Piezo-Type Mechanosensitive Ion Channel Component 1 Proteins (PIEZO1)

Human Piezo-Type Mechanosensitive Ion Channel Component 1 (PIEZO1) interaction partners

1. The membrane footprint amplifies the sensitivity of piezo-type mechanosensitive ion channel component 1 (Piezo1) to changes in membrane tension, rendering it exquisitely responsive.

2. A family with clinical and laboratory characteristics of hereditary xerocytosis (HX) had an apparently conservative novel variant, L2023V, in PIEZO1. Functional analyses demonstrated a gain-of-function phenotype for the mutant PIEZO1 channel, confirming the pathogenicity of the variant and its causative role for HX.

3. PIEZO1 exhibited an upregulation in most of the Gastric Cancer cell lines and primary samples compared with non-tumorous gastric epithelial tissues. Increase of PIEZO1 was associated with poor disease specific survival. PIEZO1 knockdown led to inhibitory effect by suppressing cell proliferation and invasion and inhibiting xenograft formation.

4. Whole-cell current from coexpressed PIEZO1/PIEZO2 also fit as a linear combination of homomer currents. High-resolution optical images of fluorescently-tagged channels support this interpretation because coexpressed subunits segregate into discrete domains.

5. Study using cultured human and mouse cells found that margaric acid, a saturated fatty acid present in dairy products and fish, inhibits Piezo1 activation and polyunsaturated fatty acids (PUFAs), present in fish oils, modulate channel inactivation.

6. rare genetic variation accounts for a genetic phenotype-modifier role in dehydrated hereditary stomatocytosis

7. we discovered a hitherto unknown regulatory mechanism for human T cell activation and provide the first evidence, to our knowledge, for the involvement of Piezo1 mechanosensors in immune regulation.

8. Results find that Ucn1 exerts its chondroprotective effect by maintaining Piezo1 in a closed conformation.

9. Cell surface flip-flop of phosphatidylserine acts as a molecular switch for PIEZO1 activation that governs proper morphogenesis during myotube formation.

10. Both vertebrate and invertebrate mechanosensitive PIEZO ion channels are polymodal, being voltage modulated, and can even be directly voltage gated following a preceding mechanical stimulus.

11. In the absence of extracellular matrix (ECM) proteins Piezo1 receptors are relatively insensitive to mechanical forces pushing the cellular membrane, whereas they can hardly be activated by mechanically pulling the membrane. Yet, if conjugated with Matrigel, a mix of ECM proteins, the receptors become sensitized.

12. present study was designed to evaluate in hereditary xerocytosis the functional link between mutated Piezo1 and KCNN4

13. the knockdown of PIEZO1 significantly reduced the cell-viability of SW982 cells.

14. analysis of Piezo1 domains by using localized force on magnetic nanoparticles

15. Piezo1-shRNA could inhibit the invasion of the osteosarcoma cells.

16. MiR-103a might be a potential biomarker of myocardium infarction and could be used as an index for the diagnosis of AMI. It may be involved in the development of HBP and onset of AMI through regulating the Piezo1 expression.

17. The structural dynamics of the PIEZO1 channel activation and inactivation by coarse-grained modeling has been reported.

18. Additional alterations in mutant PIEZO1 channel kinetics, differences in response to osmotic stress, and altered membrane protein trafficking, predicting variant alleles that worsen or ameliorate erythrocyte hydration.

19. differential regulation of PKA and cell stiffness in unconfined versus confined cells is abrogated by dual, but not individual, inhibition of Piezo1 and myosin II.

20. data demonstrate that PIEZO1 is required for the regulation of NO formation, vascular tone, and blood pressure.

Mouse (Murine) Piezo-Type Mechanosensitive Ion Channel Component 1 (PIEZO1) interaction partners

1. PIEZO1 knockdown led to inhibitory effect by suppressing cell proliferation and invasion and inhibiting xenograft formation.

2. Study using cultured human and mouse cells found that margaric acid, a saturated fatty acid present in dairy products and fish, inhibits Piezo1 activation and polyunsaturated fatty acids (PUFAs), present in fish oils, modulate channel inactivation.

3. mechanosensitive channels such as Piezo1, located in close proximity to hormone-release sites, enable G cells to respond directly to antrum distensions with gastrin secretion.

4. Piezo1 knocked down by siRNA can inhibit the migration ability of MC3T3-E1 osteoblast cells.

5. Mechanically activated ion channel PIEZO1 is a key regulator of lymphatic valve formation.

6. Piezo1 knockout embryonic stem cells showed a reduced rate of proliferation but no significant differences in other markers of pluripotency and differentiation.

7. Cell surface flip-flop of phosphatidylserine acts as a molecular switch for PIEZO1 activation that governs proper morphogenesis during myotube formation.

8. Piezo1 is a mechanically activated ion channel that mediates pressure induced pancreatitis.

9. Study results show that the Piezo1 pore remains fully open if only one subunit remains activated. This study sheds light on the gating and pharmacological mechanisms of a member of the Piezo channel family.

10. These findings suggest that PIEZO1 and PIEZO2 are the long-sought baroreceptor mechanosensors critical for acute blood pressure control.

11. In the absence of extracellular matrix (ECM) proteins Piezo1 receptors are relatively insensitive to mechanical forces pushing the cellular membrane, whereas they can hardly be activated by mechanically pulling the membrane. Yet, if conjugated with Matrigel, a mix of ECM proteins, the receptors become sensitized.

12. three-bladed, propeller-like electron cryo-microscopy structure of mouse Piezo1 and functional revelation of its mechanotransduction components

13. high-resolution cryo-electron microscopy structure of the mouse Piezo1 trimer

14. Piezo ion channels act as frequency filters of both the onset and continuation of repetitive mechanical stimuli.

15. Both TRPV4 and PIEZO1 channels contribute to currents activated by stimuli applied at cell-substrate contacts but only PIEZO1 mediates stretch-activated currents. These data demonstrate that there are separate, but overlapping, mechanoelectrical transduction pathways in chondrocytes.

16. Piezo1 is a molecular sensor of physical exercise in the endothelium that triggers endothelial communication to mesenteric vessel muscle cells, leading to vasoconstriction.

17. mechanical perturbations of the lipid bilayer alone are sufficient to activate Piezo channels, illustrating their innate ability as molecular force transducers.

18. data demonstrate that PIEZO1 is required for the regulation of NO formation, vascular tone, and blood pressure.

19. clock genes exist in the mouse bladder mucosa and regulate exact circadian gene expression in mice

20. Our results suggest that Piezo1 is unlikely to be a component of the mechanoelectrical transducer channel complex in mammalian cochlear outer hair cells