins (14) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
HSPB6 126393 O14558
HSPB6 192245 P97541
HSPB6 243912 Q5EBG6
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Top anti-Heat Shock Protein, alpha-Crystallin-Related, B6 Antibodies at antibodies-online.com

Showing 10 out of 138 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Delivery Price Details
Human Rabbit Un-conjugated ELISA, ICC, IF, IHC, WB ABIN6267220 staining HeLa cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody. ABIN6267220 at 1/100 staining mouse gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary. 100 μL 11 to 12 Days
$390.77
Details
Cow Rabbit Un-conjugated WB WB Suggested Anti-HSPB6 Antibody Titration:  1.25ug/ml  Positive Control:  HepG2 cell lysate 100 μL 2 to 3 Days
$249.00
Details
Cow Rabbit Un-conjugated IC, IF, IHC, WB Immunofluorescent analysis of HSP20 (pS16) staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary Immunohistochemical analysis of HSP20 (pS16) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incub 200 μL 13 to 14 Days
$575.00
Details
Human Rabbit Un-conjugated WB Western blot analysis of Hsp20 using anti-Hsp20 antibody .  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each  Lane was loaded with 50ug of sample under reducing conditions.   Lane 1: rat cardiac muscle tissue lysate, Lane 2: rat skeletal muscle tissue lysate, Lane 3: rat testis tissue lysate, Lane 4: mouse testis tissue lysate, Lane 5: human A431 whole Cell lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp20 antigen affinity purified polyclonal antibody (Catalog # ) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsp20 at approximately 17KD. The expected band size for Hsp20 is at 17KD. 100 μg 4 to 6 Days
$240.00
Details
Human Rabbit Un-conjugated IHC, ELISA Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using HSP20 (Ab-16) Antibody. The picture on the right is treated with the synthesized peptide. 100 μg 2 to 3 Days
$302.50
Details
Human Rabbit Un-conjugated IF, IHC, ELISA Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HSP20 (Phospho-Ser16) Antibody. The picture on the right is treated with the synthesized peptide. Immunofluorescence analysis of HeLa cells, using HSP20 (Phospho-Ser16) Antibody. The picture on the right is treated with the synthesized peptide. 100 μg 2 to 3 Days
$302.50
Details
Human Rabbit Un-conjugated ELISA, IHC, IHC (p) Anti-HSPB6 antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody  ABIN959333 concentration 10 ug/ml. 50 μL 11 to 14 Days
$484.00
Details
Cow Rabbit Un-conjugated WB 100 μL 11 to 14 Days
$507.83
Details
Human Mouse Un-conjugated ELISA, IF, IHC, IHC (p), WB Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE) Anti-HSPB6 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. 0.25 mg 11 to 14 Days
$550.00
Details
Cow Rabbit Un-conjugated IHC, IHC (p), IP, WB Human Skeletal Muscle (formalin-fixed, paraffin-embedded) stained with HSPB6 antibody ABIN364293 at 2.5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody ABIN481713, alkaline phosphatase-streptavidin and chromogen. Human Prostate (formalin-fixed, paraffin-embedded) stained with HSPB6 antibody ABIN364293 at 2.5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody ABIN481713, alkaline phosphatase-streptavidin and chromogen. 50 μg 11 to 14 Days
$639.83
Details

Top referenced anti-Heat Shock Protein, alpha-Crystallin-Related, B6 Antibodies

  1. Cow (Bovine) Polyclonal HSPB6 Primary Antibody for WB - ABIN266034 : Ju, Kwag, Park, Jung, Jeong, Jeong, Lee, Choi, Kang, Hah, Hong: Decreased expression of heat shock protein 20 in colorectal cancer and its implication in tumorigenesis. in Journal of cellular biochemistry 2014 (PubMed)

More Antibodies against Heat Shock Protein, alpha-Crystallin-Related, B6 Interaction Partners

Human Heat Shock Protein, alpha-Crystallin-Related, B6 (HSPB6) interaction partners

  1. these findings reveal a new regulatory mechanism of HSPB6 in cell survival through its interaction with BECN1.

  2. Structural basis for the interaction of a human HSPB6 protein with the 14-3-3 universal signaling regulator has been reported.

  3. Data suggest that HSPB6 forms hetero-oligomers with HSPB1 under the following rules: (1) highly conserved motif RLFDQXFG is necessary for subunit exchange among oligomers, (2) a site about 20 residues downstream of this motif determines size of resultant hetero-oligomers, and (3) a region in the N-terminal domain that is unique to HSPB6 dictates preferential formation of heterodimers. (HSP = heat shock protein)

  4. findings strongly suggest that phosphorylated HSP20 inhibits TGF-alpha-induced HCC cell migration and invasion via suppression of the JNK signaling pathway

  5. multiple sclerosis lesions revealed exclusive induction of HSPB6 in astrocytes, as confirmed by co-localization of HSPB6 with GFAP.

  6. N-terminal mutations increase stability of large HspB1 homooligomers, prevent their phosphorylation-dependent dissociation, modulate their interaction with HspB6 and decrease their chaperoning capacity, preventing normal functioning of HspB1.

  7. Findings strongly suggest that HSP20 directly associates with Bax and stimulates caspase cascade in human hepatocellular carcinoma cells.

  8. Data suggest that heat shock protein 20 (HSP20) may have value as a prognostic tumor marker and its overexpression might be a novel strategy for colorectal cancer (CRC) therapy.

  9. peptides in heat-shock protein Hsp20 (G71HFSVLLDVKHFSPEEIAVK91) and Hsp27 (D93RWRVSLDVNHFAPDELTVK113) with sequence homology to alpha-crystallin also have robust chaperone and anti-apoptotic activities.

