anti-Microtubule Associated serine/threonine Kinase-Like (MASTL) Antibodies

MASTL encodes a microtubule-associated serine/threonine kinase. Additionally we are shipping MASTL Kits (8) and MASTL Proteins (5) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
MASTL 84930 Q96GX5
MASTL 67121 Q8C0P0
MASTL 307169  
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Top anti-MASTL Antibodies at antibodies-online.com

Showing 10 out of 100 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Delivery Price Details
Cow Rabbit Un-conjugated WB 100 μL 2 to 3 Days
$289.00
Details
Human Rabbit Un-conjugated ELISA, WB Western blot analysis of extracts from HepG2 cells, using MASTL Antibody. The lane on the right is treated with the synthesized peptide. 100 μg 2 to 3 Days
$302.50
Details
Human Rabbit Un-conjugated ELISA, IHC, WB 100 μL Available
$363.46
Details
Human Mouse Un-conjugated IF, WB MASTL  western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the MASTL antibody detected the MASTL protein (arrow). Confocal immunofluorescent analysis of MASTL Antibody  with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue). 400 μL 10 to 11 Days
$385.00
Details
Mouse Rabbit Un-conjugated WB Mouse Mastl Antibody (Center) (ABIN658210) western blot analysis in mouse liver tissue lysates (35 µg/lane). This demonstrates the Mastl antibody detected the Mastl protein (arrow). 400 μL 10 to 11 Days
$385.00
Details
Human Rabbit Un-conjugated ELISA, ICC, IF, WB Western blot analysis of extracts from HeLa cells, using MASTL antibody. ABIN6275848 staining  HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25¡ãC. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37¡ãC. An  Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibod 100 μL 11 to 12 Days
$390.77
Details
Human Rabbit Un-conjugated ELISA, ICC, IF, WB ABIN6274474 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Cat.# S0006), diluted at 1/600, was used as secondary antibody. Western blot analysis of extracts from HepG2 cells, using MASTL antibody.The lane on the left is treated with the antigen-specific peptide. 100 μL 11 to 12 Days
$390.77
Details
Cow Rabbit Un-conjugated WB 100 μL 11 to 14 Days
$551.83
Details
Human Rabbit Un-conjugated FACS, IHC (p), WB Western blot analysis of MASTL Antibody (ABIN652294) in HepG2, MDA-MB231, 293, Hela cell line lysates (35 µg/lane). MASTL (arrow) was detected using the purified polyclonal antibody. Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MASTL Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. 400 μL 10 to 11 Days
$385.00
Details
Mouse Rabbit Un-conjugated WB Western blot analysis in mouse liver tissue lysates (35ug/lane). 400 μL 2 to 3 Days
$515.63
Details

Top referenced anti-MASTL Antibodies

  1. Human Polyclonal MASTL Primary Antibody for IHC, IHC (p) - ABIN439669 : Della Monica, Visconti, Cervone, Serpico, Grieco: Fcp1 phosphatase controls Greatwall kinase to promote PP2A-B55 activation and mitotic progression. in eLife 2016 (PubMed)

  2. Human Polyclonal MASTL Primary Antibody for ICC, IF - ABIN4332787 : Hégarat, Vesely, Vinod, Ocasio, Peter, Gannon, Oliver, Novák, Hochegger: PP2A/B55 and Fcp1 regulate Greatwall and Ensa dephosphorylation during mitotic exit. in PLoS genetics 2014 (PubMed)

More Antibodies against MASTL Interaction Partners

Zebrafish Microtubule Associated serine/threonine Kinase-Like (MASTL) interaction partners

  1. transient knockdown of MASTL correlates with a decrease in the expression of c-mpl and GpIIb, and reduction of circulating thrombocytes

Human Microtubule Associated serine/threonine Kinase-Like (MASTL) interaction partners

  1. Data show that MASTL expression increases in colon cancer (CC) across all cancer stages. Also, increased levels of MASTL associated with high-risk disease and poor prognosis. Further, its silencing induced cell cycle arrest and apoptosis in vitro and inhibited xenograft-tumor growth. Functional analysis revealed that MASTL expression facilitates CC progression and chemoresistance by promoting the beta-catenin/Wnt signa...

