anti-Midline 1 (MID1) Antibodies

The protein encoded by MID1 is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins.

list all antibodies Gene Name GeneID UniProt
Anti-Human MID1 MID1 4281 O15344
Anti-Mouse MID1 MID1 17318 O70583
Anti-Rat MID1 MID1 54252 P82458
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Top anti-MID1 Antibodies at antibodies-online.com

Showing 10 out of 66 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Cow Rabbit Un-conjugated WB WB Suggested Anti-MID1 Antibody Titration:  2.5ug/ml  ELISA Titer:  1:312500  Positive Control:  Jurkat cell lysate WB Suggested Anti-MID1 <br /> Antibody Titration: 2.5 µg/mL ELISA Titer: 1:.12500 <br /> Positive Control: Jurkat cell lysate 100 μL Log in to see 2 to 3 Days
$229.00
Details
Dog Rabbit Un-conjugated IC, IF, IHC, WB Immunofluorescent analysis of MID1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody i Immunohistochemical analysis of MID1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated wit 200 μL Log in to see 13 to 14 Days
$487.50
Details
Human Rabbit Un-conjugated WB MID1 Antibody (C-term) (ABIN657388) western blot analysis in MDA-MB435 cell line lysates (35 µg/lane). This demonstrates the MID1 antibody detected the MID1 protein (arrow). 400 μL Log in to see 10 to 11 Days
$324.50
Details
Cow Rabbit Un-conjugated WB 100 μL Log in to see 2 to 3 Days
$289.00
Details
Bat Rabbit Un-conjugated WB 100 μg Log in to see 7 to 9 Days
$493.17
Details
Human Rabbit Un-conjugated ELISA, IF, IHC, WB Western Blot (WB) analysis of specific cells using Midline-1 Polyclonal Antibody. 100 μL Log in to see 16 Days
$181.73
Details
Human Rabbit Un-conjugated ELISA, IF, IHC, IHC (p), WB 100 μL Log in to see 7 to 9 Days
$493.17
Details
Human Rabbit Un-conjugated WB Western Blot: MID1 Antibody [NBP1-26612] - Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T; 50 ug) cells. Antibody used at 1 ug/ml. 100 μL Log in to see 7 to 9 Days
$556.31
Details
Mouse Rabbit Un-conjugated ICC, IHC, WB Figure. Western Blot; Sample: Recombinant protein. DAB staining on IHC-P; Samples: Mouse Skeletal muscle Tissue 100 μg Log in to see 13 to 16 Days
$360.00
Details
Dog Rabbit Un-conjugated ELISA, WB 100 μg Log in to see 2 to 3 Days
$446.88
Details

Top referenced anti-MID1 Antibodies

  1. Human Polyclonal MID1 Primary Antibody for WB - ABIN439762 : Bell, Malyukova, Holien, Koach, Parker, Kavallaris, Marshall, Cheung: TRIM16 acts as an E3 ubiquitin ligase and can heterodimerize with other TRIM family members. in PLoS ONE 2012 (PubMed)

  2. Human Monoclonal MID1 Primary Antibody for ELISA, WB - ABIN517934 : Liu, Knutzen, Krauss, Schweiger, Chiang: Control of mTORC1 signaling by the Opitz syndrome protein MID1. in Proceedings of the National Academy of Sciences of the United States of America 2011 (PubMed)

More Antibodies against MID1 Interaction Partners

Human Midline 1 (MID1) interaction partners

  1. Data show that protein phosphatase-2A (PP2A (show PPP2R4 Antibodies)) was upregulated in lung adenocarcinoma cell lines that were transfected with midline 1 E3 ubiquitin-protein ligase (show UBE2K Antibodies) (MID1)-siRNA, suggesting MID1 negatively regulates PP2A (show PPP2R4 Antibodies) in lung adenocarcinoma.

