Rabbit anti-Human IgA (Heavy Chain) Antibody (Biotin) - Preadsorbed

Catalog No. ABIN100724

Product Details

Target: IgA

Binding Specificity: Heavy Chain

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Human IgA alpha heavy chain

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:250 - 1:1,000

Application Note: This product has been assayed against 1.0 μg of Human IgA in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

ELISA Dilution: 1:5,000 - 1:20,000

Western Blot Dilution: 1:500 - 1:2,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: RT,4 °C,-20 °C

Expiry Date: 12 months

Target Details

Target: IgA

Abstract: IgA Products

Target Type: Antibody

Background:

Synonyms: Rabbit Anti Human IgA (alpha chain) Antibody Biotin Conjugated, Rabbit Anti-Human IgA (alpha chain) Antibody Biotin Conjugated, Rabbit Anti Human IgA Antibody Biotin Conjugated

Background: Anti-Human IgA Biotin Antibody generated in rabbit detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.