Neurofilament, Heavy Polypeptide Proteins (NEFH)

Mouse (Murine) Neurofilament, Heavy Polypeptide (NEFH) interaction partners

1. These findings indicate that NEFH is expressed in podocytes during the disease course and that it prevents the reduction in synaptopodin expression and detachment of podocytes.

2. The abundance of dural afferent neurons expressing NF200 in adult mice.

3. Data indicate the presence of a HuD - BDNF - NF-H pathway activated as a regenerative response to the axonal damage induced by antiretroviral treatment to counteract the antiretroviral neurotoxicity.

4. Microgravity causes substantial down-regulation of nerve tissue proteins, choline acetyltransferase, NF200, and calbindin in mouse motor neurons.

5. Data conclude that NF-H and NF-M C-terminal domains do not normally regulate NF transport rates as previously proposed, but instead increase the proteolytic resistance of NF, thereby stabilizing the stationary neurofilament cytoskeleton along axons.

6. plasma NfH levels closely reflect later stages of disease progression and therapeutic response in the SOD1(G93A) mouse model of amyotrophic lateral sclerosis

7. NFH expression reveals an exquisite level of Cb stripe complexity that respects the transverse zone divisions and delineates an intricately patterned target field for Cb afferents.

8. If expression of neurofilament heavy is developmentally delayed, the neurofilament network is established in a distal to proximal gradient, perhaps to allow distal axonal segments to develop prior to proximal segments.

9. results obtained in mouse model for leprous neuropathy indicate biochemical alterations in neurofilaments, i.e., hyperphosphorylation of NF-H and NF-M

10. Overexpression of peripherin in mice deficient for neurofilament light (NF-L) subunits induced a progressive ALS-like spinal motor neuron degeneration prevented by simultaneous overexpression of NF-H

11. NF-H regulates the association of NFs with kinesin. Phosphorylation of NF-H dissociates NFs from kinesin and provides a mechanism by which NF-H phosphorylation can contribute to the slowing of NF axonal transport.

12. Alpha-internexin coassembles with all 3 neurofilament proteins into a single network of filaments in quadruple-transfected cells. Deleting NF-M alone or together with NF-H in mice reduces alpha-internexin transport & content in axons throughout the CNS.

13. We found an amacrine cell type that was immunolabeled with an antibody against SMI32, a non-phosphorylated epitope on neurofilament proteins of high molecular weight, in the mouse retina.

14. We have validated the serum pNF-H assay as an unbiased measurement of axonal injury in EAE, facilitating rapid screening of potential neuroprotective therapies in this model.

15. RE1 silencing transcription factor (REST) plays a contributory role in the neuron-specific expression of the alpha-internexin, NF-H and NF-M genes

Human Neurofilament, Heavy Polypeptide (NEFH) interaction partners

1. CSF and serum pNfH concentrations are elevated in patients with ALS and correlate with the disease progression rate. Moreover, CSF pNfH correlates with the burden of motor neuron dysfunction.

2. in newborns at risk for hypoxic ischemic encephalopathy but with normal EEGs serum level (phosphorylated) not elevated

3. the regulation of NEFM and NEFH mRNA levels by miRNAs, was investigated.

4. In newborns undergoing cardiac surgery, phosphorylated axonal neurofilament heavy chain was decreased at 0 hours in both the cardiopulmonary bypass and deep hypothermic circulatory arrest groups compared to baseline.

5. Phosphoneurofilament heavy chain level was higher in ALS patients than in controls

6. Data suggest that high CSF NfH levels are an early predictor of later brain and spinal cord atrophy in multiple sclerosis patients.

7. Findings in the dorsolateral prefrontal cortex in schizophrenia provide evidence of altered proteins involved in synaptic function (FABP4), cytoarchitecture organization (NEFH), and circadian molecular clock signaling (CSNK1E), which may be contributing to the cognitive and/or negative symptoms in this disorder. FABP4, CSNK1E and NEFH could become potentially useful biomarkers for schizophrenia.

8. Unique deletions of two nucleotides causing frameshifts near the end of the NEFH coding sequence were identified in two Charcot-Marie-Tooth disease families. Using electroporation of chick embryo spinal cord, confirmed that NEFH mutants form aggregates in vivo and trigger apoptosis of spinal cord neurons.

9. This study confirmed the general applicability of the monocentric obtained cut-off values for neurofilamens in ALS, especially for pNfH

10. data support the use of Cebrospinal fluid phosphorylated NFH as a prognostic biomarker for amyotrophic lateral sclerosis.

11. Study found a significant correlation between values of 8-hydroxy-2'-deoxyguanosine and phosphorylated NF-H only in clinically isolated syndrome group. While the plasma values of 8-hydroxy-2'-deoxyguanosine reflect the degree of acute demyelination in clinically isolated syndrome, phosphorylated NF-H values reflect that in relapsing-remitting multiple sclerosis.

12. Results provide evidence that in particular pNfH can be used as a good diagnostic biomarker of ALS at the diagnostic stage. Moreover, results indicate that NfL may be useful in monitoring disease progression in a subset of patients.

13. Level of neurofilament heavy chain and light chains were significantly elevated in the cerebrospinal fluid of Amyotrophic Lateral Sclerosis (ALS) patients compared to healthy controls/controls without parenchymal central nervous system involvement and ALS mimic disease patients.

14. Study found a significant increase of pNF-H levels in both plasma and CSF in amyotrophic lateral sclerosis patients

15. Studied diagnostic Value of Serum Levels of GFAP, pNF-H, and NSE Compared With Clinical Findings in Severity Assessment of Human Traumatic Spinal Cord Injury.

16. This study demonstrated that Higher NF-L concentrations (beta = -0.26) were associated with functional decline in patients with vascular burden.

17. Phospho-NFH levels were significantly higher in amyotrophic lateral sclerosis patients in comparison with controls, in particular in fast progressors.

18. Data identified NEFH methylation as a candidate epigenetic marker for prognosis of RCC patients as well as prediction of anti-vascular endothelial growth factor-based therapy response.

19. pNFL-H may be useful in determining which individuals require CT imaging to assess the severity of their injury

20. Subconcussive repetitive trauma in amateur boxing causes a mild traumatic brain injury that may be diagnosed by CSF analysis of expressed pNFH, even without unconsciousness or concussion symptoms.