  2. MASTL overexpression contributes to chromosome instability and metastasis, thereby decreasing breast cancer patient survival.

  3. E2F8 can shorten cisplatin induced G2/M arrest by promoting MASTL mediated mitotic progression in ER+ breast cancer cells, conferring drug resistance.

  4. Using mathematical modelling, this paper confirms that deactivation of MASTL is essential for mitotic exit.

  5. these results established that precise control of MASTL is essential to couple DNA damage to mitosis through the rate of mitotic entry and APC/C activation.

  6. Thus, GWL is a human oncoprotein that promotes the hyperactivation of AKT via the degradation of its phosphatase, PHLPP, in human malignancies.

  7. Thus, Fcp1 coordinates Cdk1 and Gwl inactivation to derepress PP2A-B55, generating a dephosphorylation switch that drives mitosis progression.

  8. Boolean modeling identifies Greatwall/MASTL as an important regulator in the AURKA network of neuroblastoma.

  9. Data show that siRNA knockdown of Forkhead box M1 (FOXM1) or microtubule-associated serine/threonine kinase-like (MASTL) induces radiosensitivity in non-small cell lung cancer (NSCLC).

  10. Mastl upregulation is involved in cancer progression and tumor recurrence after initial cancer therapy

  11. data demonstrate that GWL acts in a pathway with PP2A which is essential for prophase I exit and metaphase I microtubule assembly in mouse oocytes.

  12. Taken together our results suggest a hierarchy of phosphatases coordinating Greatwall, Ensa/ARPP19 and Cdk substrate dephosphorylation during mitotic exit.

  13. Studies indicate that mutations in three different genes within the THC2 locus have been associated with congenital thrombocytopenia, including a mutation in MASTL.

  14. results identify Gwl as a member of the AGC family of kinases that appears to be regulated by unique mechanisms and that differs from the other members of this family

  15. MASTL enhances cyclin B1-Cdk1-dependent mitotic phosphorylation events, directing mitotic entry, anaphase and cytokinesis in human cells.

  16. A paper that narrows the identity of the gene for autosomal dominant thrombocytopenia (THC2) to FLJ14813. The mutation is present in all affected people across three generations while is absent in unaffected family members & 94 random blood donors.

Mouse (Murine) Microtubule Associated serine/threonine Kinase-Like (MASTL) interaction partners

  1. Findings reveal the role of RSK in mouse oocytes, showing that the RSK-MASTL pathway allows mammalian-specific prolonged meiotic exit and ensures the faithful conversion from sperm to paternal pronuclei.

  2. using in vitro dephosphorylation assays, we demonstrate that Mastl promotes persistent MPS1 phosphorylation by inhibiting PP2A/B55-mediated MPS1 dephosphorylation rather than affecting Cdk1 kinase activity. Our findings establish a key regulatory function of the Greatwall kinase/Mastl - PP2A/B55 pathway in preventing premature SAC silencing

  3. Mastl is required for the timely activation of anaphase-promoting complex/cyclosome to allow meiosis I exit and for the rapid rise of Cdk1 activity.

  4. Data show that Mastl (Greatwall)-null cells display mitotic collapse after nuclear envelope breakdown (NEB) characterized by defective chromosome condensation and prometaphase arrest.

Xenopus laevis Microtubule Associated serine/threonine Kinase-Like (MASTL) interaction partners

  1. Data suggest Greatwall kinase (Gwl) associates with protein phosphatase 1 (PP1), particularly PP1gamma subunit, which mediates dephosphorylation of Gwl Ser-883; consistent with mitotic activation of Gwl, its association with PP1 is disrupted in mitotic cells; subunits PPP1R3B and PPP1R13L associate with Gwl; thus, PPP1R3B appears to act as cell cycle regulator in oocytes that functions by governing Gwl dephosphorylation.

