Monensin Solution (1,000X)
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- Application
- Flow Cytometry (FACS)
- Characteristics
- Monensin is a protein transport inhibitor commonly used to enhance intracellular cytokine staining signals by blocking transport processes during cell activation. Especially useful for the intracellular staining of cytokines, monensin leads to the accumulation of most cytokines at the Golgi Complex/Endoplasmic Reticulum (see Jung, et al., 1993). Optimal conditions for use are cell type and time-dependent. Typically, protein transport inhibitors are included during in vitro cell activation cultures for 4-24 hours prior to harvest (see references below for additional information). Monensin Solution is supplied as a 1,000X solution, which should be diluted to 1X in cell culture medium.
- Components
- Monensin Solution (1,000X)
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 2.0 mM
- Buffer
- Monensin Solution is supplied as a 1000X working solution in 70 % Ethanol. Dilute to 1X in cell culture medium.
- Storage
- 4 °C
- Storage Comment
- Store the solution between 2°C and 8°C.
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