Sodium Channel, Nonvoltage-Gated 1, gamma Proteins (SCNN1G)

Xenopus laevis Sodium Channel, Nonvoltage-Gated 1, gamma (SCNN1G) interaction partners

1. Plasmin in nephrotic urine activates the epithelial sodium channel

Human Sodium Channel, Nonvoltage-Gated 1, gamma (SCNN1G) interaction partners

1. Albuminuria in kidney transplant recipients is associated with hypertension, ability of urine to proteolytically activate ENaC current, and increased abundance of gammaENaC.

2. Proteolytic cleavage activates epithelial sodium channel (SCNN) by removal of specific segments in finger domains of alpha and gamma subunits. Data suggest that the lower "palm" domain of SCNN1A is essential for allosteric activation of the SCNN heterotetramer [SCNN1A(1)-SCNN1B-SCNN1A(2)-SCNN1G].

3. In the present study, the SCNN1G gene was associated with 24-h urinary sodium excretion but was not related to blood pressure. The SCNN1A and SCNN1B genes were not associated with blood pressure either.

4. The derlin-1 pathway therefore may represent a significant early checkpoint in the recognition and degradation of ENaC in mammalian cells.

5. hENaC incorporating the Liddle-mutated beta-subunit lacks one or more PKC phosphorylation sites, thereby significantly reducing the inhibitory effect of PKC on Na(+) channel activity, whereas hENaC incorporating Liddle-mutated gamma-subunits remains as susceptible to PKC as wild-type hENaC.

6. deltabetagamma-ENaC is inhibited by CFTR but activated by cyclic AMP.

7. One nonsynonymous amino acid variants in SCNN1G in nonwhite Cystic fibrosis patients with non-diagnostic CFTR genotypes was identified.

8. Both vascular smooth muscle cell and bronchial gamma-ENaC downregulation may explain pulmonary fluid disturbances and participate in pulmonary edema pathogenesis.

9. The transcriptional expression of alpha, beta, and gamma subunits of ENaC was elevated in nasal polyp compared to nasal mucosa

10. Suggest that SCNN1G gene has important roles in blood pressure regulation in the Han Chinese population.

11. SCNN1A and SCNN1G SNPs may contribute to blood pressure changes over time in the Han Chinese population.

12. Data indicate that urokinase-type plasminogen activator (uPA) activates epithelial sodium channel, gamma subunit (ENaC gamma) through catalytic activity-dependent proteolytic modification.

13. High serum leptin may reduce endometrial receptivity by activating the STAT3 signal pathway and down-regulating gamma-ENaC expression in the endometrium.

14. human kidney gammaENaC is subject to proteolytic cleavage, yielding fragments compatible with furin cleavage, and proteinuria is associated with cleavage at the putative prostasin/kallikrein site and removal of the inhibitory tract within gammaENaC

15. gamma subunit palmitoylation has a dominant role over beta subunit palmitoylation in modulating ENaC gating

16. WNK4 inhibits ENaC channel activity independently of Nedd4-2-mediated ENaC ubiquitination.

17. found a diminished expression of the three subunits of the ENaC in the membranes of preeclamptic placentas in comparison with the normal ones

18. These results suggest that the mutant gammaL511Q enhances ENaC activity by increasing channel open probability and dampens channel regulation by extracellular Na(+) and proteases.

19. analysis of eight extracellular domain residues in human beta- and gammaENaC that are required for regulation by acidic pH

20. Mutating two valine residues (V182 and V193) in the critical region of gammaENaC prevented proteolytic activation of ENaC by Cat-S.

Mouse (Murine) Sodium Channel, Nonvoltage-Gated 1, gamma (SCNN1G) interaction partners

1. the results of the present study demonstrated that insulin protected the lung epithelium and attenuated pulmonary edema through the upregulation of ENaC via the PI3K/SGK1 pathway in LPSinduced lung injury.

2. Casein kinase 2 phosphorylation is an important regulator of ENaC activity and sodium handling by the aldosterone sensitive distal nephron.

3. N-linked glycans on each subunit are required for proper folding, maturation, surface expression, and function of the epithelial sodium channel.

4. The derlin-1 pathway therefore may represent a significant early checkpoint in the recognition and degradation of ENaC in mammalian cells.

5. ENaC and ROMK channel activity in kidney tubules are inhibited in TgWnk4(pseudoaldosteronism type II) mice. Wnk4(PHAII)-induced inhibition of ENaC and ROMK may contribute to the suppression of K(+) secretion in the tubules.

6. Mouse cells conditionally overexpressing gamma ENAC had enhanced apical Na(+) entry increased the basolateral cell surface expression of Na,K-ATPase by inhibiting p38 kinase-mediated endocytosis of Na,K-ATPase.

7. Data indicate that insulin receptor (InsR) is important for epithelial sodium channel (ENaC) activity.

8. These data suggest that adenylyl cyclase VI mediates vasopressin-stimulated ENaC activity in the kidney.

9. Cys substitutions at sites in the wrist domains of the beta and gamma subunits alter the Na+ self-inhibition response.

10. ENaC is not a passive passenger in regulated epithelial vesicle trafficking, but plays a role in establishing and maintaining the pool of vesicles that respond to cAMP stimulation.

11. The reactive intermediates formed during and after exposure to chlorine activated ERK1/2 in alveolar epithelial cells, leading to decreased ENaC concentrations and activity.

12. TMPRSS4 primarily activates ENaC by cleaving basic residues within the tract gammaK173-K186 distal to the furin cleavage site, thereby releasing a previously defined key inhibitory tract encompassing gammaR158-F168 from the gamma-subunit.

13. estrogen regulates expression and function of epithelial sodium channel gamma in osteoblasts

14. Il-6 activates ENaC-gamma channels in cortical collecting duct cells.

15. Dot1a and AF9 downregulate Na(+) transport, most likely by regulating ENaC mRNA and subsequent protein expression and ENaC activity.

16. pore regions from the three ENaC subunits have an asymmetric organization

17. Alpha-, beta-, or gamma-ENaC coimmunoprecipitated with myc-tagged alpha-alpha-M1H1 and its downstream hydrophilic extracellular loop. Alpha-subunit participates in subunit-subunit interactions required for assembly of heterooligomeric ENaC complex.

18. ATP regulation of ENaC may be involved in the fine-tuning of ENaC activity under physiologic and pathophysiologic conditions

19. residues within Gln573-Arg583 of gammaENaC are necessary for normal channel gating

20. mENaC is a substrate for furin and mENaC cleavage correlates with channel activity. Cleavage is blocked by mutation of the furin consensus sites in alpha and gamma subunits, indicating cleavage of both alpha and gamma within the extracellular loop (C)