  2. Full dephosphorylation of Gwl results in complete inactivation of Arpp19 and ENSA, and dephosphorylation of mitotic substrates. this feed-back loop irreversibly induces mitotic exit.

  3. study provides evidence that PP1 targets the auto-phosphorylation site of Gwl, resulting in efficient Gwl inactivation; this step is necessary to facilitate subsequent complete dephosphorylation of Gwl by PP2A-B55

  4. we showed that the Gwl nuclear localization is indispensable for the biochemical function of Gwl in promoting mitotic entry.

  5. PP2A-B55delta, Greatwall and ARPP19 are not only required for entry into meiotic divisions, but are also pivotal effectors within the Cdk1 auto-regulatory loop responsible for its independence with respect to the PKA-negative control.

  6. Greatwall kinase and cyclin B-Cdk1 are both critical constituents of M-phase-promoting factor.

  7. inhibition of PP2A-B55delta results from Ensa, that is phosphorylated in mitosis by the protein kinase Greatwall; this converts Ensa into specific inhibitor of PP2A-B55delta; this pathway represents a previously unknown element in mitosis control

  8. 3 phosphorylation sites (phosphosites) critical to Gwl activation (pT193, pT206, and pS883 in Xenopus laevis) located in evolutionarily conserved domains that differentiate Gwl from related kinases

  9. Coordinated interplays between Plx1 and Gwl are required for reactivation of these kinases from the G(2)/M DNA damage checkpoint and efficient checkpoint recovery.

  10. mitotic entry and maintenance is not only mediated by the activation of cyclin B-Cdc2 but also by the regulation of PP2A by GW

  11. [review] The kinase Greatwall phosphorylates small protein ARPP-19 and converts it into a potent antimitotic PP2A inhibitor.

MASTL Antigen Profile

Protein Summary

This gene encodes a microtubule-associated serine/threonine kinase. Mutations at this locus have been associated with autosomal dominant thrombocytopenia, also known as thrombocytopenia-2. Alternatively spliced transcript variants have been described for this locus.

Gene names and symbols associated with MASTL

  • microtubule associated serine/threonine kinase-like (mastl) antibody
  • microtubule associated serine/threonine kinase like (MASTL) antibody
  • serine/threonine-protein kinase greatwall (LOC100541707) antibody
  • microtubule associated serine/threonine kinase-like (Mastl) antibody
  • microtubule associated serine/threonine kinase like L homeolog (mastl.L) antibody
  • 2700091H24Rik antibody
  • C88295 antibody
  • greatwall antibody
  • GW antibody
  • GWL antibody
  • hGWL antibody
  • im:7149025 antibody
  • MAST-L antibody
  • MASTL antibody
  • RP11-85G18.2 antibody
  • thc2 antibody
  • wu:fa02a12 antibody
  • zgc:100990 antibody

Protein level used designations for MASTL

microtubule-associated serine/threonine-protein kinase-like , serine/threonine-protein kinase greatwall , microtubule associated serine/threonine kinase-like , greatwall protein kinase , Serine/threonine-protein kinase greatwall , Microtubule-associated serine/threonine-protein kinase-like

GENE ID SPECIES
445215 Danio rerio
450367 Pan troglodytes
708134 Macaca mulatta
100441835 Pongo abelii
100514867 Sus scrofa
100541707 Meleagris gallopavo
100602092 Nomascus leucogenys
84930 Homo sapiens
67121 Mus musculus
307169 Rattus norvegicus
540206 Bos taurus
420487 Gallus gallus
607216 Canis lupus familiaris
100464443 Ailuropoda melanoleuca
414585 Xenopus laevis
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