Goat anti-Human IgA (Heavy Chain) Antibody (TRITC) - Preadsorbed

Details for Product No. ABIN100721
Antigen
Epitope
Heavy Chain
69
49
23
11
10
6
6
1
1
Reactivity
Human
126
60
38
28
13
11
10
10
10
10
6
5
4
4
3
3
3
3
2
2
1
1
1
1
Host
Goat
142
88
57
13
7
7
2
Clonality
Polyclonal
Conjugate
TRITC
37
34
32
21
19
15
6
5
4
4
4
4
4
4
4
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
FLISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
Options
Immunogen

Immunogen: Human IgA alpha heavy chain

Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Purification Preadsorption: Solid phase absorption
Antigen
Background

Synonyms: goat anti-Human IgA (alpha chain) rhodamine conjugated Antibody, goat anti-Human IgA alpha Antibody TRITC conjugation

Background: Anti-Human IgA Rhodamine Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Application Notes

Application Note: Anti-Human IgA Rhodamine Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:500 - 1:2,500

IF Microscopy Dilution: 1:1,000 - 1:5,000

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 2.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Product is photosensitive and should be protected from light.
Storage RT,4 °C,-20 °C
Expiry Date 12 months