Mab anti-Human HSP27 antibody (clone G3.1) is shown to detect human HSP27 by western blot. Detection occurs after 10 µg of a HeLa whole cell lysate is loaded per lane. The blot was incubated with a 1:1,000 dilution of Mab anti-Human HSP27 at room temperature for 30 min followed by detection using800 labeled Goat-a-Mouse IgG [H&L] diluted 1:5,000. A single band corresponding to human HSP27 is detected at ~27 kDa when compared with known molecular weight standards (not shown). The antibody may be used to detect endogenous human HSP27.800 fluorescence image was captured using the Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Mab anti-Human HSP27 antibody (clone G3.1) is shown to detect human HSP27 by western blot. Detection occurs after 10 µg of a HeLa whole cell lysate is loaded per lane. The blot was incubated with a 1:1,000 dilution of Mab anti-Human HSP27 at room temperature for 30 min followed by detection using800 labeled Goat-a-Mouse IgG [H&L] diluted 1:5,000. A single band corresponding to human HSP27 is detected at ~27 kDa when compared with known molecular weight standards (not shown). The antibody may be used to detect endogenous human HSP27.800 fluorescence image was captured using the Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Mab anti-Human HSP27 antibody (clone G3.1) is shown to detect human HSP27 by western blot. Detection occurs after 10 µg of a HeLa whole cell lysate is loaded per lane. The blot was incubated with a 1:1,000 dilution of Mab anti-Human HSP27 at room temperature for 30 min followed by detection using800 labeled Goat-a-Mouse IgG [H&L] diluted 1:5,000. A single band corresponding to human HSP27 is detected at ~27 kDa when compared with known molecular weight standards (not shown). The antibody may be used to detect endogenous human HSP27.800 fluorescence image was captured using the Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Mab anti-Human HSP27 antibody (clone G3.1) is shown to detect human HSP27 by western blot. Detection occurs after 10 µg of a HeLa whole cell lysate is loaded per lane. The blot was incubated with a 1:1,000 dilution of Mab anti-Human HSP27 at room temperature for 30 min followed by detection using800 labeled Goat-a-Mouse IgG [H&L] diluted 1:5,000. A single band corresponding to human HSP27 is detected at ~27 kDa when compared with known molecular weight standards (not shown). The antibody may be used to detect endogenous human HSP27.800 fluorescence image was captured using the Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
