HSPB1 antibody (Heat Shock 27kDa Protein 1)

Details for Product anti-HSPB1 Antibody No. ABIN100223
Antigen
Reactivity
Human
786
304
285
79
73
60
59
54
47
34
29
24
9
8
7
6
4
3
2
1
1
1
Host
Mouse
580
228
1
Clonality (Clone)
Monoclonal ()
Conjugate
This HSPB1 antibody is un-conjugated
36
27
16
15
14
14
14
14
14
14
11
11
6
6
6
6
6
5
4
4
3
3
3
3
3
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3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
638
299
236
203
156
139
128
115
79
38
32
21
18
14
13
8
6
4
2
2
1
1
Options
Immunogen This HSP27 monoclonal antibody was produced by repeated immunizations with a prokaryotic recombinant protein corresponding to the full length human hsp27 protein.
Immunogentype:Recombinant
Clone G3-1
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Sterility Sterile filtered
Antigen
Alternative Name HSP27 (HSPB1 Antibody Abstract)
Background Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in different cell types and tissues (this protein has also been referred to as the Estrogen-Regulated 24 kDa protein, hsp25 and hsp28).   Like other small heat shock proteins, HSP27 is regulated at both the transcriptional and post-translational level.  In response to stress, the expression level of HSP27 increases several-fold to confer cellular resistance to the adverse environmental change.  Human HSP27 shares greater than 50% homology with the low molecular weight Drosophila HSP and mammalian crystalline lens protein.  Because of the estrogen responsive nature of HSP27, the protein has been extensively
Synonyms: Heat shock protein beta-1 antibody, HspB1 antibody, Heat shock 27 kDa protein antibody, Stress-responsive protein 27 antibody, SRP27 antibody, Estrogen-regulated 24 kDa protein antibody, 28 kDa heat shock protein antibody
Gene ID 39078, 17647521
UniProt P04792
Pathways MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
Application Notes This protein A purified monoclonal antibody against human Hsp27 has been tested for use in immunoblotting, immunoprecipitation, ELISA, immunohistochemistry and immunocytochemistry.  The antibody recognizes a 27 kDa band corresponding to hsp27 in cell lysates from breast carcinoma.    Both frozen sections and paraffin embedded material can be used for immunocytochemistry and immunohistochemistry.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Image no. 1 for anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (ABIN100223) Mab anti-Human HSP27 antibody (clone G3.1) is shown to detect human HSP27 by western ...
Product cited in: McGuire, Fuqua, Naylor, Helin-Davis, McGuire: "Chromosomal assignments of human 27-kDa heat shock protein gene family." in: Somatic cell and molecular genetics, Vol. 15, Issue 2, pp. 167-71, 1989 (PubMed).

Moretti-Rojas, Fuqua, Montgomery, McGuire: "A cDNA for the estradiol-regulated 24K protein: control of mRNA levels in MCF-7 cells." in: Breast cancer research and treatment, Vol. 11, Issue 2, pp. 155-63, 1988 (PubMed).

Edwards, Adams, McGuire: "Estradiol stimulates synthesis of a major intracellular protein in a human breast cancer cell line (MCF-7)." in: Breast cancer research and treatment, Vol. 1, Issue 3, pp. 209-23, 1983 (PubMed).