Septin 12 Proteins (Sep12)

Human Septin 12 (Sep12) interaction partners

1. the current work identified the promoter of the human SEPT12 gene and provided key evidence about its transcriptional regulation via E2 and 5alpha-DHT.

2. In mature human spermatozoa, SEPT12 and NDC1 are majorly colocalized in the centrosome regions; however, NDC1 is only slightly co-expressed with SEPT12 at the annulus of the sperm tail.

3. results demonstrate the molecular architecture of SEPT12 filaments at the sperm annulus, their mechanical support of sperm motility, and their correlation with male infertility.

4. we discovered that the SEPTIN12-microtubule complexes are critical for sperm formation during spermiogenesis

5. SEPTIN12 genetic variants confer susceptibility to teratozoospermia

6. Eight coding single-nucleotide polymorphisms in SEPTIN12 were detected in the patients with Sertoli cell-only syndrome. Analysis of the results suggest that SEPTIN12 might play a critical role in human spermatogenesis.

7. SEPT12 mutations cause male infertility with defective sperm annulus and disrupt sperm structural integrity

8. The c.204G>C (Gln38His) variant in the SEPTIN12 gene was associated with increased susceptibility to azoospermia caused by meiotic arrest

9. While SEPT12 formed filamentous structures at interphase, it was localized to the central spindle and to midbody during anaphase and cytokinesis, respectively.

10. Decreases in SEPTIN12 expression is associated with male infertility.

Mouse (Murine) Septin 12 (Sep12) interaction partners

1. We concluded that CDC42 negatively regulates SEPT12 polymerization and is involved in terminal structure formation of sperm heads.

2. Data suggest that SEPT12 phosphorylation inhibits septin ring assembly during annulus biogenesis.

3. In mice sperm with mutated SEPT12, NDC1 dispersed around the manchette region of the sperm head and annulus, compared with concentrating at the sperm neck of wild-type sperm.

4. results demonstrate the molecular architecture of SEPT12 filaments at the sperm annulus, their mechanical support of sperm motility, and their correlation with male infertility.

5. Oocytes fertilized with spermatozoa obtained from Septin 12+/- chimeric mice failed to develop beyond the morula stage after IVF and intracytoplasmic sperm injection because of significant DNA defects in the spermatozoa