Phospholipase C zeta 1 Proteins (PLCz1)

Pig (Porcine) Phospholipase C zeta 1 (PLCz1) interaction partners

1. These results might shed light on roles of these genes in spermatogenesis as candidate for boar sperm quality and fertility, but still the lack of association across populations should be considered.

2. Results suggest a mechanism by which phospholipase C zeta originates from an oocyte-penetrating assembly--the sperm perinuclear matrix--to induce mammalian oocyte activation at fertilization.

Mouse (Murine) Phospholipase C zeta 1 (PLCz1) interaction partners

1. PLCzeta1 is a sperm-borne oocyte activation factor that ensures oocyte activation steps for monospermic fertilization.

2. analysis of the EF-hand domain in targeting sperm phospholipase Czeta to membrane phosphatidylinositol 4,5-bisphosphate

3. Human PLCzeta exhibits superior fertilization potency over mouse PLCzeta in triggering the Ca(2+) oscillations required for mammalian oocyte activation.

4. these results suggest that both PLCzeta- and sperm-mediated Ca2+ release preferentially use an intracellular membranous source of PI(4,5)P2, in contrast to PLCdelta1, which targets PI(4,5)P2 in the plasma membrane.

5. Data suggest that positively charged residues within the XY linker play an important role in the PLCzeta interaction with PtdIns(4,5)P(2), a crucial step in generating the Ca(2+) activation signal that is essential for fertilization in mammals.

6. Murine PLCZ1 and bovine PLCZ1 possess species-specific differences in activity and suggest potential differences in the mode of action of the protein between the two species.

7. results are consistent with sperm phospholipse C zeta as the molecular trigger for development of a fertilised egg into an embryo

8. purified PLCzeta protein induces Ca(2+) oscillations, indicating that PLCzeta is a proper candidate of the mammalian egg-activating sperm factor

9. Cell cycle-dependent Ca2+ oscillations are regulated by nuclear targeting of PlCzeta.

10. Results suggest a mechanism by which phospholipase C zeta originates from an oocyte-penetrating assembly--the sperm perinuclear matrix--to induce mammalian oocyte activation at fertilization.

11. PLCzeta is the physiological trigger of Ca(2+) oscillations required for activation of development

12. interaction of PI(3)P and PI(5)P could play a role for negative regulation of PLCzeta

13. PLCzeta exhibited alternative cytoplasm/nucleus localization during development

14. These data suggest that the calcium (Ca2+)-dependent features of phospholipase C zeta regulation are essential for it to generate inositol 1,4,5-trisphosphate and Ca2+ oscillations in intact mouse eggs.

15. The role of X/Y linker region and N-terminal EF-hand domain in nuclear translocation and Ca2+ oscillation-inducing activities of phospholipase Czeta.

16. narrow spectrum of PLCZ1 activity indicates that it is modulated by tissue-restricted accessory factors

17. These results support the hypothesis that PLCzeta is the sole mammalian sperm factor and that its linker region may have important regulatory functions during mammalian fertilization.

18. All PLCZ1 proteins including fish could induce Ca(2+) oscillations in mouse eggs, but the activity was variable in the order of human >> mouse > medaka >> rat, estimated from minimal RNA concentration to induce Ca(2+) spikes.

19. Overproduction of DAG in PLCzeta-injected eggs can lead to PKC-mediated Ca(2+) influx and subsequent overloading of Ca(2+) stores.

20. Normal offspring from mouse eggs activated by a phospholipase Czeta protein lacking three EF-hand domains.

Human Phospholipase C zeta 1 (PLCz1) interaction partners

1. PLCzeta and PAWP impairments may be one of the possible etiologies of decreased fertility in Oligoasthenoteratozoospermia

2. Low PLCZ1 expression is associated with globozoospermia with DPY19L2 deletion.

3. we evaluate sperm motility and the proportion of sperm expressing PLC zeta in 71 males (22-54 years; 44 fertile controls and 27 infertile patients), along with total levels and localisation patterns of PLC zeta within the sperm head.

4. findings suggest that human PLCZ1 RNA is a better therapeutic agent to rescue human oocytes from failed activation, leading to normal and efficient development.

5. The value of PAWP and PLCzeta as indicators of sperm quality was studied.

6. The role of the PLCZ1 protein in male infertility, and its expression regulation and therapeutic potential have been discussed. (Review)

7. latest developments that have begun to unravel the vital role of PLCzeta at mammalian fertilisation and decipher its unique mechanism of action within the fertilising egg.

8. The findings highlight the importance of PLCzeta at fertilization and the vital role of the C2 domain in PLCzeta function, possibly due to its novel binding characteristics.

9. The findings of the present study illustrate that one of the etiologies of reduced fertility associated with varicocele is the low expression of PLCzeta

10. In male infertility, the absence of PLCZ1 alone is sufficient to prevent oocyte activation irrespective of the presence of PAWP.

11. Human PLCzeta elicited the characteristic Ca(2+) oscillations present at mammalian fertilization, which produced oocyte activation and embryo development.

12. Study further supports the fundamental role of PLCzeta in the oocyte activation process.

13. Phospholipase C-zeta deficiency is associated with repetitive oocyte fertilization failure during ovarian stimulation for in vitro fertilization.

14. I discuss the recent advances in our knowledge of the intriguing biochemical and physiological properties of sperm PLCz and postulate potential roles for PLCz in terms of clinical diagnosis and therapy for certain forms of male infertility

15. Data suggest that PAWP (WW domain-binding protein 2-like) from sperm (rather than PLCZ [phospholipase C zeta] from sperm) acts as the 'sperm factor' within the egg at fertilization and is involved in 'egg activation' and embryogenesis. [REVIEW]

16. in patients with normal SA parameters but with repeated low fertilization or outright failed fertilization results after ICSI, abnormal PLCZ1 function should be considered as the underlying mechanism responsible for the failure of fertilization

17. Human PLCzeta exhibits superior fertilization potency over mouse PLCzeta in triggering the Ca(2+) oscillations required for mammalian oocyte activation.

18. Male infertility is associated with the aberrant expression, localisation, structure and function of PLCzeta in human sperm. [review]

19. PLCzeta may be a significant factor in human fertility with potential therapeutic capacity. [Review]

20. investigation of expression level and localization pattern of PLCZ1 in sperm from three infertile patients with globozoospermia (i.e., patients who exhibited abnormal sperm with round heads) [CASE REPORT]

Cow (Bovine) Phospholipase C zeta 1 (PLCz1) interaction partners

1. Data show that the level of actin/PLC-zeta interaction was twice as high in G-actin that reflected an increase in affinity.

2. A novel splice variant of Phospholipase C zeta 1 in the testis tissues of adult and fetal Chinese Holstein bulls was characterized.

3. Murine PLCZ1 and bovine PLCZ1 possess species-specific differences in activity and suggest potential differences in the mode of action of the protein between the two species