  10. These findings strongly suggest that HSP20 might decrease the IKK-alpha protein level and that it down-regulates the TNF-alpha-stimulated intracellular signaling in HCC, thus resulting in the suppression of HCC progression.

  11. HSP20 may play a protective role against the progression of ovarian cancer.

  12. HSP20 directly associates with PI3K subunits and suppresses its activity in hepatocellular carcinoma, resulting in the inhibition of the AKT pathway, and subsequently decreasing the growth of hepatocellular carcinoma.

  13. 14-3-3zeta and possibly other 14-3-3 isoforms may have additional functional roles conducted by the monomeric state

  14. the cytosolic protein AKAP-Lbc (AKAP13) as the anchoring protein responsible for directing PKA phosphorylation of Hsp20 on Ser(16)

  15. Hsp20 serves as a novel cardiokine in regulating myocardial angiogenesis through activation of the VEGFR signaling cascade.

  16. cofilins 1 and 2 only weakly interact with 14-3-3 and therefore cannot directly compete with phosphorylated small heat shock protein HspB6 for its binding to 14-3-3

  17. Properties of the monomeric form of 14-3-3zeta protein and its interaction with tau and HspB6. This interaction requires phosphorylation of tau protein and HspB6.

  18. A potential molecular mechanism by which Hsp20 acetylation can affect myometrial activity by liberating cofilin

  19. Overexpression of HSPB1, as well as HSPB6, HSPB7 and HSPB8 independently protect against tachycardia remodeling by attenuation of the RhoA GTPase pathway at different levels.

  20. Nevertheless, in solution, both alpha-crystallin domain proteins form stable dimers via the symmetric antiparallel interaction of beta7 strands.

Mouse (Murine) Heat Shock Protein, alpha-Crystallin-Related, B6 (HSPB6) interaction partners

  1. Our study was designed to assess the efficacy of small heat shock proteins B1 (Hsp27) and B6 (Hsp20) as an adjuvant accompanied by HPV16 E7 and hPP10-E7 antigens in tumor mouse model. A major key for successful DNA and protein transfer into cells is the development of delivery systems with high efficiency and low cytotoxicity.

  2. Increased expression of Hsp20 in neuroblastoma cells protected against ischemia-reperfusion injury.

  3. potential protein targets for the loss of PostC may include F(1)-ATPase gamma, Echs1 and Hsp20 that could regulate cellular ATP consumption/production and defense response to ischaemic stress

  4. the activity of type 1 protein phosphatase (PP1), a known regulator of PLN signaling, was significantly reduced by Hsp20 overexpression, suggesting that the Hsp20 stimulatory effects are partially mediated through the PP1-PLN axis.

  5. identification and characterization of a cardiac isoform of p20 associated with beta-adrenergic signaling in adult cardiomyocytes.

  6. Recombinant mouse Hsp20 translocates to and interacts with actin cytoskeleton in response to isoproterenol stimulation and prevents ss-agonist-induced apoptosis in adult rat cardiomyocytes.

  7. miR-320 is involved in the regulation of I/R-induced cardiac injury and dysfunction via antithetical regulation of Hsp20

  8. Hsp20 expression prevents endotoxin-induced myocardial dysfunction and apoptosis via inhibition of NF-kappaB activation

  9. Blockade of Hsp20 phosphorylation exacerbates cardiac ischemia/reperfusion injury by suppressed autophagy and increased cell death.

Xenopus laevis Heat Shock Protein, alpha-Crystallin-Related, B6 (HSPB6) interaction partners

  1. HSPB6 cDNA encodes a 168 aa protein that contains an alpha-crystallin domain, a polar C-terminal extension and some possible phosphorylation sites. HSPB6 shares 94% identity with a X. tropicalis HSPB6, 65% with turtle, 59% with humans, 49% with zebrafish and only 50% and 43% with X. laevis HSPB1 and HSP30C, respectively.

Cow (Bovine) Heat Shock Protein, alpha-Crystallin-Related, B6 (HSPB6) interaction partners

  1. HSP20 is associated with both actin and alpha-actinin; activation of cyclic nucleotide-dependent signaling pathways leads to increases in the phosphorylation of HSP20 and a decrease in the association of HSP20 with alpha-actinin.

Zebrafish Heat Shock Protein, alpha-Crystallin-Related, B6 (HSPB6) interaction partners

  1. HSPB6 is expressed during development.

Heat Shock Protein, alpha-Crystallin-Related, B6 (HSPB6) Antigen Profile

Protein Summary

This locus encodes a heat shock protein. The encoded protein likely plays a role in smooth muscle relaxation.

Gene names and symbols associated with HSPB6

  • heat shock protein family B (small) member 6 (HSPB6) antibody
  • heat shock protein family B (small) member 6 (Hspb6) antibody
  • heat shock protein, alpha-crystallin-related, B6 (Hspb6) antibody
  • heat shock protein family B (small) member 6 L homeolog (hspb6.L) antibody
  • heat shock protein, alpha-crystallin-related, b6 (hspb6) antibody
  • AA387366 antibody
  • Gm479 antibody
  • HSP20 antibody
  • p20 antibody

Protein level used designations for HSPB6

heat shock 20 kDa-like protein p20 , heat shock protein beta-6 , heat shock 20-kDa protein , cardiac heat shock protein p20 , cardiac p20 , hspb13 , small heat shock protein HSPB6 , heat shock protein, alpha-crystallin-related, B6

GENE ID SPECIES
126393 Homo sapiens
192245 Rattus norvegicus
243912 Mus musculus
446313 Xenopus laevis
534551 Bos taurus
792610 Danio rerio
100347944 Oryctolagus cuniculus
484574 Canis lupus familiaris
100724640 Cavia porcellus
100146605 Equus caballus
101104335 Ovis aries
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