  2. identified four miRNAs, miR (show MLXIP Antibodies)-19, miR (show MLXIP Antibodies)-340, miR (show MLXIP Antibodies)-374 and miR (show MLXIP Antibodies)-542 that bind to the 3'-UTR (show UTS2R Antibodies) of the MID1 mRNA. These miRNAs not only regulate MID1 expression but also mTOR (show FRAP1 Antibodies) signaling and translation of disease associated mRNAs and could therefore serve as potential drugs for future therapy development

  3. Our data reveal a novel role for MID1 and for atypical ubiquitination in balancing BRAF35 (show HMG20B Antibodies) presence, and likely its activity, within nuclear and cytoplasmic compartments

  4. P151L MID1 mutation is associated with X-linked Opitz Syndrome.

  5. the coiled-coil and COS domain (CC-COS) bind to microtubules, demonstrating for the first time that MID1 can directly associate with the microtubules

  6. Osx is upregulated in patients with Ossification of the posterior longitudinal ligament.

  7. A130T/V mutations within the MID1 zinc-binding Bbox1 (show BBOX1 Antibodies) domain affects protein folding.

  8. MID1 catalyzes the ubiquitination of protein phosphatase 2A and mutations within its Bbox1 (show BBOX1 Antibodies) domain disrupt polyubiquitination of alpha4 but not of PP2Ac (show PPP2CA Antibodies) in X-linked Opitz syndrome.

  9. TRAIL regulates MID1 and TSLP (show TSLP Antibodies), inflammation, fibrosis, smooth muscle hypertrophy, and expression of inflammatory effector chemokines and cytokines in experimental eosinophilic esophagitis.

  10. These studies provide insight into the mechanism by which mutations observed in X-linked Opitz G syndrome affect the structure and function of the MID1 Bbox1 (show BBOX1 Antibodies) domain

Mouse (Murine) Midline 1 (MID1) interaction partners

  1. we show that MID1 controls exocytosis of lytic granules and cytotoxicity in murine cytotoxic lymphocytes

  2. X-linked microtubule-associated protein (show SPAG5 Antibodies), Mid1, regulates axon development.

  3. MID1 inhibition also limited rhinovirus-induced exacerbation of allergic airway disease

  4. Thus, lack of Mid1 causes a misspecification of the midbrain/cerebellar boundary that results in an abnormal development of the most anterior cerebellar lobes.

  5. Analysis of Mid1, Hccs (show HCCS Antibodies), Arhgap6 (show ARHGAP6 Antibodies), and Msl3l1 (show MSL3 Antibodies) in X-linked polydactyly (Xpl) and Patchy-fur (Paf (show PAF Antibodies)) mutant mice

Xenopus laevis Midline 1 (MID1) interaction partners

  1. SHH (show SHH Antibodies)-dependent E-ligase Midline1 regulates ubiquitin-mediated proteasomal degradation of Pax6 (show PAX6 Antibodies) during visual system development.

MID1 Antigen Profile

Protein Summary

The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Multiple different transcript variants are generated by alternate splicing\; however, the full-length nature of some of the variants has not been determined.

Gene names and symbols associated with anti-Midline 1 (MID1) Antibodies

  • midline 1 (MID1) antibody
  • midline 1 (Mid1) antibody
  • midline 1 (mid1) antibody
  • midline 1 L homeolog (mid1.L) antibody
  • 61B3-R antibody
  • bbbg1 antibody
  • DXHXS1141 antibody
  • fxy antibody
  • gbbb1 antibody
  • midin antibody
  • Midline1 antibody
  • ogs1 antibody
  • OS antibody
  • osx antibody
  • rnf59 antibody
  • trim18 antibody
  • xprf antibody
  • znfxy antibody

Protein level used designations for anti-Midline 1 (MID1) Antibodies

RING finger protein 59 , midline 1 RING finger protein , midline-1 , putative transcription factor XPRF , tripartite motif protein TRIM18 , tripartite motif-containing protein 18 , zinc finger on X and Y, mouse, homolog of , midin , Finger on X and Y (in rat only on X) , E3 ubiquitin-protein ligase Midline-1 , midline 1 (Opitz/BBB syndrome)

GENE ID SPECIES
4281 Homo sapiens
17318 Mus musculus
54252 Rattus norvegicus
373920 Gallus gallus
100330952 Danio rerio
100463496 Xenopus laevis
491737 Canis lupus familiaris
534604 Bos taurus
100524415 Sus scrofa
100716416 Cavia porcellus
100049999 Equus caballus
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