Immunohistochemistry Dilution: 1:250 - 1:1,000
Application Note: This product has been assayed against 1.0 μg of Human IgA in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5,000 - 1:20,000
Western Blot Dilution: 1:500 - 1:2,000